Biological effects of a rat monoclonal anti-mouse IFN-γ antibody produced by in vitro immunization

Echtenacher, Bernd; Hederer, Rosemarie; Krammer, Peter H.

doi:10.1016/s0171-2985(87)80103-1

Search citation statements

Order By: Relevance

Paper Sections

Select...

Discussion1

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1989

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

(1 citation statement)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To establish such a sensitive assay, we first tried to produce a monoclonal antibody specific against G-CSF. As a few monoclonal antibodies to cytokines are produced using recombinant antigens for immunization [1,7,20], we used rG-CSF as an antigen.…”

Section: Discussionmentioning

confidence: 99%

Measurement of human G-CSF by enzyme-linked immunosorbent assay using monoclonal antibody

et al. 1989

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Measurement of human G-CSF by enzyme-linked immunosorbent assay using monoclonal antibody

et al. 1989

View full text Add to dashboard Cite

Measurement of human granulocyte-macrophage colony-stimulating factor (GM-CSF) by enzyme-linked immunosorbent assay

et al. 1989

View full text Add to dashboard Cite

An IgG monoclonal antibody against recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF), designated HGM1, was produced by fusion of immune mouse splenocytes with HAT-sensitive murine myeloma cells. A sandwich enzyme-linked immunosorbent assay (ELISA) for measurement of human GM-CSF was developed using this HGM1 and a polyclonal antibody against GM-CSF raised in a rabbit. GM-CSF in culture supernatants of phytohemagglutinin (PHA)- or concanavalin A (Con A)-stimulated peripheral blood mononuclear cells (PBMC) were measured by this ELISA system and the conventional CFU-GM colony formation method. The data indicated that the ELISA was highly efficient and sensitive for the detection of as little as 50 pg/ml recombinant GM-CSF. The CFU-GM colony assay may be influenced by other cytokines which can enhance or suppress colony formation, and ELISA for GM-CSF is more useful for kinetic studies of precise levels of production from PBMC.

show abstract

Biological effects of a rat monoclonal anti-mouse IFN-γ antibody produced by in vitro immunization

Cited by 2 publications

References 26 publications

Measurement of human G-CSF by enzyme-linked immunosorbent assay using monoclonal antibody

Measurement of human G-CSF by enzyme-linked immunosorbent assay using monoclonal antibody

Measurement of human granulocyte-macrophage colony-stimulating factor (GM-CSF) by enzyme-linked immunosorbent assay

Contact Info

Product

Resources

About