Biological control of Pectobacterium carotovorum subsp. carotovorum, the causal agent of bacterial soft rot in vegetables, in vitro and in vivo tests

Abstract

Background Several chemical bactericides were applied for controlling soft rot bacteria, Pectobacterium carotovorum subsp. carotovorum, which causes the destructive soft rot disease to many economically important vegetables, but because of their toxic hazards on human and environment became limit. The biocontrol was applied to control many plant pathogens. Therefore, this work is aimed to study the antagonistic activity of bacterial agents, i.e. Bacillus subtilis, Bacillus pumilus, Bacillus megaterium and Pseudomonas fluorescens, and fugal agents, i.e. Trichoderma harzianum, Trichoderma viride and Trichoderma virens, to control bacterial soft rot disease under in vitro and in vivo tests. Results The tested treatments could protect the potato tubers against the development of soft rot. T. viride and T. virens were highly effective in reducing soft rot symptoms on inoculated potato tuber slices, when applied at the same time or 2 h before pathogen inoculation, while B. megaterium and T. harzianum were highly effective when applied at the same time or 2 h after pathogen inoculation. In whole potato tubers technique, B.pumilus highly protected the stored potato tuber under artificially infection conditions, than P. fluorescens, T. harzianum, B. subtilis, T. viride, T. virens and B. megaterium, respectively. Conclusion Application of fungal agents or specify the bacterial species can play an important role in controlling bacterial soft rot disease in vegetables and increase the stored periods of potato tubers under storage conditions without any toxic effects.

An insight into the role of carbon dots in the agriculture system: a review

“…carotovora ) is a phytopathogen responsible for the development of soft rot and stem rot diseases in several crops such as Chinese cabbage, potato, and tomato. 231 Agrobacterium tumefaciens , a soil-born bacterium, is responsible for crown gall disease in woody plants such as apples, cherry, walnuts, grapevines, rose, etc. , and occasionally in crops such as cotton, sugar beet, tomatoes, beans, and alfalfa.…”

Synthesis and diverse biological activity profile of triethylammonium isatin-3-hydrazones

“…Bacterial pathogens isolated from the plants microenvironment and harvested vegetables frequently include Micrococcus luteus, Pseudomonas fluorescens, Pectobacterium carotovorum (Erwinia carotovora), Xanthomonas campestris, and Pectobacterium atrosepticum (Erwinia carotovora subsp. atrosepticum) [38][39][40]. Fungal pathogens cause 70-80 % of all plant diseases [41], possessing a potentiality of causing large-scale disease outbreaks in a very limited period of time.…”

Soft rot of postharvest pepper: bacterial pathogen, pathogenicity and its biological control using Lactobacillus farciminis LJLAB1

“…Capsaicin from pepper has been used in the food industry, agriculture, medical clinics, cosmetics and so forth 2 . However, pepper is susceptible to microbial infection after harvest during storage and transportation especially when fruit is injured in the field by insect feeding, which has caused serious economic losses 3 . Pathogens of postharvest pepper include bacteria and fungi, but research mainly focuses on controlling fungal diseases at present 4,5 .…”

“…2 However, pepper is susceptible to microbial infection after harvest during storage and transportation especially when fruit is injured in the field by insect feeding, which has caused serious economic losses. 3 Pathogens of postharvest pepper include bacteria and fungi, but research mainly focuses on controlling fungal diseases at present. 4,5 The isolation, identification and control of bacterial pathogens of postharvest pepper have not been comprehensively reported, especially bacterial soft rot of pepper.…”