Biological interactions of a calcium silicate based cement (Biodentine™) with Stem Cells from Human Exfoliated Deciduous teeth

Athanasiadou, Eirini; Paschalidou, Maria; Theocharidou, Anna; Kontoudakis, Nikolaos; Arapostathis, Konstantinos; Bakopoulou, Athina

doi:10.1016/j.dental.2018.09.014

Cited by 27 publications

(33 citation statements)

References 43 publications

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“…[33][34][35] In previous work, it was found that both CS and CTS possessed prominent biocompatibility and latent capacity for biomedical applications. [36][37][38] Interestingly, both the structure and chemical composition of the La-MSC/ CTS scaffolds provided a basis for rapid cell adhesion and proliferation. Furthermore, a peak of cell adhesion and proliferation occurred in the Ca3La1/CTS group.…”

Section: Ca3la1/cts Scaffolds Promote Rbmsc Proliferation In Vitromentioning

confidence: 99%

La-Doped mesoporous calcium silicate/chitosan scaffolds for bone tissue engineering

Peng

Liao

et al. 2019

Biomater. Sci.

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Section: Ca3la1/cts Scaffolds Promote Rbmsc Proliferation In Vitromentioning

confidence: 99%

La-Doped mesoporous calcium silicate/chitosan scaffolds for bone tissue engineering

Peng

Liao

et al. 2019

Biomater. Sci.

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“…Ca, Si, Mg, Cu, Zn and P ions, which were detected in a previous study, were selected and their releases from each Biodentine™ extract were evaluated by measuring concentrations in medium with inductively coupled plasma-atomic emission spectrometry (ICP-AES; OPTIMA 8300, PerkinElmer, Boston, MA, USA) [ 35 ]. All the groups were analyzed three times, and the results were averaged.…”

Section: Methodsmentioning

confidence: 99%

“…Thus, biocompatibility and bioactivity for pulp tissue regeneration have been carefully investigated during the development and usage of pulp regenerative materials [ 31 , 32 ]. The biocompatibility and bioactivity of Biodentine™ have been widely reported, with a focus on DPSCs [ 33 , 34 ]; however, only a few studies have focused on SHED [ 35 ]. The pulp tissues of both primary and permanent teeth are embryologically derived from the ectomesenchyme of the neural crest, but distinct differences between them have been reported [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have reported that tricalcium silicate nanoparticle-containing cement (Biodentine™) showed no cytotoxic effect as an extract in contact with DPSCs and SHED [ 35 , 39 , 40 ]. However, previous studies have not taken into account biological effects during setting because this material is usually applied on pulp cells from 3 to 6 min after mixing in cases of vital pulp therapies in clinics.…”

Section: Introductionmentioning

confidence: 99%

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Biological Effects of Tricalcium Silicate Nanoparticle-Containing Cement on Stem Cells from Human Exfoliated Deciduous Teeth

et al. 2020

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Nanomaterials can enhance interactions with stem cells for tissue regeneration. This study aimed to investigate the biological effects of tricalcium silicate nanoparticle-containing cement (Biodentine™) during or after setting on stem cells from human exfoliated deciduous teeth (SHED) to mimic clinically relevant situations in which materials are adapted. Specimens were divided into four groups depending on the start of extraction time (during (3, 6 and 12 min) or after setting (24 h)) and extracted in culture medium for 24 h for further physicochemical and biological analysis. After cell viability in serially diluted extracts was evaluated, odontogenic differentiation on SHED was evaluated by ARS staining using nontoxic conditions. A physicochemical analysis of extracts or specimens indicated different Ca ion content, pH, and surface chemistry among groups, supporting the possibility of different biological functionalities depending on the extraction starting conditions. Compared to the ‘after setting’ group, all ‘during setting’ groups showed cytotoxicity on SHED. The during setting groups induced more odontogenic differentiation at the nontoxic concentrations compared to the control. Thus, under clinically simulated extract conditions at nontoxic concentrations, Biodentine™ seemed to be a promising odontoblast differentiating biomaterial that is helpful for dental tissue regeneration. In addition, to simulate clinical situations when nanoparticle-containing cement is adjusted, biological effects during setting need to be considered.

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“…The water-based calcium silicate materials are the most used bioactive endodontic cements due to their ability to promote biological sealing of damaged dentin and to stimulate cells to proliferate 5 and differentiate 6 resulting in the formation of regenerative tissue in dentin and pulp. Recently, sol-gel derived calcium silicate particles were purposed as an easy method to produce these particles leading to materials with high reactivity and biocompatibility 7 .…”

Section: Introductionmentioning

confidence: 99%

Development of resin-based bioactive endodontic cements with glycerol salicylate and calcium silicate

Pistorello

Balbinot

Leitune

et al. 2020

R. Fac. Odontol. Porto Alegre

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Introduction: The combination of sol-gel derived calcium silicate particles and glycerol salicylate resins may enhance the pyhisico-chemical properties contribute to the understanding of the interaction between these materials. This study aims to evaluate the physical-chemical properties of resin-based bioactive endodontic cements with glycerol salicylate resins and calcium silicate particles. Materials and methods: Calcium silicate was produced by the sol-gel route, while the resin was produced by mixing 60wt% glycerol salicylate, 30wt% methyl salicylate and 10wt% distilled water. Calcium silicate was added in three different concentrations, 50, 40 and 25% by weight. The cement was tested for setting time, flow, radiopacity and pH. Results: The 50:50 group shows the time of 15min8s while the 75:25 shows the time of 256min13s (p

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Biological interactions of a calcium silicate based cement (Biodentine™) with Stem Cells from Human Exfoliated Deciduous teeth

Cited by 27 publications

References 43 publications

La-Doped mesoporous calcium silicate/chitosan scaffolds for bone tissue engineering

La-Doped mesoporous calcium silicate/chitosan scaffolds for bone tissue engineering

Biological Effects of Tricalcium Silicate Nanoparticle-Containing Cement on Stem Cells from Human Exfoliated Deciduous Teeth

Development of resin-based bioactive endodontic cements with glycerol salicylate and calcium silicate

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