Biological Effects Induced by 68Ga-Conjugated Peptides in Human and Rodent Tumor Cell Lines

EVALUATION OF THE CYTOTOXIC EFFECTS INDUCED BY 68Ga-NODAGA-c(RGDfK) AND 68Ga-DOTA-c(RGDfK) IN MURINE MALIGNANT MELANOMA

“…In the present study an insignificant increase in total thiol in tissues might be due to increased free radical production, but in amounts that are scavenged by antioxidants, such that the damage is limited to few protein molecules. Our results are consistent with those obtained in other experimental studies, performed in vitro, on B16/F10 melanoma cell line [9].…”

“…In the previous experimental study performed this time in vitro on B16/F10 melanoma cell line [9], we investigated whether alpha(v)beta(3) integrin receptor binding 68 Ga-labelled RGD peptides synthesized for PET diagnostic applications could influence growth of tumour cells as a result of apoptosis promoting and/or cell cycle dysregulation. Briefly, we investigated in what extent the effects of 68 Ga-labelled peptides were due to induction of apoptosis or cell cycle arrest in tumour cells in connection with oxidative stress generation.…”

“…In our previous study we focused on the biological effects induced in vitro by different 68 Ga-radiolabelled peptides developed for PET investigation on several human and murine tumour cell lines [9]. In this paper, we expanded our research in vivo exploring the biological effects of two selected 68 Ga-peptide conjugates employing distinct chelators (DOTA-RGD versus NODAGA-RGD) after their intravenous administration in B16/F10 melanomabearing mice.…”