Biological control potential of rhizosphere bacteria with ACC-deaminase activity against Fusarium culmorum in wheat

Abstract

Fusarium culmorum (W.G. Smith) is one of the fungal soil-borne plant pathogens causing significant yield and quality losses in cereals. Chemical attempts are not only insufficient for controlling such pathogens, but also they bring hazardous effects on the environment and living organisms. Therefore, environment-friendly plantbeneficial microorganisms including bacteria would replace chemical control agents as promising and sustainable pest management. Numerous studies showed that some strains of plant growth-promoting rhizobacteria comprising the enzyme ACC (1-aminocyclopropane-1-carboxylate)-deaminase could promote the plant growth acting as a biological control agent by lowering the level of excessive ethylene in plants exposed to biotic and abiotic stresses. This study was aimed to evaluate the rhizobacterial isolates obtained from rhizosphere of cereal plants in Konya and Karaman provinces in Turkey for potency of enzyme ACC-deaminase activity and in vitro/in vivo suppression ability on F. culmorum. In total 31 out of 463 rhizobacterial isolates successfully suppressed in vitro growth of F. culmorum on potato dextrose agar following dual-culture technique. Afterwards, the successful isolates were examined for ACC-deaminase activity using ACC as the sole nitrogen (N) source. Two isolates coded as Gu2 and 127b with the highest enzyme ACC-deaminase activity were included in pot trials under controlled conditions for assessing in vivo pathogen suppression ability on wheat seedlings. The in vivo pathogen suppression efficiency of Gu2 and 127b isolates was determined as 81.25% and 37.50%, respectively. It was determined that both rhizobacterial isolates belonged to Bacillus spp. with high reliable score based on MALDI Biotyper System classification results.

Determination of antagonistic potential of endophytic bacteria isolated from lettuce against lettuce white mould disease caused by Sclerotinia sclerotiorum

“…Biocontrol potential of epiphytic bacterial species of B. subtilis, B. amyloliquefaciens and B. pumilis, living in and around against lettuce white mould disease caused by Sclerotinia sclerotiorum roots of a wide range of crop plants, was tested against root-infecting soil-borne fungal disease agents such as Macrophomina phaseolina, Sclerotinia spp., Rhizoctonia solani and Fusarium spp. (Kamal et al, 2015;Abdeljalil et al, 2016;Imriz et al, 2020).…”

In vitro Suppression Effects of Rhizobacteria against Pseudomonas syringae pv. tomato the Agent of Bacterial Speck Disease of Tomato

“…Thirty-eight isolates were selected randomly from Rhizobacterial Culture Collection (Gul IMRIZ, Dicle University). The isolates were obtained from rhizosphere of different plants in Konya districts in previous study (Imriz et al, 2020). The properties belonging to the isolates including the codes, origin of plants, locations are given in Table 1.…”

Antifungal Properties of Coriandrum Sativum Extracts on Fusarium Spp. In Vitro

“…lycopersici causes tomato wilt, resulting in significant yield losses (Ramirez et al, 2020). In contrast, F. culmorum is able to infect a wide range of host plants, mainly cereals (Imriz et al, 2020) and produce mycotoxins, harmful to human (Rosello et al, 2015;Perczak et al, 2019). Increasing concern about the adverse effects of chemical fungicides resulted in an active search for alternative bioactive compounds from plants (Ben-Jabeur et al, 2015).…”