Biological denitrification inhibition (BDI) in the field: A strategy to improve plant nutrition and growth

Galland, William; Piola, Florence; Burlet, Alexandre; Mathieu, Céline; Nardy, Mélisande; Poussineau, Sophie; Blazère, Leslie; Gervaix, Jonathan; Puijalon, Sara; Simon, Laurent; Haichar, Feth el Zahar

doi:10.1016/j.soilbio.2019.06.009

Cited by 37 publications

(35 citation statements)

References 46 publications

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“…The amount of DNA extracted was then estimated using the Quant-iT PicoGreen®double-stranded DNA (dsDNA) Assay kit (Molecular Probes, Carlsbad, CA 92008, USA). Total bacteria and denitrifier abundances were quantified by quantitative PCR using primers targeting the 16S ribosomal RNA (rRNA) and nirK/nirS genes, respectively, as described previously by Galland et al [27].…”

Section: Quantification Of Total Bacteria and Denitrifier Abundancementioning

confidence: 99%

“…Nitrate was extracted from 5 g eq. of dried soil according to Galland et al [27]. Briefly, nitrate was extracted from 5 g eq.…”

Section: Nitrate Concentrations In Soilmentioning

confidence: 99%

“…Total N concentration was measured using an elemental analyzer (FlashEA 1112, Thermo Scientific, Waltham, MA, USA) from three leaves and three root systems per plot (nine per treatment), using 2 mg of ground root or leaf material [27]. Leaf and root N content was expressed as a percentage of N in leaf and root dry mass.…”

Section: Plant N Contentmentioning

confidence: 99%

“…Recently, Galland et al [27] demonstrated that an exogenous supply of procyanidins in a field experiment involving lettuce crops induced BDI, which resulted in (i) a decrease in denitrification activity, (ii) an increase in nitrate concentration in plant tissues, and (iii) a significant increase in lettuce mass, compared to lettuces not amended with procyanidins. Moreover, lettuce (Lactuca sativa) tested by Galland et al [27] is a crop that lasts only a few weeks and requires only one application of fertilizers [28] to allow the crop to reach term at acceptable and marketable yields. Other plants species such as celery (Apium graveolens L.) have a longer growing cycle than lettuce and require more nitrogen for their growth.…”

Section: Introductionmentioning

confidence: 99%

“…Unplanted plots and those planted with celery but not amended with either fertilizers or procyanidins were used as controls. Procyanidin concentrations were selected from the most effective concentration evidenced by Galland et al [27], which was approximately 210 kg•ha −1 of procyanidins.…”

Section: Introductionmentioning

confidence: 99%

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Does Biological Denitrification Inhibition (BDI) in the Field Induce an Increase in Plant Growth and Nutrition in Apium graveolens L. Grown for a Long Period?

Galland

Piola

Mathieu³

et al. 2020

Microorganisms

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Intensive agriculture uses a lot of nitrogen fertilizers to increase crop productivity. These crops are in competition with soil-denitrifying microorganisms that assimilate nitrogen in the form of nitrate and transform it into N2O, a greenhouse gas, or N2. However, certain plant species exude secondary metabolites, called procyanidins, which inhibit denitrifiers and increase the nitrate pool in the soil available for plant nutrition. This phenomenon is called biological denitrification inhibition. Previously, we showed that the addition of exogenous procyanidins to a lettuce crop induces denitrifier inhibition and increases nitrate content in the soil, affecting lettuce morphological traits. Here, the effects of procyanidin amendments in the field on a more long-term and nitrogen-consuming crop species such as celery were tested. The effects of procyanidin amendment on celery growth with those of conventional ammonium nitrate amendments were, therefore, compared. Denitrification activity, nitrate concentration, the abundance of denitrifying bacteria in the soil, and traits related to celery growth were measured. It was shown that the addition of procyanidins inhibits denitrifiers and increases the soil nitrate level, inducing an improvement in celery morphological traits. In addition, procyanidin amendment induces the lowest nitrogen concentration in tissues and reduces N2O emissions.

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Section: Quantification Of Total Bacteria and Denitrifier Abundancementioning

confidence: 99%

“…Nitrate was extracted from 5 g eq. of dried soil according to Galland et al [27]. Briefly, nitrate was extracted from 5 g eq.…”

Section: Nitrate Concentrations In Soilmentioning

confidence: 99%

Section: Plant N Contentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Does Biological Denitrification Inhibition (BDI) in the Field Induce an Increase in Plant Growth and Nutrition in Apium graveolens L. Grown for a Long Period?

Galland

Piola

Mathieu³

et al. 2020

Microorganisms

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Rhizosphere soil bacterial communities and nitrogen cycling affected by deciduous and evergreen tree species

Liu

Wang

Liu

et al. 2022

Ecology and Evolution

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Deciduous and evergreen trees differ in their responses to drought and nitrogen (N) demand. Whether or not these functional types affect the role of the bacterial community in the N cycle during drought remains uncertain. Two deciduous tree species (Alnus cremastogyne, an N2‐fixing species, and Liquidambar formosana) and two evergreen trees (Cunninghamia lanceolata and Pinus massoniana) were used to assess factors in controlling rhizosphere soil bacterial community and N cycling functions. Photosynthetic rates and biomass production of plants, 16S rRNA sequencing and N‐cycling‐related genes of rhizosphere soil were measured. The relative abundance of the phyla Actinobacteria and Firmicutes was higher, and that of Proteobacteria, Acidobacteria, and Gemmatimondaetes was lower in rhizosphere soil of deciduous trees than that of evergreen. Beta‐diversity of bacterial community also significantly differed between the two types of trees. Deciduous trees showed significantly higher net photosynthetic rates and biomass production than evergreen species both at well water condition and short‐term drought. Root biomass was the most important factor in driving soil bacterial community and N‐cycling functions than total biomass and aboveground biomass. Furthermore, 44 bacteria genera with a decreasing response and 46 taxa showed an increased response along the root biomass gradient. Regarding N‐cycle‐related functional genes, copy numbers of ammonia‐oxidizing bacteria (AOB) and autotrophic ammonia‐oxidizing archaea (AOA), N2 fixation gene (nifH), and denitrification genes (nirK, nirS) were significantly higher in the soil of deciduous trees than in that of the evergreen. Structural equation models explained 50.2%, 47.6%, 48.6%, 49.4%, and 37.3% of the variability in copy numbers of nifH, AOB, AOA, nirK, and nirS, respectively, and revealed that root biomass had significant positive effects on copy numbers of all N‐cycle functional genes. In conclusion, root biomass played key roles in affecting bacterial community structure and soil N cycling. Our findings have important implications for our understanding of plants control over bacterial community and N‐cycling function in artificial forest ecosystems.

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Procyanidin inhibited N2O emissions from paddy soils by affecting nitrate reductase activity and nirS- and nirK-denitrifier populations

Yin

Fan

et al. 2021

Biol Fertil Soils

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Biological denitrification inhibition (BDI) in the field: A strategy to improve plant nutrition and growth

Cited by 37 publications

References 46 publications

Does Biological Denitrification Inhibition (BDI) in the Field Induce an Increase in Plant Growth and Nutrition in Apium graveolens L. Grown for a Long Period?

Does Biological Denitrification Inhibition (BDI) in the Field Induce an Increase in Plant Growth and Nutrition in Apium graveolens L. Grown for a Long Period?

Rhizosphere soil bacterial communities and nitrogen cycling affected by deciduous and evergreen tree species

Procyanidin inhibited N2O emissions from paddy soils by affecting nitrate reductase activity and nirS- and nirK-denitrifier populations

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