Biological Effect of Erytropoetine in Different Concentrations on the Cell Culture

Maksymenko, O. P.; Shkuropat, A.; Hasiuk, O.

doi:10.26693/jmbs02.04.185

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“…Erythropoietin is a hormone that regulates not only the formation of erythrocytes, but also has other pronounced pleiotropic effects. Thus, it was shown that erythropoietin caused a decrease in the activity of ALT, TNF-α and MDA in rats with hepatic ischemia, i.e.it had a hepatoprotective effect [15]. In the study [10], it was found that erythropoietin at medium and high concentrations inhibited the division of mouse fibroblasts in vitro, but at all concentrations it contributed to faster cell adaptation.…”

Section: Introductionmentioning

confidence: 99%

Influence of biologically active substances on synthesis function and cellular destruction of hepatocytes in vitro

Shkuropat¹,

Shvets²,

Golovchenko³

et al. 2022

Fiziol Zh

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The aim of the study was to reveal the effect of biologically active substances on the synthesis activity and cellular destruction of hepatocytes in vitro. Liver sections were prepared for investigation and placed in culture vials with DMEM nutrient medium with 15% calf serum, glucose, and antibiotics (streptomycin and penicillin). Liver sections were incubated for 14 days with interleukin-2 (roncoleukin) at a concentration of 5000 IU/ml and 7500 IU/ml, and erythropoietin (epobiocrine, “Biopharma”, USA) at a concentration of 13 IU/ml (high concentration), 6.5 IU/ml (medium concentration) and 1.3 IU/ml (low concentration) and without stimulation (control cultures). Synthesis activity and cellular destruction of hepatocytes were studied by determining the protein content, alanine aminotransferase and aspartate aminotransferase activity in the supernatant of liver organ cultures on the 7th and 14th days of incubation. It was found that culturing organotypic cultures with IL-2 did not affect the synthesis function of hepatocytes, but reduced aspartate aminotransferase activity throughout the culture period. At a concentration of 7500 IU/ml IL-2 showed a weak hepatotoxic effect. It was found that erythropoietin at a medium concentration had a hepatoprotective effect, at a high concentration it suppressed the synthesis activity of hepatocytes and contributed to the destruction of the cytoplasmic membrane of cells. At low concentrations, erythropoietin increased the synthesis activity of liver cells but caused an increase in the activity of aminotransferases, this may indicate both mass cell death and intensification of amino acid transamination processes. It was established that interleukin and its inhibitor cause biological effects when incubated with organotypic cultures.

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Section: Introductionmentioning

confidence: 99%