Biological Implications of Dmso Based on a Review of Its Chemical Properties*

Rammler, D. H.; Zaffaroni, A.

doi:10.1111/j.1749-6632.1967.tb34861.x

Cited by 167 publications

(65 citation statements)

References 16 publications

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“…Studies on keratin in hair concluded that DMSO replaces water and dehydrates the protein as it penetrates into the hair shaft (42). The ability of DMSO to substitute or remove water might be expected to alter the molecular configuration of extracellular matrix components and enhance penetration (43). Finally, the fact that verapamil appeared to allow limited penetration of dye into the taste bud (Fig.…”

Section: Discussionmentioning

confidence: 99%

A permeability barrier surrounds taste buds in lingual epithelia

Dando

Pereira

Kurian

et al. 2015

American Journal of Physiology-Cell Physiology

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Epithelial tissues are characterized by specialized cell-cell junctions, typically localized to the apical regions of cells. These junctions are formed by interacting membrane proteins and by cytoskeletal and extracellular matrix components. Within the lingual epithelium, tight junctions join the apical tips of the gustatory sensory cells in taste buds. These junctions constitute a selective barrier that limits penetration of chemosensory stimuli into taste buds (Michlig et al. J Comp Neurol 502: 1003–1011, 2007). We tested the ability of chemical compounds to permeate into sensory end organs in the lingual epithelium. Our findings reveal a robust barrier that surrounds the entire body of taste buds, not limited to the apical tight junctions. This barrier prevents penetration of many, but not all, compounds, whether they are applied topically, injected into the parenchyma of the tongue, or circulating in the blood supply, into taste buds. Enzymatic treatments indicate that this barrier likely includes glycosaminoglycans, as it was disrupted by chondroitinase but, less effectively, by proteases. The barrier surrounding taste buds could also be disrupted by brief treatment of lingual tissue samples with DMSO. Brief exposure of lingual slices to DMSO did not affect the ability of taste buds within the slice to respond to chemical stimulation. The existence of a highly impermeable barrier surrounding taste buds and methods to break through this barrier may be relevant to basic research and to clinical treatments of taste.

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Section: Discussionmentioning

confidence: 99%

A permeability barrier surrounds taste buds in lingual epithelia

Dando

Pereira

Kurian

et al. 2015

American Journal of Physiology-Cell Physiology

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“…either 5.6mM glucose or 20mM glucose and 4 pgg/ml insulin (&I), 40pg/ml insulin (01, or 40 pg/ml insulin + 100 pg/ml soybean trypsin inhibitor I found that addition of DMSO diminished colony outgrowth compared with either insulin or hydrocortisone without DMSO. If insulin normally exerts its growth-stimulating effects in culture through proteolysis of cell membranes, perhaps DMSO, acting as a solvent (Rammler and Zaffaroni, 1967), carries insulin through the plasma membrane before it can act on the membrane.…”

Section: Io-mentioning

confidence: 99%

Insulin and hydrocortisone influences on cultured rat tongue epithelium

Hanks

1979

Archives of Oral Biology

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Summary-Rattongue epithelium was separated from underlying connective tissue by trypsinization, dispersed into single cells and small clusters and plated on cellulose acetate-nitrate filters. collagen gels or plastic substrate in serum-supplemented media. Morphologically, abundance of growth and stratification of epithelium was greatest on floating collagen gels, intermediate on plastic and least on the filters. Cultures on the plastic substrate were used to test the effects of insulin, hydrocortisone, dimethyl sulphoxide, glucose and soybean trypsin inhibitor upon epithelial outgrowth. Cultures were plated at the same density, grown to partial confluency, and analysed with a superimposed point lattice to determine the fraction of total subsurface covered. Cells cultured 1.25-5 x lo5 cells/cm* in serum-supplemented clMEM or McCoy's 5A media produced the best outgrowth. Additional insulin supplementation at 40 pg/ml gave better outgrowth than 400 or 4 pg/ml within the first week. Insulin at 40 pg/ml also gave better outgrowth than a mixed insulin-hydrocortisone supplement at three different levels or than hydrocortisone supplement alone at three different levels. When 0.5 per cent DMSO was added to any of these supplementary formulas, epithelial outgrowth was reduced compared with the same formulae without DMSO. Addition of three times the formula level of glucose also reduced epithelial outgrowth. Addition of soybean trypsin inhibitor to aMEM supplemented with insulin, but not fetal calf serum, caused increased epithelial outgrowth. These findings helped define appropriate conditions for cell attachment, migration, proliferation and differentiation of primary rat tongue epithelial cultures.

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“…Its properties have been studied extensively in the relevant literature since it is used in many applications ranging from medicine to organic synthesis [34]. DMSO is a polar aprotic solvent with a tendency to accept protons higher than water or alcohols, and with lower toxicity than other members of this class, such as dimethylformamide, dimethylacetamide, N-methyl-2-pyrrolidone, and hexamethyl-phosphoramide.…”

Section: Is Dmso Indispensable To Achieve Xl? What Is the Role Of Dmsomentioning

confidence: 99%

Cross‐Linking of Sulfonated Poly(ether ether ketone) by Thermal Treatment: How Does the Reaction Occur?

et al. 2013

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The cross-link reaction between macromolecular chains of sulfonated poly(ether ether ketone) (SPEEK) by thermal treatment above 150 degrees C in presence of dimethylsulfoxide (DMSO) is investigated by various techniques, including elemental analysis, acid-base titration, infrared spectroscopy, water uptake (WU) measurements, and thermogravimetry. The conditions of thermal treatment are analyzed. The cross-linking reaction occurs in at least three more or less activated and deactivated positions with different activation energies, leading to different time dependencies of the cross-link reaction. The role of residual solvent DMSO is studied particularly: the cross-linking depends significantly on the amount of solvent in the membranes. Implications on WU and thermal stability are of particular importance for the development of high performance proton-conducting membranes

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Biological Implications of Dmso Based on a Review of Its Chemical Properties*

Cited by 167 publications

References 16 publications

A permeability barrier surrounds taste buds in lingual epithelia

A permeability barrier surrounds taste buds in lingual epithelia

Insulin and hydrocortisone influences on cultured rat tongue epithelium

Cross‐Linking of Sulfonated Poly(ether ether ketone) by Thermal Treatment: How Does the Reaction Occur?

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