“…The stability of this process and the treatment outcome depend on the microbial community structure, the amount of functional groups belong to the activated sludge (denitrifiers, nitrifiers, heterotrophic microorganisms), their activity (Nitrate Utilization Rate-NUR, Ammonia Utilization Rate-AUR, Oxygen Utilization Rate-OUR), and the ratio of AOB (Ammonia-Oxidizing Bacteria) to NOB (Nitrite-Oxidizing Bacteria) [14,16,17], while the microbial activity is influenced by operating parameters, such as pH, temperature, and oxygen concentration as well as the nitrogen and COD loading rate and COD/TN ratio in the influent [1,13,15,18]. The increase in biological nitrogen removal efficiency in wastewater with a low COD/TN ratio can be achieved by adding an external carbon source [18]. The most frequently used carbon sources are easily biodegradable organics, such as ethanol [19,20], methanol [20][21][22] and acetic acid [15,20,21,23].…”