Biological Markers of External and Internal Exposure in Shelter Construction Workers: A 13-Year Experience

Bazyka, Dimitry; Ilienko, I.; Sushko, Viktor; Loganovsky, K.; Lyashenko, L O; Golyarnik, N A; Lyaskivska, O; Nechaev, S.; Shvayko, Lyudmila I.; Bazyka, Kostiantyn; Pilinska, M A; Bezdrobna, L.K.

doi:10.1093/rpd/ncy128

Cited by 3 publications

(2 citation statements)

References 11 publications

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“…Changes in the transcription activity of genes of immune surveillance have been earlier registered in other groups of exposed individuals. For example, excessive expression of immune surveillance and apoptosis genes was observed in persons with cumulative doses of 0.1-113.35 mGy due to trans-uranium radionuclides five years after the onset of exposure (Bazyka et al, 2018). Findings of the study of the gene expression in the Chernobyl Nuclear Plant clean-up workers also demonstrate modulation of activity of more than 100 genes, including genes of cytokines and immune response, in persons with exposure doses > 400 mGy 11-12 years after the onset of radiation exposure (Albanese et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Single nucleotide polymorphism and expression of genes for immune competent cell proliferation and differentiation in radiation-exposed individuals

Блинова

et al. 2020

Vestn. VOGiS

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It is known that ionizing radiation influences the expression of the genes that play a key role in the mechanisms of maintaining the stability of cellular homeostasis. As a rule, changes in the transcriptome of an exposed cell occur within the first 24 hours following radiation exposure. And it predetermines early response in the case of genome damage. Later on modulations in gene transcription activity are also possible and could result in a carcinogenic effect. However, in order to find the role of exogenous factors (ionizing radiation), it is also necessary to take into account the contribution of endogenous factors that are able to modify gene transcription activity. This is especially important for long after the onset of radiation exposure. Single nucleotide polymorphisms located in regulatory regions of the genes may belong to this group of factors. The objective of the current study was to analyze the influence of ionizing radiation on the transcription activity of the STAT3, GATA3, NFkB1, PADI4 genes, which regulate proliferation and differentiation of immune competent human cells; and to assess the potential influence of single nucleotide polymorphisms located in regulatory regions of the genes on the amount of mRNA. The study involved people who had been chronically exposed due to releases of radioactive waste into the Techa River. It was observed that 60 years after the onset of radiation exposure changes in the transcription activity of the NFkB1 and PADI4 genes were registered in people with cumulative doses to RBM within the range 78–3510 mGy. In people who had been chronically exposed, the effect of allelic variations in rs1053023, rs4143094, rs28362491, rs874881 on the level of mRNAs of the STAT3, GATA3, PADI4, NFkB1 genes has not been established.

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Section: Discussionmentioning

confidence: 99%

Single nucleotide polymorphism and expression of genes for immune competent cell proliferation and differentiation in radiation-exposed individuals

Блинова

et al. 2020

Vestn. VOGiS

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“…As radiation accidents are known to be rare events, availability of human biological samples is even rarer. In this context, Bazyka and coworkers reported on promising GE dose-response relationships after irradiation in Chernobyl clean-up workers (Ilienko et al 2018;Ilienko et al 2020), and staff of the "Shelter" and exclusion zone (Bazyka et al 2018;Bazyka et al 2020).…”

Section: Useful Study Designmentioning

confidence: 99%

Gene expression for biodosimetry and effect prediction purposes: promises, pitfalls and future directions – key session ConRad 2021

Ostheim

Amundson

Badie

et al. 2021

International Journal of Radiation Biology

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In a nuclear or radiological event, an early diagnostic or prognostic tool is needed to distinguish unexposed from low-and highly-exposed individuals with the latter requiring early and intensive medical care. Radiation-induced gene expression (GE) changes observed within hours and days after irradiation have shown potential to serve as biomarkers for either dose reconstruction (retrospective dosimetry) or the prediction of consecutively occurring acute or chronic health effects. The advantage of GE markers lies in their capability for early (1-3 day after irradiation), high-throughput, and point-of-care diagnosis required for the prediction of the acute radiation syndrome (ARS). ConclusionsAs a key session of the ConRad conference in 2021, experts from different institutions were invited to provide state-of-the-art information on a range of topics including: (1) Biodosimetry: What are the current efforts to enhance the applicability of this method to perform retrospective biodosimetry? (2) Effect Prediction: Can we apply radiation-induced GE changes for prediction of acute health effects as an approach, complementary to and integrating retrospective dose estimation? (3) High-throughput and Point-of-Care Diagnostics: What are the current developments to make the GE approach applicable as a high-throughput as well as a point-of-care diagnostic platform? (4) Low Level Radiation: What is the lowest dose range where GE can be used for biodosimetry purposes? (5) Methodological Considerations: Different aspects of radiation-induced GE related to more detailed analysis of exons, transcripts and next-generation sequencing (NGS) were reported.

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Brain and Eye as Potential Targets for Ionizing Radiation Impact. Part v – Organic and Functional Changes Correlation on the Example of Certain Diseases

Fedirko,

Babenko,

Kuts

et al. 2023

Probl Radiac Med Radiobiol

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The question about correlation between organic and functional changes in persons, exposed to radiation is still insufficiently studied. Dynamics of morbidity for different forms and classes of non-tumour diseases periodisation, proposed by epidemiologists, suggests the identification of three main periods: «early» (the first 6 post-Chornobyl accident years); «distant» (12–21 years) and «late» (22–30 years). However, the correspondence this periodisation to the results of epidemiological data, without taking into account the clinical features of the diseases, may contribute to the impression, that in the first period after a radiation disaster functional disorders (or autonomic regulation disorders, etc.) prevail in all cases. Meanwhile, the data from ophthalmological and neurological studies, which we aim to demonstrate in this paper, rather indicate the presence of a significant morphological basis for the development of functional disorders in early period after a radiation disaster. The objective of this work is analyse modern experimental, epidemiological and clinical data on the correlation between organic and functional changes, characteristic of radiation cerebro-ophthalmological effects – radiation cataracts, age-related macular degeneration, cerebral small vessel disease, and neurocognitive deficits. Materials and methods. The criteria for inclusion in the analytical review were peer-reviewed publications in PubMed/MEDLINE, Scopus, Web of Science, and manually selected papers; the results of our own research were also used. An additional analysis of the results of examinations conducted in 1991–2004 was performed, which included a total of 11 123 persons irradiated as the result of catastrophy at the Chornobyl NPP. Results. In the first period of radiation cataract development, which is a specific consequence of radiation exposure, morphological changes (lens opacities) are observed, which lead to a decrease in visual function only later. Analysing the correlation between organic and functional changes in the development of diseases, for which ionising radiation exposure is a significant risk factor, we observe a similar picture. For example, CSVD associated with arterial hypertension may be a manifestation of accelerated aging associated with exposure to ionising radiation. Similarly, the initial signs of age-related macular degeneration in radiation-exposed individuals are usually manifested by changes in the morphology of the retina, choroid, and pigment epithelium in the macular area, while functional disorders in the form of decreased central vision and metamorphopsia, etc., occur later. Key words: Chornobyl disaster, ionising radiation, age-related macular degeneration, cataract, morphological changes, small vessel disease, neurocognitive deficit.

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Biological Markers of External and Internal Exposure in Shelter Construction Workers: A 13-Year Experience

Abstract: This study confirms the presence of radiation-induced changes in gene regulation of cell proliferation, telomere function and apoptosis in radiation workers exposed to external and internal exposure at doses above professional limits and increase of compensatory changes.

Cited by 3 publications

References 11 publications

Single nucleotide polymorphism and expression of genes for immune competent cell proliferation and differentiation in radiation-exposed individuals

Single nucleotide polymorphism and expression of genes for immune competent cell proliferation and differentiation in radiation-exposed individuals

Gene expression for biodosimetry and effect prediction purposes: promises, pitfalls and future directions – key session ConRad 2021

Brain and Eye as Potential Targets for Ionizing Radiation Impact. Part v – Organic and Functional Changes Correlation on the Example of Certain Diseases

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