Biological control of the fungus gnats Bradysia difformis (Diptera, Mycetophilidae) in mushrooms with Heterorhabditis amazonensis in tropical conditions

San‐Blas, Ernesto; Luzardo, Mariangel; Larreal, Junior T.; Portillo, Edgar; Bastidas, Brynelly

doi:10.1016/j.scienta.2017.01.003

Cited by 11 publications

(7 citation statements)

References 26 publications

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“…EPN are natural inhabitants of the soil environment [ 17 , 18 , 19 , 20 , 21 ]. Their development cycle is associated with those of the host insects, whose bodies are used by EPN for the multiplication and subsequent emergence of up to several generations of nematodes.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Biological Activity of Field Isolates of Steinernema feltiae with a Commercial S. feltiae Biopesticide Product

Matuska-Łyżwa

Żarnowiec

Kaca

2021

Insects

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Insect trap studies were carried out to determine the presence of entomopathogenic nematodes (EPN) from the family Steinernematidae in the soils of Poland and to compare the biological activities of field nematode isolates with nematodes from commercial biopesticide. The fauna of these organisms in central Poland is poorly studied in both taxonomic and biological terms. Tilled soils representative of this region were sampled from cultivated fields. EPN were isolated from soil samples under laboratory conditions and identified using a key for species identification and molecular analysis. Basic morphometric parameters of infective juveniles and adult males of the first generation were determined. The research showed that males and infective juveniles Steinernema feltiae from Łoniów were the largest. The smallest infective juveniles were found in the isolate from Oblasy, and the smallest males in the isolate from Danków. In Poland, new field isolates showed close genetic similarity to other S. feltiae isolates. The research showed that the field isolates from Poland had greater infectivity and rate of reproduction compared with nematodes from the commercial biopesticide. The findings indicate the potential use of field S. feltiae isolates from Poland (iso1Lon, iso1Dan and iso1Obl) to develop new biopesticide products.

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Section: Introductionmentioning

confidence: 99%

Comparison of Biological Activity of Field Isolates of Steinernema feltiae with a Commercial S. feltiae Biopesticide Product

Matuska-Łyżwa

Żarnowiec

Kaca

2021

Insects

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“…Plant damage is caused by larvae that are responsible for causing damage to the roots, impairing the development of the root system and, consequently, interfering with the ability of plants to absorb water and nutrients, resulting in withering and stunted growth (San-blas et al 2017;Ye et al 2017). Likewise, injuries to the underground organs facilitate the entry of pathogens such as Pythium, Botrytis,…”

Section: Introductionmentioning

confidence: 99%

Toxicity of Bacillus sp. (Bacillales: Bacillaceae) on the fungus gnats, Bradysia aff. ocellaris larvae (Diptera: Sciaridae)

Duarte,

Martins

et al. 2023

Biosci. J.

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Bacillus thuringiensis (Bt) Berliner has potential for use in insect management. Its use can be an alternative for the management of Bradysia aff. ocellaris (Comstock), considered one of the main strawberry pests in a soilless system. Therefore, the objective of this work was to evaluate the toxicity of different bacteria on B. aff. ocellaris in laboratory and greenhouse bioassays. The following isolates were used in the experiments: Bacillus circulans (Bc), B. thuringiensis var. oswaldo cruzi (Bto) or B. thuringiensis var. israelensis (Bti) and B. thuringiensis var. kurstaki (Btk) In the laboratory, B. aff. ocellaris larvae showed high susceptibility to Bti isolate (92 % mortality) 14 days after treatment exposure (DAET). In contrast, the isolates Bc, Bto, and Btk showed less than 32 % mortality, not differing from the control treatment (water – 22 % mortality). According to the concentration-response curves the values of lethal concentration LC50 and LC90 were 4 x 106 CFU.mL-1 and 4 x 1015 CFU.mL-1. By applying Bti (4 × 1012 CFU.mL-1) at the base of strawberry plants growing in plastic pots containing commercial plant substrate, a reduction of 26 % in the emergence of B. aff. ocellaris was observed. According to these results, the Bti isolate is considered promising for the formulation of bioinsecticides based on Bt for the management of B. aff. ocellaris in strawberry culture.

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“…These small flies are considered the major insect pests of greenhouse production systems (CLOYD 2015) since they cause direct (larval feeding) and indirect damage through the spread of fungi propagules such as Fusarium, Phytium and Thielaviopsis (GARDINER et al, 1990;EL-HAMALAWI 2008). Its larvae feed on a wide-range of horticultural crops such as tomatoes, sweet peppers, lettuces, onions and ornamental flowers (SAN-BLAS et al, 2017), Eucalyptus nurseries (SANTOS et al, 2012) besides to be the main pest in mushroom crops, nurseries and greenhouse (MENZEL et al, 2003;Cloyd 2008) and adults can be a nuisance factor in Pinus timber yards (SCHÜHLI et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Bradysia aff. impatiens and Bradysia aff. ocellaris in the semi-hydroponic strawberry production system in Southern Brazil

Duarte

Cunha

2022

Cienc. Rural

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Soilless cultivation of strawberry is in expansion in Brazil due to factors like the reduction of phytosanitary problems and the possibility of extending the crop period, as well as the ergonometric facility for management of the culture. However, black fungus gnats (Sciaridae, Diptera) are ubiquitous pests in the greenhouse production system, causing direct and indirect damage to plants. ThIS study evaluated the occurrence of black fungus gnats species in semi-hydroponic cultivation of strawberry and to identify the species, as well as to describe the symptoms in infested plants. The research was developed in commercial productions of strawberry, in five municipalities (Arroio do Padre, Canguçu, Capão do Leão, Pelotas and Turuçu) during July to December 2017, where adult samples were collected twice a month, with petri dish traps containing water and neutral detergent placed inside the greenhouses between the plants. A total of 2030 adult gnats belonging to two species were collected. Bradysia aff. impatiens, accounted with 90.79% of the total, and Bradysia aff. ocellaris, accounted with 9.21%. In order to assist the correct determination of species, males of both species were identified and illustrated. With respect to the symptoms observed during the research, it was possible to verify since the wilted crown of plants to the death of plants, which were confirmed by the presence of larvae in the root system. As the occurrence of these insects is recent in strawberry, there is no pesticide registered in Brazil, so management strategies (cultural, physical, and sanitary) also are discussed.

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Biological control of the fungus gnats Bradysia difformis (Diptera, Mycetophilidae) in mushrooms with Heterorhabditis amazonensis in tropical conditions

Cited by 11 publications

References 26 publications

Comparison of Biological Activity of Field Isolates of Steinernema feltiae with a Commercial S. feltiae Biopesticide Product

Comparison of Biological Activity of Field Isolates of Steinernema feltiae with a Commercial S. feltiae Biopesticide Product

Toxicity of Bacillus sp. (Bacillales: Bacillaceae) on the fungus gnats, Bradysia aff. ocellaris larvae (Diptera: Sciaridae)

Bradysia aff. impatiens and Bradysia aff. ocellaris in the semi-hydroponic strawberry production system in Southern Brazil

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