Biological Effects of Helium-Neon Laser Irradiation on Normal and Wounded Human Skin Fibroblasts

Hawkins, Denise; Abrahamse, Heidi

doi:10.1089/pho.2005.23.251

Cited by 115 publications

(79 citation statements)

References 11 publications

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“…The results support findings from Hawkins and Abrahamse [33], which reported that 5 J/cm 2 using 632.8 nm stimulates mitochondrial activity, cell proliferation, and migration to accelerate wound closure [33]. However, wounded cells exposed to 5 J/cm 2 using 632.8 nm in IR show an increase in caspase 3/7, increase in cytotoxicity, and decrease in viability when compared to cells exposed in the dark or in the light indicating an undesirable thermal effect of IR light.…”

Section: Resultssupporting

confidence: 91%

Changes in Cell Viability of Wounded Fibroblasts following Laser Irradiation in Broad‐Spectrum or Infrared Light

Hawkins

Abrahamse

2006

Laser Chemistry

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Objective. This study aimed to establish if broad-spectrum or infrared (IR) light in combination with laser therapy can assist phototherapy to improve the cell function of wounded cells. Background. The effect of laser light may be partly or completely reduced by broad-spectrum light. Methods. Wounded human skin fibroblasts were irradiated with 5 J/cm 2 using a helium-neon laser, a diode laser, or an Nd:YAG laser in the dark, in the light, or in IR. Changes in cell viability were evaluated by cell morphology, ATP cell viability, LDH membrane integrity, and caspase 3/7 as an early marker of apoptosis. Results. Wounded cells exposed to 5 J/cm 2 using 632.8 nm in the dark or 830 nm in the light or 1064 nm in the dark showed an increase in ATP viability, an increase in cytokine expression, and a decrease in LDH cytotoxicity indicating that the metabolic activity of the wounded cells was stimulated. Conclusion. Wounded cells irradiated in IR light showed an undesirable thermal effect that was proportional to the duration of exposure.

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Section: Resultssupporting

confidence: 91%

Changes in Cell Viability of Wounded Fibroblasts following Laser Irradiation in Broad‐Spectrum or Infrared Light

Hawkins

Abrahamse

2006

Laser Chemistry

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“…1 It is broadly applied to reduce inflammation, 2 promote tissue regeneration, 3 relieve pain 4 enhance healing process of wound 5,6 and increase cell proliferation. 7,8 The molecular mechanisms of biological responses after LLL exposure have not been fully demonstrated. It is believed that physiological effects induced by LLL depend on the absorption of energy by some molecular photoacceptors or chromophores in the target cells.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of 660 nm Low-Level Laser and Light Emitted Diode in Proliferative Effects of Fibroblast Cells

et al. 2017

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Introduction: In recent years, low-power lasers have been widely used in medicine. With the introduction of affordable light emitted diode (LED), clinical application of LED light has become more and more popular. However, some researchers believe that due to lack of coherence of the LED light, it can be different in terms of biological effects, in comparison with laser. In this study, the biological effects of low-level laser (LLL) to those of LED light are compared and discussed. Methods: Human skin fibroblast cell line Hu02 was irradiated with LLL and LED light with a wavelength of 660 nm, power output of 35 mW and in continuous mode and the control group was not irradiated. The biological effects were compared through analysis of cell proliferation, production of reactive oxygen species (ROS) within the cell and rate of cell division. Results: Our findings showed that production of ROS within the cell was linearly increased both in the LED and laser light irradiated cells. However, laser light is more incremental in comparison to LED light. The MTT results showed that laser light at low energy density (less than 5 J/cm 2 ) increased the rate of cell proliferation after 24 hours. Although, the rate of cell division was increased in energy density of 1 J/cm 2 compared to the control group, this increase was not statistically significant. Discussion: The findings indicated that the coherence properties of laser light provided more energy for the cells, and in a constant energy density, laser light created more oxidative stresses in comparison with LED light.

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“…For wound healing type studies, these cells are likely to be endothelial cells, 7 fibroblasts, 8 keratinocytes, 9 and possibly some classes of leukocytes. 10 Corneal epithelial repair involves a multifaceted series of events with specific physiologic functions.…”

Section: Discussionmentioning

confidence: 99%

Effect of Photobiomodulation on Wound Healing of the Corneal Epithelium through Rho-GTPase

et al. 2017

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Background and ObjectivesIn recent years, photobiomodulation (PBM) using low-level light has been applied to diverse clinical approaches because of its potential to elevate the cell metabolism or regulate various signaling pathways. This study evaluated the possibility of a short term effect of PBM on the wound healing of corneal epithelial cells. Rapid healing of the corneal epithelium and the return of an intact basement membrane can restore the eye's normal mechanical barriers. The migration, proliferation, attachment, and cytoskeletal rearrangement play a critical role in the wound healing process of the corneal epithelium. Materials and MethodsTo determine which wavelength was most effective on corneal epithelium wound healing, light emitting diode (LED) arrays with wavelengths of 470, 530, 660, 740, and 850 nm were used. The proliferative effect was assessed using a MTT assay, cell cycle assay, and BrdU immunofluorescence (IF) staining, and the motility effect was examined using a wound healing assay after PBM. The cytoskeletal rearrangement effect of PBM was also evaluated by Western blot analysis and IF staining.Results PBM had no effect on cell proliferation; the cell cycle portion and BrdU were not changed after the PBM treatment, whereas cell survival was decreased at 470 nm. On the other hand, PBM at wavelengths greater than 660 nm affected migration. In particular, 740 nm was the most effective. The expression of Rho A and Rho C increased after PBM at wavelengths greater than 660 nm. The levels of cdc42 and mTORC2 expression were similar. ConclusionThis study showed that PBM could increase the corneal epithelial cell migration capacity without cell proliferation in a short time via the activation of a part of Rho-GTPase pathways without the effect of the upstream signals. These findings may be used for the future development of PBM-based therapy for acute ocular surface diseases. Key words

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Biological Effects of Helium-Neon Laser Irradiation on Normal and Wounded Human Skin Fibroblasts

Abstract: A dose of 10 J/cm2 appeared to produce a significant amount of cellular and molecular damage, which could be an important consideration for other therapies, such as photodynamic therapy.

Cited by 115 publications

References 11 publications

Changes in Cell Viability of Wounded Fibroblasts following Laser Irradiation in Broad‐Spectrum or Infrared Light

Changes in Cell Viability of Wounded Fibroblasts following Laser Irradiation in Broad‐Spectrum or Infrared Light

A Comparative Study of 660 nm Low-Level Laser and Light Emitted Diode in Proliferative Effects of Fibroblast Cells

Effect of Photobiomodulation on Wound Healing of the Corneal Epithelium through Rho-GTPase

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