Biological control of powdery mildew on <i>Cornus florida</i> using endophytic <i>Bacillus thuringiensis</i>

Rotich, Emily; Mmbaga, Margaret T.; Joshua, Jacqueline

doi:10.1080/07060661.2019.1641555

Cited by 16 publications

(10 citation statements)

References 26 publications

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“…The bacterial endophytes used in our study were selected for biological control of fungal pathogens on diverse plants (Irabor and Mmbaga, 2017;Joshua and Mmbaga, 2020;Mmbaga et al, 2008Mmbaga et al, , 2016Mmbaga et al, , 2018Rotich et al, 2019). In addition to disease control, the endophytes exhibited plant growth promotion.…”

Section: Discussionmentioning

confidence: 99%

“…Several bacterial endophytes have been isolated and identified in our earlier studies (Joshua and Mmbaga, 2020;Mmbaga et al, 2008). In our previous studies, endophytes isolated from flowering dogwood, papaya, and common beans were shown to exhibit biocontrol activities against fungal diseases such as powdery mildew of flowering dogwood (Mmbaga et al, 2008(Mmbaga et al, , 2016Rotich, 2015;Rotich et al, 2019), phytophthora blight of pepper (Bhusal and Mmbaga, 2020;Irabor and Mmbaga, 2017;Mmbaga et al, 2018a), and macrophomina root rot (Joshua, 2017;Joshua and Mmbaga, 2020;Mmbaga et al, 2018b). In addition, the selected bacterial isolates applied to plants using root drenching, foliage sprays, and seed treatment displayed plant growth-promoting abilities.…”

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confidence: 99%

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Effect of Volatile Compounds Produced by Selected Bacterial Endophytes in Promoting Plant Growth

Maheshwari¹,

Mmbaga²,

Bhusal³

et al. 2021

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Bacterial endophytes selected for their capability to suppress diverse fungal pathogens in vitro and in greenhouse studies have been shown to promote plant growth. The effect of volatile compounds emitted by selected bacteria on plant growth in Arabidopsis thaliana, tomato (Solanum lycopersicum), sweet pepper (Capsicum annuum), and cucumbers (Cucumis sativus) was evaluated on container-grown plants nested above bacterial cultures, with roots exposed to the volatiles without direct contact between bacterial cells and the plant roots. Significant increases in plant growth were observed in plant height, root length, leaf size, fresh weight, and chlorophyll content in all plants tested. Although diverse chemical compounds may be involved in promoting plant growth, including volatile and nonvolatile compounds, observations in this study have implications for the potential role of the selected bacteria in plant production as biofertilizers and biopesticides.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Effect of Volatile Compounds Produced by Selected Bacterial Endophytes in Promoting Plant Growth

Maheshwari¹,

Mmbaga²,

Bhusal³

et al. 2021

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“…Screening of endophytes as BCAs of fungal pathogens. Eight bacterial endophytes including five presented in Table 2 and three (B17A, B17B, and IMC8) previously isolated from C. florida stem pieces and found to suppress Erysiphe pulchra (Mmbaga et al, 2018b;Rotich et al, 2019), were evaluated for antimicrobial activity against 11 soilborne fungal pathogens presented in Table 1 and Glomerella acutata previously isolated as a soilborne pathogen. The antimicrobial activity was assessed using a dual culture technique in which bacterial endophytes and fungal pathogens were placed at opposite sides of the petri plates containing both potato dextrose agar (PDA) and NA at 1:1 v/v ratio to support growth of both the fungal pathogens and the bacterial endophytes.…”

Section: Methodsmentioning

confidence: 99%

Potential Biological Control Agents for Soilborne Fungal Pathogens in Tennessee Snap Bean Farms

Joshua¹,

Mmbaga²

2020

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Fungi isolated from snap bean roots and rhizosphere soil where fungicides are not used included Fusarium oxysporum, Fusarium equiseti, Fusarium subglutinans, Fusarium camptoceras, Fusarium chlamydosporum, Fusarium verticillioides, Fusarium proliferatum, Fusarium acuminatum, Fusarium solani, Peyronellaea pinodella, Macrophomina phaseolina, and Glomerella guttata. Only P. pinodella, M. phaseolina, and F. oxysporum were isolated on symptomatic plants. These soilborne fungi are common pathogens of diverse host plants. Pathogenicity tests under controlled environment demonstrated that these fungi were pathogenic on snap beans. Subsequently, bacterial endophytes isolated from snap bean roots, papaya roots and stems, and dogwood stems were evaluated as potential biological control agents against these diverse fungi. All bacteria isolated, including Bacillus vallismortis (PS), Bacillus amyloliquefaciens (Psl), Bacillus subtilis (Prt), Bacillus thuringiensis (Y and IMC8), Enterobacter sp. (E), Stenotrophomonas sp. (B17A), and Serratia sp. (B17B) suppressed growth of the fungal pathogens in vitro and formed clear inhibition zones in petri dish dual cultures. Growth media taken from the inhibition zones suppressed growth of the fungal pathogens in the absence of the bacterial cells, suggesting that the bacteria released unidentified antagonistic biochemical substances into the media. This study constitutes an initial screening of endophytes as biological control agents against diverse fungal pathogens and forms a basis for the discovery of novel strains that can be further developed and integrated into disease management systems for diverse fungal pathogens. Isolates B. vallismortis (PS), B. amyloliquefaciens (Psl), B. subtilis (Prt), and B. thuringiensis (Y IMC8) exhibited the best performance as potential biological control agents paving the way for larger-scale in vivo studies and characterization of their interactions with fungal pathogens.

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“…Several endophyte BCAs have been isolated and identified in our earlier studies (Joshua & Mmbga, 2019 ; Mmbaga et al, 2008 ). In these studies, BCAs isolated from flowering dogwood, papaya, pepper, and snap beans were shown to exhibit biocontrol activities against fungal diseases (Irabor & Mmbaga, 2017 ; Joshua, 2017 ; Mmbaga et al, 2008 , 2016 ; Mmbaga, Gurung, & Maheshwari, 2018 ; Mmbaga, Mackasmiel, & Mrema, 2018 ; Rotich, 2015 ; Rotich et al, 2020 ) and actively produce secondary metabolites for antagonism of plant pathogens (Compant et al, 2005 ; Raaijmakers et al, 2002 ). They were also shown to produce various antimicrobial secondary metabolites, which can inhibit the growth of fungal pathogens.…”

Section: Introductionmentioning

confidence: 99%

“…In these studies, BCAs isolated from flowering dogwood, papaya, pepper, and snap beans were shown to exhibit biocontrol activities against fungal diseases (Irabor & Mmbaga, 2017;Joshua, 2017;Mmbaga et al, 2008Mmbaga et al, , 2016Mmbaga, Gurung, & Maheshwari, 2018;Mmbaga, Mackasmiel, & Mrema, 2018;Rotich, 2015;Rotich et al, 2020) and actively produce secondary metabolites for antagonism of plant pathogens (Compant et al, 2005;Raaijmakers et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Efficient fluorescence‐based localization technique for real‐time tracking endophytes route in host‐plants colonization

Ondzighi‐Assoume

Bhusal

et al. 2022

Plant Direct

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Bacterial isolates that enhance plant growth and suppress plant pathogens growth are essential tools for reducing pesticide applications in plant production systems. The objectives of this study were to develop a reliable fluorescence‐based technique for labeling bacterial isolates selected as biological control agents (BCAs) to allow their direct tracking in the host‐plant interactions, understand the BCA localization within their host plants, and the route of plant colonization. Objectives were achieved by developing competent BCAs transformed with two plasmids, pBSU101 and pANIC‐10A, containing reporter genes eGFP and pporRFP , respectively. Our results revealed that the plasmid‐mediated transformation efficiencies of antibiotic‐resistant competent BCAs identified as PSL, IMC8, and PS were up 84%. Fluorescent BCA‐tagged reporter genes were associated with roots and hypocotyls but not with leaves or stems and were confirmed by fluoresence microscopy and PCR analyses in colonized Arabidopsis and sorghum. This fluorescence‐based technique's high resolution and reproducibility make it a platform‐independent system that allows tracking of BCAs spatially within plant tissues, enabling assessment of the movement and niches of BCAs within colonized plants. Steps for producing and transforming competent fluorescent BCAs, as well as the inoculation of plants with transformed BCAs, localization, and confirmation of fluorescent BCAs through fluorescence imaging and PCR, are provided in this manuscript. This study features host‐plant interactions and subsequently biological and physiological mechanisms implicated in these interactions. The maximum time to complete all the steps of this protocol is approximately 3 months.

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Biological control of powdery mildew on Cornus florida using endophytic Bacillus thuringiensis

Cited by 16 publications

References 26 publications

Effect of Volatile Compounds Produced by Selected Bacterial Endophytes in Promoting Plant Growth

Effect of Volatile Compounds Produced by Selected Bacterial Endophytes in Promoting Plant Growth

Potential Biological Control Agents for Soilborne Fungal Pathogens in Tennessee Snap Bean Farms

Efficient fluorescence‐based localization technique for real‐time tracking endophytes route in host‐plants colonization

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