Biological effect of He‐Ne laser: Investigations on functional and micromorphological alterations of cell membranes, in vitro

Abstract

A 1-J/cm2 dose of single laser-irradiation applied on primary human embryo fibroblast culture was not followed either by functional or micromorphological alterations of cell surfaces. This dose, however, applied four times with 24-hour intervals changed the functional conditions as well as surface charges of cell membranes. As detection methods, radioactive glucosamine uptake, lectin, and cationized ferritin-binding techniques were applied. At the same time, the scanning and transmission electron microscopy of laser-irradiated cells did not reveal any micromorphological or ultrastructural alterations. The effect of a 5-J/cm2 dose did not differ from that of 1 J/cm2. It is suggested that laser radiation-induced circumstances on cell surfaces can contribute to the strength of cell-to-cell contacts, i.e., to the stimulation of epithelization experienced in the clinical gynecologic practice.

He-Ne laser irradiation affects proliferation of cultured rat Schwann cells in a dose-dependent manner

“…Other low energy laser irradiation experiments on cell cultures in vitro have been performed mainly on fibroblasts (Bosatra et al, 1984;Kubasova et al, 1984;Balboni et al, 1986;Boulton & Marshall, 1986;Lam et al, 1986;Glassberg et al, 1988;Pourreau-Schneider et aI., 1990), but also on HeLa cells (Karu et al, 1984), E. coli (Karu et aI., 1983), and lymphocytes (Passarella et al, 1985;Meyers et al, 1987;Fedoseyeva et al, 1988;Inuoe et aI., 1989;Manteifel et al, 1991). In most of these experiments similar modulatory effects of laser irradiation on cell proliferation were seen.…”

The Effects of Helium-Neon Laser upon Regeneration of the Crushed Peroneal Nerve

“…ported in Wobarsht, demonstrated accelerated growth of somatic nerves with exposure to He-Ne laser radiation (1 7). Kubasova et al (6) found that a 1-J/cm2 dose of He-Ne laser radiation applied four times at 24-hour intervals changed the firnctional conditions as well as surface charges of embryonic cell membranes, which is consistent with stimulation of epithelization (6). Walker and Akhan-jee (1 5 ) , as a result of their study on laser-induced soniatosensory evoked potentials, believe the peripheral nervous system possesses a previously unsirspected degree of photosensitivity, which may provide rationale for therapeutic applications of lowpower laser.…”

Efficacy of low power laser therapy and exercise on pain and functions in chronic low back pain

“…Laser irradiation has a demonstrable effect in vitro on both metabolism and surface charges on cells in culture, but the ultra-structure is unchanged [40]. Research studies of the effects of low power laser irradiation on biological functioning, are growing in number and scope.…”