Biological conversion of methane to methanol through genetic reassembly of native catalytic domains

Power-to-liquidviasynthesis of methanol, DME or Fischer–Tropsch-fuels: a review

“…Hereby the use of bio membranereactors and biocatalysts (enzymes) can be differentiated. 68 For example, biological conversion of methane to methanol, 69,70 the synthesis of petroleum-like hydrocarbons 71 or biofuels from microalgae 72 could be achieved. Currently, there are several challenges that need to be addressed, such as dissolving CO 2 in or poisoning of the used solution.…”

Towards a unified understanding of the copper sites in particulate methane monooxygenase: an X-ray absorption spectroscopic investigation

“…As exemplied by the 20Z-pMMO EXAFS study and despite the increasing evidence that pMMO contains only mononuclear copper sites, researchers in this eld have been unable to account for the fact that EXAFS analyses of multiple pMMO samples and recombinant constructs, performed by multiple investigators working independently, consistently measured a short distance ($2.5-2.7Å) Cu-Cu scattering interaction. 15,16,[18][19][20][24][25][26][27] In order to advance the mechanistic understanding of the enzyme, the previous EXAFS studies must collectively be reconciled with themselves and with the growing body of experimental evidence indicating exclusively monocopper centers in pMMO. Herein, we rst revisit the Cu XAS of pMMO to understand how various samples could have very similar XAS spectra and yet different copper oxidation state distributions based on EPR.…”

Biochemical evidence of both copper chelation and oxygenase activity at the histidine brace

“…In our experience 5 , 6 , it is very difficult to biochemically or structurally determine if a novel Cu-protein is an enzyme or a Cu-chelator. The ongoing scientific controversy regarding the active site of pMMO 7 – 9 is reflecting the same problem. Absence of activity in metallo-enzyme assays can in principle be due to a number of factors including lack of suitable reducing agent or substrate.…”