Biological Effect of Gamma Rays According to Exposure Time on Germination and Plant Growth in Wheat

Hong, Min Jeong; Kim, Dae Yeon; Jo, Yeong Deuk; Choi, Hong‐Il; Ahn, Joon-Woo; Kwon, Soon‐Jae; Kim, Sang Hoon; Seo, Yong Weon; Kim, Jin Baek

doi:10.3390/app12063208

Cited by 34 publications

(26 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In their study on the dose-and time-dependent effects of γ-irradiation on germination rate, plant growth, chlorophyll content, oxidative stress (OS), gene expression, and antioxidant activity changes, Hong et al [ 60 ] reported that long-term exposure to γ-rays had a significantly negative effect on the seed germination percentage. Also, they observed no differences in the final germination percentage between control and irradiated seeds.…”

Section: Discussionmentioning

confidence: 99%

Influence of gamma radiation and phenylalanine on secondary metabolites in callus cultures of milk thistle (Silybum marianum L.)

Khalifa

Abd-ElShafy

Abu-Khudir

et al. 2022

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

Background A useful technique for growing large amounts of plant material is in vitro propagation of important medicinal plants. The present investigation deals with the enhancement of secondary metabolite production via elicitation using gamma (γ)-radiation and phenylalanine (Phe) precursor feeding in callus cultures of Silybum marianum L. Results Seeds were exposed to two doses of γ-radiation (25 and 50 Gy) and the calli derived from stem explants obtained from seedlings of these radiated seeds were treated with different concentrations of Phe. The biosynthesis of phenols and flavonoids was evaluated. It was found that callus cultures derived from explants of the seeds exposed to 25 Gy γ-radiation and treated with 4 mg/l Phe accumulated the maximum phenolic content (34.27±0.02 mg/g d.wt.), while the highest flavonoid content (9.56±0.12 mg/g d.wt.) was found in callus cultures derived from explants of seeds radiated with 25 Gy γ-radiation and subjected to 1 mg/l Phe. Similarly, HPLC quantification revealed that the production of flavonoids was highly accumulated (1343.06 μg/mg d.wt.) in callus cultures from explants of seeds exposed to 25 Gy γ-radiation and grown at 1 mg/l Phe compared to the other treatments. In addition, a total of 11 important flavonoids have been determined in all callus cultures, except for acacetin-7-O-rutinoside, which was not found in the callus culture of the control. Conclusions These findings suggest that γ-radiation combined with Phe can improve the metabolism of S. marianum L. and could be used to produce such valuable metabolites on a commercial scale.

show abstract

Section: Discussionmentioning

confidence: 99%

Influence of gamma radiation and phenylalanine on secondary metabolites in callus cultures of milk thistle (Silybum marianum L.)

Khalifa

Abd-ElShafy

Abu-Khudir

et al. 2022

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

show abstract

“…The use of gamma radiation on grain has been debatable. Some argue that this type of ionizing radiation can induce mutations being effectively beneficial in plant breeding [ 19 ]. Some suggests gamma radiation caused high mortality and being harmful for germination [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Utilizing Proteomic Approach to Analyze Potential Antioxidant Proteins in Plant against Irradiation

Yang

et al. 2022

Antioxidants

View full text Add to dashboard Cite

Gamma-ray irradiation is an effective and clean method of sterilization by inactivating microorganisms. It can also be applied to induce anti-oxidants for future application. In this study, the mung bean (Vigna radiata) was exposed to gamma-ray irradiation under the dose of 0, 5 or 10 kGy. With increasing irradiation doses, the concentrations of malondiadehyde decreased while the levels of total flavonoids and DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity increased. It has been shown that consuming flavonoids can provide protective effects. In addition, proteomic analysis identified several proteins having anti-oxidant activities in the 5 kGy irradiated group. These proteins are Apocytochrome f, Systemin receptor SR 160, DELLA protein DWARF8, DEAD-box ATP-dependent RNA helicase 9, ζ-carotene desaturase (ZDS), and Floral homeotic protein AGAMOUS. Our findings indicate that plants contain a variety of phytochemicals and antioxidant proteins which may effectively prevent oxidative stress caused by irradiated peroxidation.

show abstract

“…An adaptive response was first demonstrated using mutations in bacteria, and later the same phenomenon was identified in other objects. It is especially characteristic of physical mutagens (Holme et al, 2019;Hong et al, 2022). Although there are hypotheses about the mechanisms of this phenomenon, there is no definitive justification for this effect (Khursheed et al, 2015;Palmieri et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Occurrence of cytogenetic effects under the action of epimutagen in winter wheat

Beiko

Nazarenko

2022

Regul. Mech. Biosyst.

View full text Add to dashboard Cite

Research on studying the features of 0.01%, 0.05%, 0.10% and 0.50% epimutagen Triton-X-305 impact at the cellular level, which means the way of identifying the viability of pollen in plants of the first generation, and cytological analysis of chromosomal aberrations in the cells of the primary meristem of germinal roots during germination of bread winter wheat seeds have been conducted. Research in this direction allows us to significantly improve the monitoring of same type of substances in the environment and to forecast the nature of their action at the DNA-level. Genotypes Podolyanka and Spivanka created by Ukrainian selection and varieties of French selection Altigo, Courtot, Lyrik, Flamenko have been studied. These genotypes were selected in order to characterize possible genotype-mutagenic interactions for a compound of complex hereditary pattern for a range of concentrations with maximum contrast taking into account the possible high site-specific effect. The main purpose of this research was to identify the specificity of impact of the agent Triton X-305 at the cellular level and identify parameters fully showing the effect of this substance on the subsequent induction of biodiversity and the enhancement of hereditary variability at the cellular level. Such indicators as pollen sterility effected by various concentrations, the total frequency of chromosomal rearrangements, the spectrum of chromosomal rearrangements, including fragments and double fragments, single and double bridges, micronuclei and lagging chromosomes have been investigated. The ratio of fragments to bridges as an indicator of the active factor nature, the number of cells with two or more rearrangements as an indicator of genetic toxicity of this substance has been established. As a result, a significantly weaker effect of the epimutagen on the overall frequency of chromosomal rearrangement has been shown unlike other factors of a mutagenic nature, at the same time changing the ratio of the obtained aberrations in favour of micronuclei and lagging chromosomes. In addition, other parameters are determined as more meaningful for identifying a specific agent of impact, and the differences in concentrations are less contrasting. There is also a less significantly decreasing fertility, however, this parameter is highly dependent on the source material. In the future, we intend to assess the variability, primarily of a hereditary nature, by the way of a visual analysis of the obtained material in subsequent generations, as well as through biochemical analysis, yield qualities analysis and morphometry of the obtained material.

show abstract

Biological Effect of Gamma Rays According to Exposure Time on Germination and Plant Growth in Wheat

Cited by 34 publications

References 54 publications

Influence of gamma radiation and phenylalanine on secondary metabolites in callus cultures of milk thistle (Silybum marianum L.)

Influence of gamma radiation and phenylalanine on secondary metabolites in callus cultures of milk thistle (Silybum marianum L.)

Utilizing Proteomic Approach to Analyze Potential Antioxidant Proteins in Plant against Irradiation

Occurrence of cytogenetic effects under the action of epimutagen in winter wheat

Contact Info

Product

Resources

About