Biological Isolation and Characterization of Catharanthus roseus (L.) G. Don Methanolic Leaf Extracts and their Assessment of Antimicrobial, Cytotoxic and Apoptotic Activities

Rajashekara, S.; Reena, Dondapati; Mainavi, Mullahalli VenkataramaReddy; Sand, Locheruvapalli Srinivasa; Baro, Utpal

doi:10.21203/rs.3.rs-1831603/v1

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“…The methanolic extracts of C. roseus leaves have incredible contributions to the field of medicinal applications. (Rajashekara et al, 2022). C. roseus flower extracts have a significant antifungal property and are used as antibacterial agents to combat different infections (Neglo et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and expression analysis of TCP family genes in Catharanthus roseus

et al. 2023

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IntroductionThe anti-tumor vindoline and catharanthine alkaloids are naturally existed in Catharanthus roseus (C. roseus), an ornamental plant in many tropical countries. Plant-specific TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factors play important roles in various plant developmental processes. However, the roles of C. roseus TCPs (CrTCPs) in terpenoid indole alkaloid (TIA) biosynthesis are largely unknown.MethodsHere, a total of 15 CrTCP genes were identified in the newly updated C. roseus genome and were grouped into three major classes (P-type, C-type and CYC/TB1).ResultsGene structure and protein motif analyses showed that CrTCPs have diverse intron-exon patterns and protein motif distributions. A number of stress responsive cis-elements were identified in promoter regions of CrTCPs. Expression analysis showed that three CrTCP genes (CrTCP2, CrTCP4, and CrTCP7) were expressed specifically in leaves and four CrTCP genes (CrTCP13, CrTCP8, CrTCP6, and CrTCP10) were expressed specifically in flowers. HPLC analysis showed that the contents of three classic TIAs, vindoline, catharanthine and ajmalicine, were significantly increased by ultraviolet-B (UV-B) and methyl jasmonate (MeJA) in leaves. By analyzing the expression patterns under UV-B radiation and MeJA application with qRT-PCR, a number of CrTCP and TIA biosynthesis-related genes were identified to be responsive to UV-B and MeJA treatments. Interestingly, two TCP binding elements (GGNCCCAC and GTGGNCCC) were identified in several TIA biosynthesis-related genes, suggesting that they were potential target genes of CrTCPs. DiscussionThese results suggest that CrTCPs are involved in the regulation of the biosynthesis of TIAs, and provide a basis for further functional identification of CrTCPs.

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Section: Discussionmentioning

confidence: 99%