Biological effects of acoustic cavitation

Carstensen, E. L.

doi:10.1016/0301-5629(86)90287-5

Cited by 18 publications

(2 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, the difference in the acoustic impedance of conduit materials was another important factor that caused foreign body reactions. Carstensen39 and Holland and colleagues40 found a potential hazard in the form of collapse cavitations as a result of the standing waves generated from the interference of ultrasound waves reflected at the high‐impedance interface (e.g., at the mineralized matrix of the bone) 39, 40. Tissue inflammation may have been arisen from the events associated with this phenomenon, such as the formation of free radicals, high transient intensity of acoustic pressures, and high local temperature.…”

Section: Discussionmentioning

confidence: 99%

Low‐intensity‐ultrasound–accelerated nerve regeneration using cell‐seeded poly(D,L‐lactic acid‐co‐glycolic acid) conduits: An in vivo and in vitro study

et al. 2005

View full text Add to dashboard Cite

This study investigated the effects of low intensity ultrasound on seeded Schwann cells within poly(DL-lactic acid-co-glycolic acid) (PLGA) conduits by in vitro and in vivo trials for peripheral nerve regeneration. The possible differences in the ultrasonic effects when using biodegradable and non-biodegradable materials as the conduits were also studied, using silicone rubber tubes as comparisons. In the in vitro study, seeded Schwann cells were cultured in serum deprivation culture medium that simulated the environment of mechanical trauma on injury nerve site. After 12, 24, and 48 h, only the PLGA conduit groups exposed to 0.05 W/cm(2), 3 min/treatment of ultrasound exhibited decreased LDH release and increased MTT values compared to the sham groups. Based on the results of the in vitro experiment in LDH and MTT testing, the silicone conduits with seeded Schwann cells group was ignored in the in vivo study. The PLGA nerve conduits seeded with Schwann cells (9 x 10(3) cells) were implanted to 15-mm right sciatic nerve defects in rats. Each conduit received 12 ultrasonic treatment sessions over 2 weeks after 1 day of rest. Ultrasound was applied as follows: frequency, 1MHz; intensity, 0.3 W/cm(2) (SATP); treatment, 5 min/day. Implanted graft specimens were harvested for histological analysis at 8 weeks following surgery. PLGA groups (with and without Schwann cells) treated with pulsed ultrasonic stimulation were found to have significantly greater number and area of regenerated axons at the mid-conduit of implanted grafts, as compared to the sham groups. Ultrasonic stimulation on silicone groups was found to induce a mass of fibrous tissues that covered the nerve conduits and retarded axon regeneration.

show abstract

Section: Discussionmentioning

confidence: 99%

Low‐intensity‐ultrasound–accelerated nerve regeneration using cell‐seeded poly(D,L‐lactic acid‐co‐glycolic acid) conduits: An in vivo and in vitro study

et al. 2005

View full text Add to dashboard Cite

show abstract

“…In addition to improving extraction efficiency and mass transfer, the succession of compression and rarefaction phases induced by US creates cavitation bubbles, which asymmetrically collapse onto the surface of cell walls producing high shear stresses, while transient cavitation does the main damage in liver cell membranes through high temperatures and pressures [36] . The high pressure and temperature released during this event dissociate molecular bonds [37] and generate microjets directed towards the cell’s membrane, destroying it and releasing the enzymes from the matrix. Other processes attributed to cavitation inside the cells, such as dispersal tissue, cell destruction, and intensive blending may also contribute to improving mass transfer through the destruction of boundary diffusional layers [38] , [39] , [40] , [41] .…”

Section: Resultsmentioning

confidence: 99%

Ultrasonic-assisted extraction and automated determination of catalase and lipase activities in bovine and poultry livers using a digital movie-based flow-batch analyzer

Siqueira

Almeida

Fernandes

et al. 2021

Ultrasonics Sonochemistry

View full text Add to dashboard Cite

Highlights Ultrasonic-assisted extraction of catalase and lipase from bovine and poultry livers. Successful coupling of a lab-made ultrasonic reactor to a flow-batch analyzer. Lab-made ultrasonic reactor with efficiency 40 times higher than that of mechanical stirring. Accurate catalase and lipase determinations with relative errors lower than 2.1%.

show abstract

Direct gene transfer to plant protoplasts by mild sonication

Joersbo¹,

Brunstedt²

1990

Plant Cell Reports

View full text Add to dashboard Cite

A novel procedure employing mild sonication for transformation of plant protoplasts is described. Transient expression of a chloramphenicol acetyltransferase (CAT) gene in protoplasts of sugar beet (Beta vulgaris L.) and tobacco (Nicotiana tabacum L.) was obtained by a brief exposure of the protoplasts to 20 kHz ultrasound in the presence of plasmid DNA. Maximum levels of CAT activity were achieved by sonication for 500-900 ms at 30-70 W electric power (0.65-1.6 W/cm2 acoustic power). This reduced the viability to 15-20 % and 60 % for sugar beet and tobacco protoplasts, respectively. Up to 12 % (sugar beet) and 81 % (tobacco) of maximum transient expression could be achieved with no significant loss of viability. Protoplasts surviving exposure to ultrasound were found to have a similar long-term viability and to regenerate to micro-calli as untreated protoplasts. Plasmid DNA concentrations of 80-110 μg/ml and sucrose concentrations of 21-28 % in the sonication medium were found to be optimal for transient expression.

show abstract

Biological effects of acoustic cavitation

Cited by 18 publications

References 36 publications

Low‐intensity‐ultrasound–accelerated nerve regeneration using cell‐seeded poly(D,L‐lactic acid‐co‐glycolic acid) conduits: An in vivo and in vitro study

Low‐intensity‐ultrasound–accelerated nerve regeneration using cell‐seeded poly(D,L‐lactic acid‐co‐glycolic acid) conduits: An in vivo and in vitro study

Ultrasonic-assisted extraction and automated determination of catalase and lipase activities in bovine and poultry livers using a digital movie-based flow-batch analyzer

Direct gene transfer to plant protoplasts by mild sonication

Contact Info

Product

Resources

About