Biological Effects of Low Background Radiation: Prospects for Future Research in the Low-Background Laboratory DULB-4900 of Baksan Neutrino Observatory INR RAS

Zarubin, Mikhail; Kuldoshina, O. A.; Кравченко, Е. В.

doi:10.1134/s1063779621010056

Cited by 5 publications

(8 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our experiment was performed in the low-background laboratory DULB-4900 of the Baksan Neutrino Observatory of the Institute for Nuclear Research of the Russian Academy of Sciences (BNO INR RAS / Neutrino village, North Caucasus, Russia) which has unique shielding properties [ 7 , 19 , 32 ]. It is located in the farthest part (3700 meters from the entrance) of the horizontal tunnel in the Andyrchy mountain at the depth of 1800 meters under the rock mass (4900 m.w.e.)…”

Section: Methodsmentioning

confidence: 99%

“…The study of biological effects of background radiation reduction and determination of the value of very low-dose radiation for living organisms belong to a rapidly evolving field of interdisciplinary research which is focused on fundamental principles of the interaction between ionizing radiation and biological matter, damage-dose relations models, mechanisms of low-dose sensitivity, biological evolution and adaptation. The emphasis of such studies lies on the search for innovations which can be employed in astrobiology, cancer medicine and longevity research [ 3 , 5 – 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…Effects of background ionizing radiation deprivation on organisms have been studied for the last two decades [ 6 , 11 – 17 ] in experiments performed in various locations, for instance, in the low-background chamber at Osaka University (Osaka, Japan) and several industrial underground tunnels, such as DUGL CJEM (Erdaogou Mine, China), BISAL (Boulby, UK) and WIPP (New Mexico, USA) [ 12 , 14 , 16 , 18 ]. But most of biological studies dealt with deep underground laboratories, namely: LNGS (Gran Sasso, Italy), LSM CNRS (Modane, France), SNOLAB (Sudbury, Canada), JINR/BNO INR RAS (Neutrino village, Russia) [ 7 , 11 , 15 , 17 , 19 – 21 ]. They were specially designed for neutrino low-radiation background observatories and are the most efficient places for experimental isolation from cosmic and terrestrial sources of radiation.…”

Section: Introductionmentioning

confidence: 99%

“…To summarize, the explanation of low background radiation effects on living organisms may widen our knowledge about mechanisms of radiosensitivity, hormesis processes, signaling role of ROS and make possible to improve radiation risk model in the range of natural background and low doses of ionizing radiation [ 7 , 16 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

“…Also, we declare our work to be the first initiative of interdisciplinary studies in the BNO (INR RAS) facility which links tasks of biophysics, radiobiology, astrobiology and medicine. The unique DULB-4900 laboratory has a high potential for hosting biological experiments [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

First transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory

et al. 2021

View full text Add to dashboard Cite

Natural background radiation is a permanent multicomponent factor. It has an influence on biological organisms, but effects of its deprivation still remain unclear. The aim of our work was to study for the first time responses of D. melanogaster to conditions of the Deep Underground Low-Background Laboratory DULB-4900 (BNO, INR, RAS, Russia) at the transcriptome level by RNA-seq profiling. Overall 77 transcripts demonstrated differential abundance between flies exposed to low and natural background radiation. Enriched biological process functional categories were established for all genes with differential expression. The results showed down-regulation of primary metabolic processes and up-regulation of both the immune system process and the response to stimuli. The comparative analysis of our data and publicly available transcriptome data on D. melanogaster exposed to low and high doses of ionizing radiation did not reveal common DEGs in them. We hypothesize that the observed changes in gene expression can be explained by the influence of the underground conditions in DULB-4900, in particular, by the lack of stimuli. Thus, our study challenges the validity of the LNT model for the region of background radiation doses below a certain level (~16.4 nGy h-1) and the presence of a dose threshold for D. melanogaster.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

First transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory

et al. 2021

View full text Add to dashboard Cite

show abstract

Cytobacillus pseudoceanisediminis sp. nov., A Novel Facultative Methylotrophic Bacterium with High Heavy Metal Resistance Isolated from the Deep Underground Saline Spring

et al. 2022

View full text Add to dashboard Cite

Protracted Exposure to a Sub-background Radiation Environment Negatively Impacts the Anhydrobiotic Recovery of Desiccated Yeast Sentinels

Lapointe,

Laframboise,

Pirkkanen

et al. 2024

Health Physics

View full text Add to dashboard Cite

Experiments that examine the impacts of subnatural background radiation exposure provide a unique approach to studying the biological effects of low-dose radiation. These experiments often need to be conducted in deep underground laboratories in order to filter surface-level cosmic radiation. This presents some logistical challenges in experimental design and necessitates a model organism with minimal maintenance. As such, desiccated yeast (Saccharomyces cerevisiae) is an ideal model system for these investigations. This study aimed to determine the impact of prolonged sub-background radiation exposure in anhydrobiotic (desiccated) yeast at SNOLAB in Sudbury, Ontario, Canada. Two yeast strains were used: a normal wild type and an isogenic recombinational repair-deficient rad51 knockout strain (rad51Δ). Desiccated yeast samples were stored in the normal background surface control laboratory (68.0 nGy h−1) and in the sub-background environment within SNOLAB (10.1 nGy h−1) for up to 48 wk. Post-rehydration survival, growth rate, and metabolic activity were assessed at multiple time points. Survival in the sub-background environment was significantly reduced by a factor of 1.39 and 2.67 in the wild type and rad51∆ strains, respectively. Post-rehydration metabolic activity measured via alamarBlue reduction remained unchanged in the wild type strain but was 26% lower in the sub-background rad51∆ strain. These results demonstrate that removing natural background radiation negatively impacts the survival and metabolism of desiccated yeast, highlighting the potential importance of natural radiation exposure in maintaining homeostasis of living organisms.

show abstract

Biological Effects of Low Background Radiation: Prospects for Future Research in the Low-Background Laboratory DULB-4900 of Baksan Neutrino Observatory INR RAS

Cited by 5 publications

References 44 publications

First transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory

First transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory

Cytobacillus pseudoceanisediminis sp. nov., A Novel Facultative Methylotrophic Bacterium with High Heavy Metal Resistance Isolated from the Deep Underground Saline Spring

Protracted Exposure to a Sub-background Radiation Environment Negatively Impacts the Anhydrobiotic Recovery of Desiccated Yeast Sentinels

Contact Info

Product

Resources

About