Biological Evaluation of LL‐37 Antimicrobial Peptide‐Loaded Biodegradable Barrier Membranes in Guided Bone Regeneration

Abstract

This study aims to determine the in vitro and in vivo biocompatibility and the differences in LL‐37 releasing patterns of the PLC/Col/LL‐37 sustained releasing membrane and the rapid releasing Col/LL‐37 membrane for use as a barrier membrane in guided bone regeneration. On Days 3 and 7, the PLC/Col/LL‐37 membrane revealed generated osteoclasts, whereas the PLC/Col membrane indicated mature osteoclasts. PLC/Col/LL‐37 was non‐toxic to blood cells and inhibited human albumin adhesion to the surface. A microbial reduction was observed in the Col/LL‐37 membrane, while both PLC/Col/LL‐37 and Col/LL‐37 may have caused early phases of skin sensitization. In animal testing, PLC/Col/LL37 and Col/LL37 exhibited the same levels of inflammation as the control group at all time points, while the different releasing patterns of LL‐37 loaded on PLC/Col/LL‐37 and Col/LL‐37 membranes had no effect on inflammation resolution in rat models.