Biological Effects of Amelogenin Exon 5 Encoded Peptide from Enamel Matrix Derivative in Human Dental Pulp Cells

Kato, Hirohito; Taguchi, Yoichiro; Tominaga, Kazuhiro; Noguchi, Masahiro; Imai, Kazutaka; Ruan, Yaru; Takahashi, Saitatsu; Kimura, Daisuke; Shida, Muneyasu; Taguchi, Reiko; Maeda, Hiroshi; Tanaka, Akio; Umeda, Makoto

doi:10.20944/preprints201801.0037.v1

Cited by 4 publications

(15 citation statements)

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“…In line with this data, with the use of Multiplex ELISA (using commercially-customized U-PLEX human biomarkers) in our study, we showed that UV-C-killed bacteria significantly induced pro-inflammatory cytokines secretion-IL-6, IL-8, IL-1b and granulocyte macrophage colony-stimulating factor (GM-CSF)-especially by gram-negative F. nucleatum and grampositive P. yurii. Consistent with our results, several studies showed that LPS of periodontal pathogen P. gingivalis and LPS from E. coli can stimulate the secretion of the same pro-inflammatory cytokines IL-6 and IL-8 in periodontal-ligament-derived, gingiva-derived MSCs and SCAP (Wang and Ohura, 2002;Jönsson et al, 2008;Trubiani et al, 2012;Herath et al, 2013;Wang et al, 2013;Kato et al, 2014;Andrukhov et al, 2016;Diomede et al, 2017). This proinflammatory cytokines secretion was also observed for grampositive P. yurii, which exerts the LTA ligand for TLR2, and it was shown by several studies that induction of TLR2 in human periodontal-ligament-derived stem cells also resulted in the upregulation of IL-8, IL-6, and MCP-1 (in our study we did not observe the statistically significant increase of the MCP-1, except the trend towards it) (Blufstein et al, 2018;Behm et al, 2019).…”

Section: Discussionsupporting

confidence: 91%

“…Siqueira, 2019). It was shown that LPS of gram-negative bacteria, LTA of gram-positive, and bacterial lipoproteins can stimulate mesenchymal stem cells inflammatory response, and reduce their osteogenic potential (Tom-Kun Yamagishi et al, 2011;Morsczeck et al, 2012;Kato et al, 2014;Nguyen and Götz, 2016;Yu et al, 2019;Lei et al, 2020;Yin et al, 2020;Casey et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Exploring the impact of oral bacteria remnants on stem cells from the Apical papilla: mineralization potential and inflammatory response

Zymovets,

Rakhimova,

Wadelius

et al. 2023

Front. Cell. Infect. Microbiol.

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IntroductionBacterial persistence is considered one of the main causal factors for regenerative endodontic treatment (RET) failure in immature permanent teeth. This interference is claimed to be caused by the interaction of bacteria that reside in the root canal with the stem cells that are one of the essentials for RET. The aim of the study was to investigate whether prolonged exposure of stem cells from the apical papilla (SCAP) to bacterial remnants of Fusobacterium nucleatum, Actinomyces gerensceriae, Slackia exigua, Enterococcus faecalis, Peptostreptococcaceae yurii, commonly found in infected traumatized root canals, and the probiotic bacteria Lactobacillus gasseri and Limosilactobacillus reuteri, can alter SCAP’s inflammatory response and mineralization potential.MethodsTo assess the effect of bacterial remnants on SCAP, we used UV-C–inactivated bacteria (as cell wall-associated virulence factors) and bacterial DNA. Histochemical staining using Osteoimage Mineralization Assay and Alizarin Red analysis was performed to study SCAP mineralization, while inflammatory and osteo/odontogenic-related responses of SCAPs were assessed with Multiplex ELISA.ResultsWe showed that mineralization promotion was greater with UV C–inactivated bacteria compared to bacterial DNA. Immunofluorescence analysis detected that the early mineralization marker alkaline phosphatase (ALP) was increased by the level of E. coli lipopolysaccharide (LPS) positive control in the case of UV-C–inactivated bacteria; meanwhile, DNA treatment decreased the level of ALP compared to the positive control. SCAP’s secretome assessed with Multiplex ELISA showed the upregulation of pro-inflammatory factors IL-6, IL-8, GM-CSF, IL-1b, neurotrophic factor BDNF, and angiogenic factor VEGF, induced by UV-C–killed bacteria.DiscussionThe results suggest that long term stimulation (for 21 days) of SCAP with UV-C–inactivated bacteria stimulate their mineralization and inflammatory response, while DNA influence has no such effect, which opens up new ideas about the nature of RET failure.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Exploring the impact of oral bacteria remnants on stem cells from the Apical papilla: mineralization potential and inflammatory response

Zymovets,

Rakhimova,

Wadelius

et al. 2023

Front. Cell. Infect. Microbiol.

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“…Additionally, remaining pathogens alter the induced release of TGF-β1 from dentin (Cameron et al, 2019). Bacterial lipopolysaccharides could also remain after root canal disinfection and promote pro-inflammatory cytokines production (Kato et al, 2014). Additionally, induction of an inflammatory periapical plug was demonstrated to hinder cell migration into the root canal and subsequently prevent tissue regeneration (Zaky et al, 2020).…”

Section: Disinfection and Regeneration; A Cause-and-effect Relationsh...mentioning

confidence: 99%

Regenerative Endodontics and Minimally Invasive Dentistry: Intertwining Paths Crossing Over Into Clinical Translation

Elnawam

Abdelmougod

Mobarak

et al. 2022

Front. Bioeng. Biotechnol.

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Regenerative endodontic procedures have been described for over a decade as a paradigm shift in the treatment of immature necrotic permanent teeth, owing to their ability to allow root maturation with subsequent enhancement of the tooth’s fracture resistance in addition to the potential for regeneration of vital intracanal tissues. Concomitantly, minimally invasive endodontics is another rising concept with the main concern of preservation of tooth structure. Stemming from their potential to preserve the original tooth structure, both regenerative and minimally invasive endodontics could be considered as two revolutionary sciences with one common goal. Achieving this goal would entail not only employing the appropriate strategies to recreate the ideal regenerative niche but modifying existing concepts and protocols currently being implemented in regenerative endodontics to address two important challenges affecting the outcome of these procedures; conservation of tooth structure and achieving effective disinfection. Therefore, the search for new biomimetic cell-friendly disinfecting agents and strategies is crucial if such a novel integratory concept is to be foreseen in the future. This could be attainable by advocating a new merged concept of “minimally invasive regenerative endodontic procedures (MIREPs),” through modifying the clinical protocol of REPs by incorporating a minimally invasive access cavity design/preparation and biomimetic disinfection protocol, which could enhance clinical treatment outcomes and in the future; allow for personalized disinfection/regeneration protocols to further optimize the outcomes of MIREPs. In this review, we aim to introduce this new concept, its realization and challenges along with future perspectives for clinical implementation.

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“…Many studies have established the immunobiological importance of the lipopolysaccharide of Gram negative cell wall envelope (127,128). It has the ability to activate the host inflammatory responses and disrupt bone remodeling process (129,130). P. gingivalis LPS binds to the CD14 and TLR-4, activating macrophages, epithelial cells respectively, leading to secretion of pro-infammatory cytokines (131,132).…”

Section: Lipopolysaccharides (Lps)mentioning

confidence: 99%

Tobacco-gene activity profile in Porphyromonas gingivalis, Filifactor alocis and Treponema denticola.

Cherukumalli

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Biological Effects of Amelogenin Exon 5 Encoded Peptide from Enamel Matrix Derivative in Human Dental Pulp Cells

Abstract: Enamel matrix derivative (EMD) is used for periodontal tissue regeneration therapy, and 17can induce mineralization in dental pulp cells (DPCs). We designed a synthetic peptide (SP) derived

Cited by 4 publications

References 0 publications

Exploring the impact of oral bacteria remnants on stem cells from the Apical papilla: mineralization potential and inflammatory response

Exploring the impact of oral bacteria remnants on stem cells from the Apical papilla: mineralization potential and inflammatory response

Regenerative Endodontics and Minimally Invasive Dentistry: Intertwining Paths Crossing Over Into Clinical Translation

Tobacco-gene activity profile in Porphyromonas gingivalis, Filifactor alocis and Treponema denticola.

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