Biological Implications of Polyethylene Glycol and PEGylation: Therapeutic Approaches Based on Biophysical Studies and Protein Structure-Based Drug Design Tools

Raina, Neha; Singh, Amit Kumar; Islam, Asimul

doi:10.1007/978-981-15-8936-2_11

Cited by 4 publications

(2 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PEG is a versatile polymer that is non-toxic and FDA-approved for use in biomedical research applications, including tissue engineering and drug discovery ( Raina et al, 2021 ). Because of the cell-repulsive and non-adhesive properties of PEG, di-amino-PEG is used here as a microsphere surface modifier for facile conjugation and subsequent activation of PEG molecules on the microsphere surface.…”

Section: Resultsmentioning

confidence: 99%

Peptide-Grafted Microspheres for Mesenchymal Stem Cell Sorting and Expansion by Selective Adhesion

Wang

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) have considerable value in regenerative medicine because of their unique properties such as pluripotency, self-renewal ability, and low immunogenicity. Isolation and purification are prerequisites for various biomedical applications of MSCs, and traditional sorting methods are often expensive, complicated, and difficult to apply on a large scale. In addition to purification, the requirement for expansion of cells also limits the further application of MSCs. The purpose of this study was to develop a unique magnetic sorting microsphere to obtain relatively pure and high-yield MSCs in an economical and effective way, that can also be used for the expansion of MSCs. Poly (ethylene glycol) (PEG)-based anti-adhesive treatment of the prepared oleic acid grafted Fe3O4-poly (lactic-co-glycolic acid) magnetic microspheres was performed, and then E7 peptide was covalently grafted onto the treated microspheres. Upon a series of characterization, the magnetic microspheres were of uniform size, and cells were unable to adhere to the PEG-treated surface. E7 grafting significantly improved cell adhesion and proliferation. The results obtained from separate culture of various cell types as well as static or dynamic co-culture showed that selective adhesion of MSCs was observed on the magnetic sorting microspheres. Furthermore, the cells expanded on the microspheres maintained their phenotype and typical differentiation potentials. The magnetic properties of the microspheres enabled sampling, distribution, and transfer of cells without the usage of trypsin digestion. And it facilitated the separation of cells and microspheres for harvesting of MSCs after digestion. These findings have promising prospects for MSC research and clinical applications.

show abstract

Section: Resultsmentioning

confidence: 99%

Peptide-Grafted Microspheres for Mesenchymal Stem Cell Sorting and Expansion by Selective Adhesion

Wang

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Folate decorated PLGA can be synthesized by PEGylation of PLGA polymer based on a process that involves a covalent conjugation of active PEG to a polymeric or therapeutic delivery system [11]. The method of PEGylation can be extended to different nanoformulations, polymers, liposomes, peptides, small molecule drugs, antibodies, and nucleotides [12]. In addition to the biocompatible and biodegradable nature of PEG molecules, they have other advantages of using PEG in nanodrug formulations to prolong the time of nanomaterials in blood circulation without release and tend to accumulate at leaky vasculature sites, carrying the entrapped medicine with them and decreasing polymeric nanoparticle harmfulness [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Investigation of Encapsulated Selenium Nanoparticles with PLGA Polymers Against MCF-7 and HBL Cell Lines

Al-Shreefy

Al-Wasiti

Al-Awady³

2023

Nano Biomedicine and Engineering

View full text Add to dashboard Cite

As cancer-related deaths continue to rise, developments in nanotechnology have emerged as a feasible option for finding successful treatments targeting cancerous cells while avoiding all of the drawbacks of traditional drugs. Selenium nanoparticles (SeNPs) have been reported to exhibit an inhibitory effect on cancerous cells. The aim of the present study was to use the drug delivery systems poly(lactic-coglycolic acid) (PLGA) and poly(lactic-co-glycolic acid-poly(ethylene glycol)-folic acid (PLGA-PEG-FA) to encapsulate SeNPs and investigate their antineoplastic effects against two cell line types (MCF-7 as a positive folate receptor and HBL as a negative folate receptor) by exploiting overexpression features in some types of cancer cells to ensure delivery of drug molecules at high dosages toward targeted cells and circumvent normal cells/tissues. SeNPs were chemically synthesized and characterized with dynamic light scattering (DLS) and transmission electronic microscopy (TEM). The cytotoxicity of both nanomaterials was evaluated against MCF-7 and HBL cells by using the methyl thiazolyl tetrazolium (MTT) assay which showed a high cytotoxic effect against MCF-7 cells with a lesser effect against HBL cells. Additionally, an apoptosis assay was also performed by using acridine orange/ethidium bromide dual staining, and the antioxidant effect was also investigated by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) colorimetric method with high antioxidant potential for both formulations. They showed nonhemolytic activity on human red blood cells. This work could be considered promising for pharmaceutical formulation.

show abstract

Chitosan/Poly (Ethylene Glycol)/ZnO Bionanocomposite for Wound Healing Application

Emam‐Djomeh

Hajikhani

2022

Biodegradable and Environmental Applications of Bionanocomposites

View full text Add to dashboard Cite

Biological Implications of Polyethylene Glycol and PEGylation: Therapeutic Approaches Based on Biophysical Studies and Protein Structure-Based Drug Design Tools

Cited by 4 publications

References 85 publications

Peptide-Grafted Microspheres for Mesenchymal Stem Cell Sorting and Expansion by Selective Adhesion

Peptide-Grafted Microspheres for Mesenchymal Stem Cell Sorting and Expansion by Selective Adhesion

Investigation of Encapsulated Selenium Nanoparticles with PLGA Polymers Against MCF-7 and HBL Cell Lines

Chitosan/Poly (Ethylene Glycol)/ZnO Bionanocomposite for Wound Healing Application

Contact Info

Product

Resources

About