Biological Decolourization of Textile and Paper Effluents by Pleurotus florida and Agaricus bisporus (White-rot Basidiomycetes)

Abstract

The extracellular ligninolytic enzymes of white-rot fungi are thought to catalyse the initial steps during the degradation of highly complex compounds like lignin or polycyclic aromatic hydrocarbons. We studied the ability of Pleurotus florida isolated from the foothills of the Western Ghats, India to decolourize the three dyestuffs, Reactive Green, Yellow and Blue, which are widely used in the textile industry around Coimbatore, Tamil Nadu, India. The crude culture filtrate of Pleurotus florida when incubated with different concentrations of dye decolourized it efficiently on the third day. The highest colour removal was found in the case of Reactive Blue. However, when Agaricus bisporus extract was supplemented with Pleurotus florida filtrate, the efficiency increased. The dye decolourization was advanced to the second day and the efficiency of dye decolourization of Reactive Yellow was 89% followed by Reactive Green, which was 45% when a dye concentration of 0.5% was used. Pleurotus florida filtrate alone and in combination with Agaricus bisporus extract reduced the aromatic compounds in textile and paper industry effluents on the first day with >90% efficiency.

Ligninolytic enzymes: a biotechnological alternative for bioethanol production

“…Therefore, laccases have been employed in several areas such as bioremediation of aromatic recalcitrant compounds [18], treatment of effluents polluted with lignin [19], chemical synthesis [20,21], degradation of a wide number of textile dyes [22][23][24][25], and biomass pretreatment for biofuel production [26][27][28].…”

Decolorization of synthetic brilliant green carpet industry dye through fungal co-culture technology

“…The literature evidences number of white-rot fungi producing the lignin-degrading enzymes, lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (Fu and Viraraghavan, 2001) which can degrade dyes. Manganese peroxidase (MnP) and lignin peroxidase (LiP) reported as the main enzyme involved in dye decolorization by Phanerochaete chrysosporium (Kirby et al, 1995;Chagas and Durrant, 2001), Pleurotus florida and Agaricus bisporus (Shanmugam et al, 2005). Involvement of enzymatic system of basidiomycetes has a great efficiency for dye bioremediation process.…”

Synthetic dye decolourization, textile dye and paper industrial effluent treatment using white rot fungi Lentines edodes

“…The treatment was carried out using crude laccase and a consortium of microorganisms that produces dye-degrading enzymes. A similar work reported using Pleurotus florida and Agaricus bisporus crude filtrates for decolourization of reactive dyes, revealed an efficient decolourization of Reactive Yellow of 89% followed by Reactive Green and reactive blue [19]. The efficiency of the crude extract to decolourize the dyes and effluents was monitored by change in their respective absorbance maxima every alternate day after the addition of dye/effluent for a period of 6 days.…”

“…Another fungus, Agaricus bisporus showed a greater extent of tyrosinase activity (1.6 U/ml), which plays an important role in the oxidation of phenolic compounds [16,17], Downloaded by [Yale School of Medicine] at 07:36 22 July 2015 was also selected for the study. All these enzymes have been implicated in dye decolourization as well as for effluent treatment [18,19]. In the study, the crude filtrate of Lentines edodes decolourized the reactive blue dye (77.5%) to a greater extent on the third day of incubation than the other dyes ( Table 1).…”

“…Preliminary results have shown that laccases are able to graft various phenolics acid derivatives onto kraft pulp fibers [9,10]. Shanmugam et al [19] has reported that the crude extract of laccase from Pleurotus florida possessed an effective dye, textile and paper effluents decolourization. In the light of the aforementioned studies, this study was designed to investigate the effect of Lentines edodes obtained from Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India was used for decolourization of textile dye-containing wastewater and paper industry effluent.…”