Biological Function of MicroRNA193a-3p in Health and Disease

Grossi, Ilaria; Salvi, Alessandro; Abeni, Edoardo; Marchina, Eleonora; Petro, Giuseppina De

doi:10.1155/2017/5913195

Cited by 47 publications

(45 citation statements)

References 61 publications

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“…Despite advances in the prevention, diagnosis and treatment of cSCC worldwide, the exact molecular basis of cSCC remains unclear ( 5 , 26 , 27 ). miRNAs are considered to be important genetic regulators of various biological processes, including cell proliferation, development, invasion and apoptosis ( 28 – 30 ). A number of previous studies have demonstrated that miRNAs are involved in the pathogenesis of a number of human diseases, particularly different types of cancer ( 12 , 31 , 32 ).…”

Section: Discussionmentioning

confidence: 99%

miR‑186 promotes tumor growth in cutaneous squamous cell carcinoma by inhibiting apoptotic protease activating factor‑1

Tian

Shen

Yan³

et al. 2018

Exp Ther Med

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Cutaneous squamous cell carcinoma (cSCC) accounts for 20% of non-melanoma skin cancer worldwide. MicroRNAs (miRNAs or miRs) are a subtype of non-coding RNA associated with the progression of various types of human cancer. MiR-186 has been demonstrated to act as an oncogene in human tumors. However, the role of miR-186 in cSCC remains unclear. The expression of miR-186 and apoptotic protease activating factor 1 (APAF1) was examined using reverse transcription-quantitative polymerase chain reaction, western blotting and immunofluorescence. The correlation between miR-186 and APAF1 was determined using a dual-luciferase assay. Mimics or inhibitors of miR-186 were transfected into A-431 cells to establish cell lines with overexpressed or knocked-down miR-186, respectively. EdU staining and colony formation assays were performed to detect cell proliferation. Transwell and wound-healing assays were performed to analyze cell invasion and migration, respectively. Hoechst staining and flow cytometry were performed to assess cell apoptosis and cell cycle distribution. MiR-186 expression was significantly increased, while APAF1 expression was significantly decreased in cSCC tissues compared with the controls. An miR-186 binding site was predicted in APAF1 and their expression was negatively correlated in cSCC tissues. Cell proliferation, invasion and migration were significantly enhanced in the miR-186-overexpressed A-431 cells and attenuated in miR-186 knockdown cells compared with the control. APAF1 expression was regulated by miR-186, while APAF1 knockdown significantly promoted cell invasion and inhibited cell apoptosis. In summary, the results of the present study indicate that miR-186 serves as an oncogene in cSCC by inhibiting APAF1.

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Section: Discussionmentioning

confidence: 99%

miR‑186 promotes tumor growth in cutaneous squamous cell carcinoma by inhibiting apoptotic protease activating factor‑1

Tian

Shen

Yan³

et al. 2018

Exp Ther Med

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“…miRNAs have recently become a focus of research. Their main function is post-transcriptional gene regulation in plants and animals (32)(33)(34), and studies have increasingly focused on their function in heart development (35)(36)(37). We identified previously that miR-29c-3p is upregulated in the serum of pregnant women carrying fetuses with CHD (9).…”

Section: Discussionmentioning

confidence: 99%

Akt3 is a target of miR-29c-3p and serves an important function in the pathogenesis of congenital heart disease

Chen

et al. 2018

Int J Mol Med

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Our previous studies identified that the expression of microRNA-29c (miR-29c-3p) was significantly increased in the serum of pregnant women carrying fetuses with congenital heart disease (CHD) compared with in that of normal pregnant women. However, the mechanism by which miR-29c-3p affects development of the embryonic heart remained unclear. The aim of the present study was to investigate the effect and potential molecular mechanism of miR-29c-3p overexpression on P19 cell proliferation, apoptosis and differentiation. miR-29c-3p-overexpression and protein kinase Bγ (Akt3)-knockdown cell lines were constructed using transfection technology. The function of miR-29c-3p and Akt3 in cardiomyocyte development was investigated by determining the proliferation, apoptosis and differentiation of P19 cells, which can differentiate into cardiomyocytes induced by dimethylsulfoxide. Bioinformatic analysis and luciferase assays were performed to explore the association between Akt3 and miR-29c-3p. The results of the present study revealed that miR-29c-3p overexpression and Akt3 knockdown suppressed proliferation, and promoted apoptosis and differentiation in P19 cells. Akt3 was also demonstrated to be a target of miR-29c-3p. Therefore, overexpression of miR-29c-3p may inhibit proliferation, and promote apoptosis and differentiation in P19 cells by inhibiting the expression of Akt3. miR-29c-3p may be a potential therapeutic target for the treatment of CHD.

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“…For instance, miRNAs have been used to target tumor-suppressor mRNAs or oncogene mRNAs [99]. The tumor-suppressor miRNA named miR-193a-3p has been demonstrated to affect proliferation, apoptosis, migration, invasion, and metastasis [100]. In another approach, it was demonstrated that HDAC inhibitors could modulate miR-200 expression, presenting a novel strategy having the potential for breast cancer treatment [101].…”

Section: Cancer and Epigeneticsmentioning

confidence: 99%

Nutrition, Cancer Genetics and Epigenetics

Lundström¹

2019

obm genet

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Epidemiological data and meta-analysis have confirmed that there exists a strong association between nutrition and disease risk. In the context of cancer, it has been demonstrated that unhealthy diets increase the risk of disease. On the other hand, major dietary interventions and lifestyle changes have been demonstrated to provide therapeutic efficacy in cancer patients. Genetic mechanisms have been reported to be associated with cancer development induced by environmental and nutritional factors. Genetics plays an important role in the designing of personalized drugs and diets. Epigenetics has also been demonstrated to be affected by nutrition, leading to increased risk of disease when unhealthy diets are consumed, while healthy nutrition is able to provide both prophylactic and therapeutic efficacy.

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Biological Function of MicroRNA193a-3p in Health and Disease

Cited by 47 publications

References 61 publications

miR‑186 promotes tumor growth in cutaneous squamous cell carcinoma by inhibiting apoptotic protease activating factor‑1

miR‑186 promotes tumor growth in cutaneous squamous cell carcinoma by inhibiting apoptotic protease activating factor‑1

Akt3 is a target of miR-29c-3p and serves an important function in the pathogenesis of congenital heart disease

Nutrition, Cancer Genetics and Epigenetics

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