Biological efficacy of perpendicular type-I collagen protruded from TiO2-nanotubes

Chen, Chia-Yu; Kim, David M.; Lee, Cliff; Silva, John Da; Nagai, Shigemi; Nojiri, Toshiki; Nagai, Masazumi

doi:10.1038/s41368-020-00103-3

Cited by 12 publications

(9 citation statements)

References 44 publications

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“…There have been many trials to increase fibroblast attachment to titanium surfaces by using bioactive protein coatings such as fibroblast growth factor-2 [119], laminin [120], fibronectin [121,122], and collagen type-1 [123]. These techniques successfully increase fibroblast attachment, but the use of growth factors or cytokines should be well managed to maintain their activity, while coating techniques suffer from the propensity of detachment at the interface between the base material and coating.…”

Section: Discussionmentioning

confidence: 99%

Ultraviolet Treatment of Titanium to Enhance Adhesion and Retention of Oral Mucosa Connective Tissue and Fibroblasts

Ikeda

Ueno

Saruta

et al. 2021

IJMS

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Peri-implantitis is an unsolved but critical problem with dental implants. It is postulated that creating a seal of gingival soft tissue around the implant neck is key to preventing peri-implantitis. The objective of this study was to determine the effect of UV surface treatment of titanium disks on the adhesion strength and retention time of oral connective tissues as well as on the adherence of mucosal fibroblasts. Titanium disks with a smooth machined surface were prepared and treated with UV light for 15 min. Keratinized mucosal tissue sections (3 × 3 mm) from rat palates were incubated for 24 h on the titanium disks. The adhered tissue sections were then mechanically detached by agitating the culture dishes. The tissue sections remained adherent for significantly longer (15.5 h) on the UV-treated disks than on the untreated control disks (7.5 h). A total of 94% of the tissue sections were adherent for 5 h or longer on the UV-treated disks, whereas only 50% of the sections remained on the control disks for 5 h. The adhesion strength of the tissue sections to the titanium disks, as measured by tensile testing, was six times greater after UV treatment. In the culture studies, mucosal fibroblasts extracted from rat palates were attached to titanium disks by incubating for 24, 48, or 96 h. The number of attached cells was consistently 15–30% greater on the UV-treated disks than on the control disks. The cells were then subjected to mechanical or chemical (trypsinization) detachment. After mechanical detachment, the residual cell rates on the UV-treated surfaces after 24 and 48 h of incubation were 35% and 25% higher, respectively, than those on the control surfaces. The remaining rate after chemical detachment was 74% on the control surface and 88% on the UV-treated surface for the cells cultured for 48 h. These trends were also confirmed in mouse embryonic fibroblasts, with an intense expression of vinculin, a focal adhesion protein, on the UV-treated disks even after detachment. The UV-treated titanium was superhydrophilic, whereas the control titanium was hydrophobic. X-ray photoelectron spectroscopy (XPS) chemical analysis revealed that the amount of carbon at the surface was significantly reduced after UV treatment, while the amount of TiOH molecules was increased. These ex vivo and in vitro results indicate that the UV treatment of titanium increases the adhesion and retention of oral mucosa connective tissue as a result of increased resistance of constituent fibroblasts against exogenous detachment, both mechanically and chemically, as well as UV-induced physicochemical changes of the titanium surface.

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Section: Discussionmentioning

confidence: 99%

Ultraviolet Treatment of Titanium to Enhance Adhesion and Retention of Oral Mucosa Connective Tissue and Fibroblasts

Ikeda

Ueno

Saruta

et al. 2021

IJMS

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“…Hence, it was concluded that electrophoretic fusion of type-I collagen in nanotube titanium can be used to fabricate more robust soft tissue seals. [169]. Electrophoretic deposition has several advantages and disadvantages, which are mentioned in the Table 9.…”

Section: Advantages/disadvantages Referencesmentioning

confidence: 99%

Surface Engineering Strategies to Enhance the In Situ Performance of Medical Devices Including Atomic Scale Engineering

Sultana

Zare

Luo

et al. 2021

IJMS

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Decades of intense scientific research investigations clearly suggest that only a subset of a large number of metals, ceramics, polymers, composites, and nanomaterials are suitable as biomaterials for a growing number of biomedical devices and biomedical uses. However, biomaterials are prone to microbial infection due to Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), hepatitis, tuberculosis, human immunodeficiency virus (HIV), and many more. Hence, a range of surface engineering strategies are devised in order to achieve desired biocompatibility and antimicrobial performance in situ. Surface engineering strategies are a group of techniques that alter or modify the surface properties of the material in order to obtain a product with desired functionalities. There are two categories of surface engineering methods: conventional surface engineering methods (such as coating, bioactive coating, plasma spray coating, hydrothermal, lithography, shot peening, and electrophoretic deposition) and emerging surface engineering methods (laser treatment, robot laser treatment, electrospinning, electrospray, additive manufacturing, and radio frequency magnetron sputtering technique). Atomic-scale engineering, such as chemical vapor deposition, atomic layer etching, plasma immersion ion deposition, and atomic layer deposition, is a subsection of emerging technology that has demonstrated improved control and flexibility at finer length scales than compared to the conventional methods. With the advancements in technologies and the demand for even better control of biomaterial surfaces, research efforts in recent years are aimed at the atomic scale and molecular scale while incorporating functional agents in order to elicit optimal in situ performance. The functional agents include synthetic materials (monolithic ZnO, quaternary ammonium salts, silver nano-clusters, titanium dioxide, and graphene) and natural materials (chitosan, totarol, botanical extracts, and nisin). This review highlights the various strategies of surface engineering of biomaterial including their functional mechanism, applications, and shortcomings. Additionally, this review article emphasizes atomic scale engineering of biomaterials for fabricating antimicrobial biomaterials and explores their challenges.

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“…The surface was created through an electrochemical anodization process using a titanium piece (anode) and a copper piece (cathode). 17,22,23 This way, the negatively charged C-terminus of collagen fibers could be attached perpendicularly to the positively charged TNT surface. These collagen fibers, aligned in an ideal manner, not only demonstrated strong binding resistance against sonification forces, but also inhibited formation of epithelial sheets, suggesting the potential for preventing epithelial downgrowth between the collagen fibers and the titanium surface.…”

Section: Introductionmentioning

confidence: 99%

Microscopic in‐situ analysis of the mucosal healing around implants treated by protease activated receptor 4‐agonist peptide or perpendicularly protruded type I collagen in rats

Song,

Maekawa,

Sasaki

et al. 2023

J Biomed Mater Res

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Enhanced mucosal sealing around titanium implants can reduce complications such as peri‐implantitis. The present study aims to investigate the mucosal healing at the early stage around the protease activated receptor 4‐agonist peptide (PAR4‐AP)‐ or perpendicularly protruded type I collagen (pCol)‐treated titanium implants. A total of 72 implants were placed in 36 rats in the study. Following extractions, two tissue‐level implants among the following three different surfaces, PAR4‐AP‐coated (PAR4 group, n = 24), pCol‐treated (pCol group, n = 24) and non‐treated (control group, n = 24) ones, were placed in the maxillae of each rat based on a split‐mouth design. The specimens retrieved at 8 h (n = 8 per group), 3 days (n = 8 per group), and 2 weeks (n = 8 per group), were immunostained and tissue‐cleared, and the signals of laminin‐5 and collagen fibers were observed under multiphoton microscopy. Statistical analyses were performed using linear mixed model with post hoc tests to compare differences between the groups. While there was no intergroup difference at 8 h, the laminin‐5 at 3 days was more abundant near the PAR4‐group‐surface, and its area was significantly larger in the PAR4 group (0.0204 ± 0.0194 mm2) than the control (0.0019 ± 0.0025 mm2, p = .001) and pCol (0.0023 ± 0.0022 mm2, p < .001) groups. The pCol group showed a significantly larger area of collagen fibers (0.0230 ± 0.0148 mm2) compared to the control (0.0035 ± 0.0051 mm2, p = .002) and PAR4 (0.0031 ± 0.0057 mm2, p < .001) groups at 3 days. At 3 days and 2 weeks, the collagen fiber orientation of the pCol group showed a more perpendicular manner compared to the control and PAR4 groups. The signal of basal lamina and collagen fibers were stronger around the PAR4‐AP‐ and pCol‐treated titanium surfaces, respectively during the early healing stage. This could have implications for improved mucosal sealing around dental implants, potentially reducing complications such as peri‐implantitis.

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Biological efficacy of perpendicular type-I collagen protruded from TiO2-nanotubes

Cited by 12 publications

References 44 publications

Ultraviolet Treatment of Titanium to Enhance Adhesion and Retention of Oral Mucosa Connective Tissue and Fibroblasts

Ultraviolet Treatment of Titanium to Enhance Adhesion and Retention of Oral Mucosa Connective Tissue and Fibroblasts

Surface Engineering Strategies to Enhance the In Situ Performance of Medical Devices Including Atomic Scale Engineering

Microscopic in‐situ analysis of the mucosal healing around implants treated by protease activated receptor 4‐agonist peptide or perpendicularly protruded type I collagen in rats

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