Biological Control Using the Ectoparasitoid,<i>Tamarixia radiata</i>, against the Asian Citrus Psyllid,<i>Diaphorina citri</i>, in the Lower Rio Grande Valley of Texas

Flores, Daniel; Ciomperlik, Matthew A.

doi:10.3958/059.042.0105

Cited by 26 publications

(16 citation statements)

References 5 publications

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“…Neste sentido, o uso do controle biológico através de inimigos naturais, como os parasitoides Tamarixia radiata (Waterson) (Hymenoptera: Eulophidae) (Parra et al, 2010;Flores & Ciomperlik, 2017) e Diaphorencyrtus aligarhensis (Shafee, Alam and Agarwald) (Hymenoptera: Encyrtidae) (Portalanza et al, 2017), seria uma boa alternativa de manejo, e tem sido feito pela liberação desses insetos em pomares abandonados, cemitérios e áreas não comerciais, como alternativa ao controle químico. Microrganismos entomopatogênicos também têm sido utilizados.…”

Section: /12unclassified

Desenvolvimento de estratégias alternativas visando ao controle do huanglongbing

Dorta¹,

Machado²,

Freitas-Astúa³

2019

Citrus Res. Technol.

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Section: /12unclassified

Desenvolvimento de estratégias alternativas visando ao controle do huanglongbing

Dorta¹,

Machado²,

Freitas-Astúa³

2019

Citrus Res. Technol.

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“…Hence, devising an alternative control strategy for ACP is very important and essential. Tamarixia radiata Waterston (Hymenoptera: Eulophidae) is the most effective natural enemy of ACPand it has been successfully used as a biological control agent of ACP in various countries [ 13 , 14 , 15 , 16 , 17 , 18 , 19 ]. Recently, several studies investigated the relationship between venomous proteins of the parasitoid wasp and its host insect prey [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Assessment of Candidate Reference Genes for Gene Expression Studies Using RT-qPCR in Tamarixia radiata, a Predominant Parasitoid of Diaphorina citri

Guo

Pan

Zhang

et al. 2020

Genes

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Tamarixia radiata (Waterston) is a predominant parasitoid of the Asian citrus psyllid (ACP), a destructive citrus pest and vector of huanglongbing (HLB) disease in the fields of southern China. To explore the functioning of target genes in T. radiata, the screening of specific reference genes is critical for carrying out the reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) under different experimental conditions. However, no reference gene(s) for T. radiata has yet been reported. Here, we selected seven housekeeping genes of T. radiate and evaluated their stability under the six conditions (developmental stage, sex, tissue, population, temperature, diet) by using RefFinder software, which contains four different programs (geNorm, ΔCt, BestKeeper, and NormFinder). Pairwise variation was analyzed by geNorm software to determine the optimal number of reference genes during the RT-qPCR analysis. The results reveal better reference genes for differing research foci: 18S and EF1A for the developmental stage; PRS18 and EF1A for sex, PRS18 and RPL13 for different tissues (head, thorax, abdomen); EF1A and ArgK between two populations; β-tubulin and EF1A for different temperatures (5, 15, 25, 35 °C); and ArgK and PRS18 for different feeding diets. Furthermore, when the two optimal and two most inappropriate reference genes were chosen in different temperatures and tissue treatments, respectively, the corresponding expression patterns of HSP70 (as the reporter gene) differed substantially. Our study provides, for the first time, a more comprehensive list of optimal reference genes from T. radiata for use in RT-qPCR analysis, which should prove beneficial for subsequent functional investigations of target gene(s) in this natural enemy of ACP.

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“…The proximity of abundant residential citrus to commercial groves in South Texas coupled with the frequent movement of D. citri adults between habitats (Sétamou, Sanchez, et al., ) facilitates grove recolonization by psyllids. Despite active releases of the nymphal parasitoid Tamarixia radiata for D. citri biological control (Flores & Ciomperlik, ), residential citrus trees remain largely unmanaged in South Texas (Arredondo, ) and continue to be a major source of psyllids for commercial groves (Sétamou & Alabi, ).…”

Section: Introductionmentioning

confidence: 99%

COPF: Citrus orchard perimeter fencing as a strategy for reducing Asian citrus psyllid (Hemiptera:Liviidae) infestation

Sétamou

Alabi²,

Tofangsazi

et al. 2018

J Applied Entomology

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Citrus grove colonization by the Asian citrus psyllid (Diaphorina citri) starts from border trees. This behaviour can be exploited by placing barriers along grove borders to prevent psyllid incursion and thwart population establishment and growth. We tested the effects of psyllid‐resistant mesh fencing along grove borders for season‐long control of D. citri populations. Deployment of sticky cards at different heights revealed that most D. citri (>99%) were collected on traps placed at or below 3.7 m along grove border. Thus, psyllid‐resistant mesh fencing (3.7 m in height) was installed along the eastern and southern borders of a 3‐year‐old grapefruit grove to protect against D. citri infestations, while the west and north edges were left unprotected. A similar adjacent grove with no border fencing served as untreated control. A reduction of 55%–98% of D. citri numbers was observed in the grove with border fences relative to the untreated control. D. citri density reductions were more pronounced on the sides with border fences suggesting that the observed effects were due to the mesh fencing. Thus, border mesh fencing as a standalone approach or coupled with other management strategies, such as border sprays, could be an effective psyllid management strategy. The season‐long benefits of border fences are not limited to D. citri population control, but also significant reductions of insecticide input and nontarget effects, thus substantially mitigating the risks of secondary pest outbreaks and ensuring the sustainability of D. citri management in citrus groves.

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Biological Control Using the Ectoparasitoid,Tamarixia radiata, against the Asian Citrus Psyllid,Diaphorina citri, in the Lower Rio Grande Valley of Texas

Cited by 26 publications

References 5 publications

Desenvolvimento de estratégias alternativas visando ao controle do huanglongbing

Desenvolvimento de estratégias alternativas visando ao controle do huanglongbing

Comprehensive Assessment of Candidate Reference Genes for Gene Expression Studies Using RT-qPCR in Tamarixia radiata, a Predominant Parasitoid of Diaphorina citri

COPF: Citrus orchard perimeter fencing as a strategy for reducing Asian citrus psyllid (Hemiptera:Liviidae) infestation

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