Biological Fingerprinting Analysis by Liquid Chromatography/Mass Spectrometry for Evaluation of DNA Structural Selectivity of Multiple Compounds in Natural Products

Abstract

A method by combination of centrifugal ultrafiltration (CUF) sampling with liquid chromatography-mass spectrometry (LC-MS) analysis was established to evaluate the DNA structure and sequence selectivity of the multiple compounds in a small molecule library. The developed method was applied to analyze the extracts of natural products Coptis chinensis Franch and Rheum palmatum (L.). From the obtained biological fingerprinting chromatograms, 7 compounds in C. chinensis Franch and 11 in R. palmatum (L.) were screened out as DNA binding agents. Most of these compounds were identified by standards and LC-MS analysis after the sample pretreatment with the DNA immobilized cartridge. DNA structural binding preference of the multiple active compounds in these two extracts was then evaluated simultaneously without purification.

Biological fingerprinting analysis of the traditional Chinese prescription Longdan Xiegan Decoction by on/off-line comprehensive two-dimensional biochromatography

“…Recently we have developed biochromatography methods to probe the interaction of the extracts of TCM with immobilized HSA, ␣ 1 -acid glycoprotein (␣ 1 -AGP) and liposome [7][8][9]. And microdialysis-HPLC was also used for probing the interaction of extracts of TCMs with DNA, ␣ 1 -AGP and HSA in homogeneous phase [10][11][12][13]. BFA, especially using biochromatography, offers a convenient method for the study of interaction between multi-components in the extracts of TCMs and tethered targets.…”

Preparation of “click” binaphthyl stationary phase and its application for separation of anthraquinones from Rheum palmatum L.

“…To evaluate the separation properties of anthraquinones on ''click'' binaphthyl column, a water extract of R. palmatum L. was analyzed. Anthraquinones show characteristic absorption at 220, 280, and 432 nm in UV-VIS spectra [20][21][22]. Thus, the anthraquinones can primarily be observed by comparing the chromatograms at 280 nm with that at 432 nm (Fig.…”

Progress on the Screening and Analysis of Bioactive Compounds in Traditional Chinese Medicines by Biological Fingerprinting Analysis

“…The feasibility of this method for screening and analysis of bioactive compounds in TCMs was verified. In the following years, BFA based on centrifugal ultrafiltration (CUF) sampling combined with HPLC was proposed and compared to sampling by CUF with microdialysis [46]. The recoveries by CUF are much higher than those by microdialysis.…”

“…BFA based on CFU-HPLC was also applied to screening and analysis of the natural products Coptis chinensis Franch and Rheum palmatum (L.). From the obtained biological fingerprinting chromatograms, seven active compounds in C. chinensis Franch and 11 in Rheum palmatum (L.) were screened and identified [46]. On the basis of the microdialysis/CUF-HPLC method, BFA was shown to be effective for screening of bioactive compounds and studying the complex interactions of a single DNA target with multiple components in a TCM.…”