Biological Hydrogen Production from Biowaste Using Dark Fermentation, Storage and Transportation

Talapko, Domagoj; Talapko, Jasminka; Erić, Ivan; Škrlec, Ivana

doi:10.3390/en16083321

Cited by 13 publications

(6 citation statements)

References 126 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A more or less similar result was also reported by another study [ 24 ]. Many researchers have recorded that, in comparison with other cellulolytic strains, R. albus could ferment many types of carbohydrates, mainly cellulose, glucose, xylose, and mannitol [ 25 ]. Though tests such as indole and catalase were negative ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of strictly anaerobic cellulolytic rumen bacterial species from Sahiwal cattle

Alam,

Hossain,

Sohidullah

et al. 2024

J Adv Vet Anim Res

View full text Add to dashboard Cite

Objective: To isolate, and characterize cellulolytic rumen bacteria from the rumen of Sahiwal cattle utilizing rumen bacterial inoculum to increase the nutritional value of rice bran used as broiler feed. Materials and Methods: The ruminal liquid was kept at an optimal pH of 6.9, and a redox potential of less than -300mV while being incubated anaerobically at 39℃ in a medium containing Rumen Fluid Glucose Cellobiose Agar. By using the Hungate technique, the isolates were detected on the basis of their morphological, physiological, biochemical, and molecular testing. Results: The findings revealed that the isolated Ruminococcus albus, and Ruminococcus flavifaciens were obligate anaerobic, generally Gram-positive, non-motile cocci or rod, single or pair, occasionally short chain, producing yellow pigment when grown on cellulose, and presence of clear zone around the colonies. Both isolates fermented sugars such cellobiose, glucose, and lactose as well as decomposed xylan. The results also showed that the isolates were recognized as Ruminococcus spp., a cellulolytic rumen bacterium, were catalase negative, indole negative, and gelatin liquefaction positive. Conclusion: Isolation and characterization of Ruminococcus spp. may be helpful for Bangladesh in reducing the cost of producing poultry feed, and circumventing restrictions on rice bran use. We can also develop more efficient and long-lasting plans to enhance poultry performance, and feed efficiency as well as increase the nutritional value of rice bran used as broiler feed through understanding how various Ruminococcus spp. function in this process.

show abstract

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of strictly anaerobic cellulolytic rumen bacterial species from Sahiwal cattle

Alam,

Hossain,

Sohidullah

et al. 2024

J Adv Vet Anim Res

View full text Add to dashboard Cite

show abstract

“…Dark fermentation (DF), also known as dark fermentation (DF), stands out as a promising option within the realm of biohydrogen production methods, in which anaerobic microorganisms produce hydrogen utilizing carbohydrate-rich substrate in the absence of light [17]. The key advantage of this green technique includes simple operational requirements, stable hydrogen production, and minimal energy consumption [18]. More importantly, this method can utilize various non-economic biomass as feedstock such as the wastewater and organic fraction of municipal waste, providing enormous economic and environmental benefits over conventional techniques through clean energy supply and reduction in the cost of municipal wastewater and solid waste management [19].…”

Section: Figure 1 Global Hydrogen Production By Methodsmentioning

confidence: 99%

“…The principal organic components within the sludge are carbohydrates, fats, and proteins, which are broken down by hydrogen-producing bacteria such as Clostridium through the DF process [18]. Based on the production stage of the municipal wastewater sludge, it can be classified as the primary sludge (PS) and waste-activated sludge (WAS), generated from the primary and secondary treatment units of the WWTPs [26].…”

Section: Figure 3 Different Processes Of Biohydrogen Productionmentioning

confidence: 99%

Techno-Economic Assessment of Biohydrogen Production from Dark Fermentation of Wastewater Sludge

Alam,

Nayan

2024

Preprint

View full text Add to dashboard Cite

This paper explores a simulation model for biohydrogen production by dark fermentation of wastewater sludge using the Aspen Plus process simulation software. The process includes sludge pretreatment, hydrolysis, fermentation and post-treatment of the products though separation and purification. The outcomes of the model serve as inputs for a techno-economic analysis to assess the economic viability of the process. Additionally, a sensitivity analysis is employed to scrutinize the influence of crucial variables on the overall process. Finally, a greenhouse gas emission potential of the proposed system was obtained from literature, to ensure the environmental benefits from this process. The cost analysis for 23 ton/day sludge processing capacity dark fermentation plant showed a biohydrogen production cost of $11.4/kg of hydrogen produce. The capital cost and the labor cost have been the key contributors to the production cost. The biohydrogen price decreased to $5.9/ kg when the plant was scaled up to 500 ton/day capacity. Examining the greenhouse gas (GHG) emission potential of the process from literature unveiled a substantial reduction in GHG emissions and a carbon credit of $90/ ton CO2-eq can lower the biohydrogen production price to $1.28/kg, rendering it competitive to hydrogen price produced from natural gas-based technology.

show abstract

“…They found that using defined microbial consortia and adjusting factors such as pH, temperature, and substrate concentration could significantly enhance hydrogen yields. Their research demonstrated that optimized conditions could lead to a more robust and scalable process, achieving higher hydrogen production efficiencies [43]. Finally, anaerobic digestion is an established method for methane production, which can be coupled with hydrogen production.…”

Section: Hydorgen Production From Household Biowastementioning

confidence: 99%

Hydrogen Production from Enzymatic Pretreated Organic Waste with Thermotoga neapolitana

Tix,

Moll,

Krafft

et al. 2024

Energies

View full text Add to dashboard Cite

Biomass from various types of organic waste was tested for possible use in hydrogen production. The composition consisted of lignified samples, green waste, and kitchen scraps such as fruit and vegetable peels and leftover food. For this purpose, the enzymatic pretreatment of organic waste with a combination of five different hydrolytic enzymes (cellulase, amylase, glucoamylase, pectinase and xylase) was investigated to determine its ability to produce hydrogen (H2) with the hydrolyzate produced here. In course, the anaerobic rod-shaped bacterium T. neapolitana was used for H2 production. First, the enzymes were investigated using different substrates in preliminary experiments. Subsequently, hydrolyses were carried out using different types of organic waste. In the hydrolysis carried out here for 48 h, an increase in glucose concentration of 481% was measured for waste loads containing starch, corresponding to a glucose concentration at the end of hydrolysis of 7.5 g·L−1. In the subsequent set fermentation in serum bottles, a H2 yield of 1.26 mmol H2 was obtained in the overhead space when Terrific Broth Medium with glucose and yeast extract (TBGY medium) was used. When hydrolyzed organic waste was used, even a H2 yield of 1.37 mmol could be achieved in the overhead space. In addition, a dedicated reactor system for the anaerobic fermentation of T. neapolitana to produce H2 was developed. The bioreactor developed here can ferment anaerobically with a very low loss of produced gas. Here, after 24 h, a hydrogen concentration of 83% could be measured in the overhead space.

show abstract

Biological Hydrogen Production from Biowaste Using Dark Fermentation, Storage and Transportation

Cited by 13 publications

References 126 publications

Isolation and characterization of strictly anaerobic cellulolytic rumen bacterial species from Sahiwal cattle

Isolation and characterization of strictly anaerobic cellulolytic rumen bacterial species from Sahiwal cattle

Techno-Economic Assessment of Biohydrogen Production from Dark Fermentation of Wastewater Sludge

Hydrogen Production from Enzymatic Pretreated Organic Waste with Thermotoga neapolitana

Contact Info

Product

Resources

About