Biological detoxification of different hemicellulosic hydrolysates using Issatchenkia occidentalis CCTCC M 206097 yeast

Abstract

This work had as its main objective to contribute to the development of a biological detoxification of hemicellulose hydrolysates obtained from different biomass plants using Issatchenkia occidentalis CCTCC M 206097 yeast. Tests with hemicellulosic hydrolysate of sugarcane bagasse in different concentrations were carried out to evaluate the influence of the hydrolysate concentration on the inhibitory compounds removal from the sugarcane bagasse hydrolysate, without reduction of sugar concentration. The highest reduction values of inhibitors concentration and less sugar losses were observed when the fivefold concentrated hydrolysate was treated by the evaluated yeast. In these experiments it was found that the high sugar concentrations favored lower sugar consumption by the yeast. The highest concentration reduction of syringaldehyde (66.67%), ferulic acid (73.33%), furfural (62%), and 5-HMF (85%) was observed when the concentrated hydrolysate was detoxified by using this yeast strain after 24 h of experimentation. The results obtained in this work showed the potential of the yeast Issatchenkia occidentalis CCTCC M 206097 as detoxification agent of hemicellulosic hydrolysate of different biomass plants.

Screening of Yeasts for Selection of Potential Strains and Their Utilization for In Situ Microbial Detoxification (ISMD) of Sugarcane Bagasse Hemicellulosic Hydrolysate

“…Detoxification results of hdrolysates by four strains i.e. P. occidentalis M1, Y1 0 a, Y1 0 b and Y3 0 corroborates with the work of Fonesca et al [21].…”

“…It is important to note that 5-HMF and furfural removal are not only related to the type of yeast used in the process, but also the kind of lignocelullosic material used to obtain the hemicellulosic hydrolysates. The different hemicellulosic hydrolysates provide different percentages removal by P. occidentalis [21]. This study demonstrates the potential of the yeasts for removal of inhibitors under the experimental conditions, pH (4.0 or 5.0) and agitation rate (100 rpm or 300 rpm), employing microbial detoxification approach.…”

“…Likewise, Fonseca et al [21] employed the same yeast strain P. occidentalis CCTCC M Table 4 Initial and final concentrations of toxic compounds in hemicellulose hydrolyzate treated biologically by Picchia occidentalis 206097 and reported 6.1 % removal of 3.3 g/l acetic acid, 100 % of 0.02 g/l of HMF and 100 % of 0.16 g/l furfural, after 72 h of biological detoxification. These results showed that P. occidentalis is a promising candidate for the in situ removal of toxic compounds from hemicellulosic hydrolysate.…”

Evaluation of microorganisms performance in hemicellulosic hydrolyzate detoxification and sugarcane bagasse lignin removal for ethanol production

“…syringaldehyde, 4-hydrozibenzaldehyd and vanillin are formed from the degradation of the lignin molecular structure during the lignocellulosic biomass pretreatment (FONSECA et al, 2011)..…”

“…Depending on the concentration, weak acids, furanic compounds and phenolic compounds are toxic to fermenting microorganisms, inhibiting their metabolism LARSSON et al, 1999), so that they must be removed from the hemicellulosic hydrolyzate prior the fermentation step. In this case, a detoxification step is essential to overcome the metabolism of sugars by the fermenting microorganism (FONSECA et al, 2011(FONSECA et al, , 2013YU et al, 2011).…”