Biological markers for metal toxicity

Kakkar, Poonam; Jaffery, Farhat N.

doi:10.1016/j.etap.2004.09.003

Cited by 176 publications

(85 citation statements)

References 131 publications

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“…It has been assumed that fifty percent of human male infertility of unknown cause may be due to environmental and occupational exposures (Evenson and Wixon, 2005). Therefore, male reproductive dysfunction is one of the major effects of lead toxicity (Kakkar and Jaffery, 2005). Compared to other organs, It has been proved that testis is one of main target organs for lead intoxication (Benoff et al, 2000) particularly in rodents (Perera et al, 2002), which are under investigation in the present work.…”

Section: Discussionmentioning

confidence: 99%

Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats

Nasr¹,

Elmadawy²,

Almadaly³

et al. 2017

AJVS

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Key words:Lead toxicity, apoptosis, male rat, testis, garlic Lead is a highly prevalent heavy metal pollutant in human and animals which has various toxicological effects on the different organs including reproductive system on particular aspect the male testis. Lead (Pb) exposure enhances the generation of Reactive Oxygen Species (ROS) which negatively affect testis structure and functions. The present study aimed to investigate the effect of garlic supplement on daily basis and its prophylactic possibility against lead acetate induced testicular dysfunction. Thirty two adult male rats were divided to four groups, eight animals per each. All animals were maintained under standard laboratory conditions with free access to suitable diet and water supply. The 1 st group was supplied with water and diet and kept without further treatment as a negative control. The 2 nd group was treated by lead acetate( 50mg/L) daily in drinking water as a positive control. The 3 rd group was administered commercial garlic powder preparation only (200 mg/kg b.w.) by stomach tube. The 4 th group was co-treated with lead acetate in drinking water plus garlic in same doses by stomach tube. Then, all animals were scarified after 42 days for clean excision of epididymis and testes. Semen was collected and analyzed for count, motility and viability. Testicular tissues sections were prepared, stained and examined. Oxidative stress markers, SOD and MDA were determined in testicular tissue homogenate. Immunostaining was carried out on sections of testicular tissue for detection of proapoptotic markers caspase-3 and Bax expression. Finally, the level of expression of Bcl-2 and p53 genes was measured in testicular tissue samples as well. The data summary obtained from co treated group signified the protective effect of garlic against potential lead acetate induced undesired testicular apoptosis.

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Section: Discussionmentioning

confidence: 99%

Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats

Nasr¹,

Elmadawy²,

Almadaly³

et al. 2017

AJVS

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“…Exposure of the local population may be both environmental 35 and occupational through air, food or drinking water (Kakkar and Jaffery, 2005).…”

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confidence: 99%

Population exposure to trace elements in the Kilembe copper mine area, Western Uganda: A pilot study

Mwesigye

Young

Bailey

et al. 2016

Science of The Total Environment

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The mining and processing of copper in Kilembe, Western Uganda, from 1956 to 1982 left over 15 Mt of 1 tailings containing cupriferous and cobaltiferous pyrite dumped within a mountain river valley. This pilot study 2 was conducted to assess the nature and extent of risk to local populations from metal contamination arising from 3 those mining activities. We determined trace element concentrations in mine tailings, soils, locally cultivated 4 foods, house dust, drinking water and human biomarkers (toenails) using ICP-MS analysis of acid digested trace metals and is also time and dose-dependent (Alissa et. al, 2011). Metal toxicity may 49 result in serious health consequences, causing low productivity with declining economic 50 development and increased health expenditure (Jarup, 2003). Some common health 51 implications of excessive trace element intake include kidney disease, damage to the nervous 52 system, diminished intellectual capacity, diseases of the heart, gastrointestinal system and 53 skeleton, cancer and death (Jarup, 2003). Unfortunately, the extent of chronic metal 54 poisoning is often difficult to assess in developing countries due to resource limitations. whenever the river bursts its banks. There has been substantial mobilisation of sulphate and 64 metallic elements into surrounding river basins and soils (Owor et al., 2007). In addition, 65 potentially contaminated underground mine water discharges into the River Nyamwamba 66 which is a major source of domestic water for local people. Previous studies in the greater 67Rwenzori region have all found elevated concentrations of trace elements including Cu, Co, 68Ni and Zn in the soils, lake water and sediments (Edroma, 1974; Muwanga et al., 1997; 69 Ssenku et al., 2014). However no previous study has explicitly investigated trace elements in

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“…For this reason, wild animals have been used to evaluate exposures to inorganic contaminants, and data collected from these animals are considered very useful for human and environmental health risk assessment. Also, it is widely accepted that this type of assessment cannot be based solely on chemical analyses, and that it should include the measurement of biological effects resulting from pollutants (Walker 1998;Kakkar 2005).…”

Section: Introductionmentioning

confidence: 99%

Apoptosis, metallothionein, and bioavailable metals in domestic mice (Mus musculus L.) from a human-inhabited volcanic area

et al. 2007

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The influence of extreme environments of volcanic origin over vertebrates and the cellular responses that these may give are almost unknown. The main objectives were to evaluate the exposure of mice to metals in the interior of houses of a small village settled inside a volcanic crater (Furnas, Azores), and the levels of apoptosis and metallothionein in the organs (lung, liver, and kidney) of those animals. Adult mice (Mus musculus) were captured in two areas, one with volcanic activity and the other without it over the last three centuries. In the excised organs, analysis of metals (Al, Cd, Pb, Zn), TUNEL assay for apoptosis, and immunohistochemistry for metallothionein were undertook. Mice from the area with volcanic activity presented higher levels of apoptosis and metallothionein than those from the area without volcanic activity. Such results were in agreement with the differences in metal burdens of the three organs, and interestingly these concentrations were similar to or higher than others found in heavily polluted areas outside the Azores. Thus, there may be a high risk of harmful effects for organisms, including humans, inhabiting areas with volcanism, where hazardous gases and metals in the air are very common during the entire day or even all year round.

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Biological markers for metal toxicity

Cited by 176 publications

References 131 publications

Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats

Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats

Population exposure to trace elements in the Kilembe copper mine area, Western Uganda: A pilot study

Apoptosis, metallothionein, and bioavailable metals in domestic mice (Mus musculus L.) from a human-inhabited volcanic area

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