Biological investigation of a supersaturated self-nanoemulsifying drug delivery system of Piper cubeba essential oil

Shakeel, Faiyaz; Shazly, Gamal A.; Raish, Mohammad; Ahmad, Ajaz; Kalam, Mohd Abul; Ali, Naushad; Ansari, Mushtaq A.; Elosaily, Gehan M.

doi:10.1039/c5ra22900f

Cited by 22 publications

(39 citation statements)

References 39 publications

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“…The results showed no significant changes in particle size, PDI, and % release of the optimized formulation F4, suggesting that the medicated optimized nanosuspension of MA was sufficiently stable. The stability of nanosuspensions has already been proved previously in the literature [28,[31][32][33][34][35]. Several drugs, such as cyadox, aprepitant, and azoxystrobin, were found to be stable when formulated into nanosuspension formulations [28,31,35].…”

Section: Stability Studymentioning

confidence: 64%

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Investigating the Feasibility of Mefenamic Acid Nanosuspension for Pediatric Delivery: Preparation, Characterization, and Role of Excipients

Perween¹,

Alshehri

Easwari³

et al. 2021

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Molecules with poor aqueous solubility are difficult to formulate using conventional approaches and are associated with many formulation delivery issues. To overcome these obstacles, nanosuspension technology can be one of the promising approaches. Hence, in this study, the feasibility of mefenamic acid (MA) oral nanosuspension was investigated for pediatric delivery by studying the role of excipients and optimizing the techniques. Nanosuspensions of MA were prepared by adopting an antisolvent precipitation method, followed by ultrasonication with varying concentrations of polymers, surfactants, and microfluidics. The prepared nanosuspensions were evaluated for particle size, morphology, and rheological measures. Hydroxypropyl methylcellulose (HPMC) with varying concentrations and different stabilizers including Tween® 80 and sodium dodecyl sulfate (SLS) were used to restrain the particle size growth of the developed nanosuspension. The optimized nanosuspension formula was stable for more than 3 weeks and showed a reduced particle size of 510 nm with a polydispersity index of 0.329. It was observed that the type and ratio of polymer stabilizers were responsive on the particle contour and dimension and stability. We have developed a biologically compatible oral nanoformulation for a first-in-class drug beautifully designed for pediatric delivery that will be progressed toward further in vivo enabling studies. Finally, the nanosuspension could be considered a promising carrier for pediatric delivery of MA through the oral route with enhanced biological impact.

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Section: Stability Studymentioning

confidence: 64%

“…The zeta potentials of the formulations F1-F8 were found to be −17 to −30 mV. The zeta potential value at a magnitude of ±30 mV suggested the maximum stability of nanoformulation [33]. Hence, the formulation F4 was found to be maximally stable compared with the other formulations studied.…”

Section: Nanosuspension Characterizationmentioning

confidence: 88%

Investigating the Feasibility of Mefenamic Acid Nanosuspension for Pediatric Delivery: Preparation, Characterization, and Role of Excipients

Perween¹,

Alshehri

Easwari³

et al. 2021

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“…Gastroprotective, analgesic, anti-inflammatory, and hepatoprotective activity of PO has been investigated in literature (Choi and Hwang 2003;Perazzo et al 2003;Morikawa et al 2004;AlSaid et al 2014AlSaid et al , 2015. More recently, mechanistic anti-inflammatory effects of PO at molecular level have also been reported in animal models (Shakeel et al 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Nanomedicine-based drug delivery systems have great potential in enhancing pharmacological effects and pharmacokinetic profile of drug molecules and plant-based bioactive materials (Jaiswal et al 2015;Shakeel et al 2015;Odei-Addo et al 2017;Thiruvengadam et al 2018;Alam et al 2018). Recently, oral nanoemulsions and "self-nanoemulsifying drug delivery systems (SNEDDS)" have also been evaluated for the enhancement of pharmacological effects of several therapeutic agents (Jain et al 2015;Jaiswal et al 2015;Shakeel et al 2015;Sharma et al 2015;Cui et al 2019;Eleftheriadis et al 2019). SNEDDS are preconcentrates of nanoemulsions in which the drug is encapsulated in oil phase in the presence of surfactant/cosurfactant which could form very fine nanosized droplets/nanoemulsions upon mild agitation with an aqueous media (Villar et al 2012;Jain et al 2015;Shakeel et al 2015;Sharma et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Wound healing evaluation of self-nanoemulsifying drug delivery system containing Piper cubeba essential oil

et al. 2019

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The objective of this work was to characterize and evaluate wound healing potential of Piper cubeba oil (PO) via selfnanoemulsifying drug delivery system (SNEDDS) in comparison with standard gentamycin. PO loaded SNEDDS was prepared by low energy emulsification technique and characterized for thermodynamic stability, self-emulsification power and various physico-chemical parameters. An optimal formula of PO SNEDDS was subjected to wound healing evaluation, collagen determination and histomorphological examination in female Wistar rats as compared with pure PO and standard antibiotic/gentamycin. An optimal formula of PO SNEDDS showed significant wound healing effects in Wistar female rats in comparison with pure PO. However, wound healing effects of optimized SNEDDS were comparable with standard gentamycin. An optimized formulation also indicated significant enhancement in collagen content (0.82 mg/g) in comparison with pure PO (0.53 mg/g) and negative control (0.33 mg/g). While, the collagen content of SNEDDS (0.82 mg/g) treated rats were comparable with standard gentamycin treated animals (0.98 mg/g). Histopathological examinations of optimized SNEDDS treated animals showed no signs of inflammatory cells which indicated that prepared SNEDDS was safe and nontoxic to rats. The results obtained in this work showed the potential application of SNEDDS in enhancement of the wound healing activity of PO upon oral administration.

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“…Hence, the objective of this research work was to develop suitable nanoemulsion formulations of EEO and to investigate its wound healing effects in rat model as compared with pure EEO and standard antibiotic gentamycin after oral administration. Macroemulsions were not prepared in this work because nanoemulsions have lower droplet size in comparison with macroemulsions and these systems are more suitable for enhancing absorption and therapeutic activity of essential oils 11,12,27 . Various nanoemulsion formulations of EEO were prepared using aqueous phase titration method via construction of pseudoternary phase diagrams.…”

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confidence: 99%

Wound Healing Study of Eucalyptus Essential Oil Containing Nanoemulsion in Rat Model

et al. 2018

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The objective of this investigation was to develop nanoemulsion formulations of Eucalyptus essential oil (EEO) and to evaluate its wound healing effects in comparison with standard gentamycin in rat model. Various nanoemulsionns of EEO were prepared using aqueous phase titration method and the zones of nanoemulsion were identified by the construction of phase diagrams. EEO nanoemulsions were investigated in terms of physical stability, self-nanoemulsification efficiency and physicochemical characterization. Optimized nanoemulsion of EEO was selected for wound healing study, collagen estimation and histopathological evaluation in rats in comparison with pure EEO and standard gentamycin. Optimized nanoemulsion presented significant would healing activity in rats as compared with pure EEO upon oral administration. The wound healing activity of optimized nanoemulsion was comparable with standard gentamycin. Optimized EEO nanoemulsion also presented significant enhancement in collagen content as compared with pure EEO and negative control. However, the collagen contents of optimized nanoemulsion treated animals were comparable with standard gentamycin-treated animals. Histopathological studies of optimized nanoemulsion treated rats showed no signs of inflammatory cells which suggested the safety and non-toxicity of EEO nanoemulsion. This study suggested the potential of nanoemulsion in enhancing the wound healing activity of EEO upon oral administration.

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Biological investigation of a supersaturated self-nanoemulsifying drug delivery system of Piper cubeba essential oil

Abstract: In this work, the mechanism of Piper cubeba essential oil anti-inflammatory activity alone and as a supersaturated self-nanoemulsifying drug delivery system (S-SNEDDS) was evaluated.

Cited by 22 publications

References 39 publications

Investigating the Feasibility of Mefenamic Acid Nanosuspension for Pediatric Delivery: Preparation, Characterization, and Role of Excipients

Investigating the Feasibility of Mefenamic Acid Nanosuspension for Pediatric Delivery: Preparation, Characterization, and Role of Excipients

Wound healing evaluation of self-nanoemulsifying drug delivery system containing Piper cubeba essential oil

Wound Healing Study of Eucalyptus Essential Oil Containing Nanoemulsion in Rat Model

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