Biological molecule-impregnated polyester: an in vivo angiogenesis study

Fournier, Nancy; Doillon, Charles J.

doi:10.1016/0142-9612(96)87645-9

Cited by 39 publications

(17 citation statements)

References 21 publications

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“…Ensuring the bioactivity of growth factors and other biomolecules delivered by scaffolds is one of the major hurdles in tissue engineering 44–47. This challenge arises primarily due to the incompatibility between proteins and organic solvents used in fabricating scaffolds.…”

Section: Discussionmentioning

confidence: 99%

Novel Polymeric Scaffolds Using Protein Microbubbles as Porogen and Growth Factor Carriers

Nair

Thevenot

Dey

et al. 2010

Tissue Engineering Part C: Methods

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Polymeric tissue engineering scaffolds prepared by conventional techniques like salt leaching and phase separation are greatly limited by their poor biomolecule-delivery abilities. Conventional methods of incorporation of various growth factors, proteins, and/or peptides on or in scaffold materials via different crosslinking and conjugation techniques are often tedious and may affect scaffold's physical, chemical, and mechanical properties. To overcome such deficiencies, a novel two-step porous scaffold fabrication procedure has been created in which bovine serum albumin microbubbles (henceforth MB) were used as porogen and growth factor carriers. Polymer solution mixed with MB was phase separated and then lyophilized to create porous scaffold. MB scaffold triggered substantially lesser inflammatory responses than salt-leached and conventional phase-separated scaffolds in vivo. Most importantly, the same technique was used to produce insulin-like growth factor-1 (IGF-1)–eluting porous scaffolds, simply by incorporating IGF-1–loaded MB (MB-IGF-1) with polymer solution before phase separation. In vitro such MB-IGF-1 scaffolds were able to promote cell growth to a much greater extent than scaffold soaked in IGF-1, confirming the bioactivity of the released IGF-1. Further, such MB-IGF-1 scaffolds elicited IGF-1–specific collagen production in the surrounding tissue in vivo. This novel growth factor–eluting scaffold fabrication procedure can be used to deliver a range of single or combination of bioactive biomolecules to substantially promote cell growth and function in degradable scaffold.

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Section: Discussionmentioning

confidence: 99%

Novel Polymeric Scaffolds Using Protein Microbubbles as Porogen and Growth Factor Carriers

Nair

Thevenot

Dey

et al. 2010

Tissue Engineering Part C: Methods

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show abstract

“…As discussed above, different strategies have been pursued to promote blood perfusion in cardiac grafts, such as co-implantation of endothelial cells and/or addition of angiogenic factors [113][114][115][116][117][118]. Although these approaches can lead to the successful formation of vessels within implanted grafts, the drawback is that the time required for new vessels to form and start carrying blood [6,[119][120][121][122] is higher than the rate at which the death of A C C E P T E D M A N U S C R I P T…”

Section: Future Directions and Summarymentioning

confidence: 99%

Vascularization strategies of engineered tissues and their application in cardiac regeneration

Sun

Altalhi

Nunes

2016

Advanced Drug Delivery Reviews

125

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“…Chang et al [27] published data about new vessel formation around hydroxyapatite implants with scanning electron microscopy and reported complete vascularization of the implants after 3 months. Reports are also available concerning gas plasma treatment of different kinds of implants and vascular prostheses [28,29] . These reports show that the surface properties can influence the attachment of cells on biomaterials by changing the characteristics of the surface.…”

Section: Discussionmentioning

confidence: 99%

Improved Neovascularization of PEGT/PBT Copolymer Matrices in Response to Surface Modification by Biomimetic Coating

Ring¹,

Steinstraesser

Muhr³

et al. 2007

Eur Surg Res

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PEGT/PBT (polyethylene glycol terephthalate/polybutylene terephthalate) copolymer matrices with three different surface coatings [calcium-phosphate (Ca-P), collagen, and gas plasma] were placed into dorsal skinfold chambers of 24 balb/c mice. Untreated PEGT/PBT matrices served as the controls. The basal surfaces of the implants directly contacted the striated skin muscle. Neovascularization of the implants was analyzed by intravital fluorescence microscopy. Microcirculatory observations were performed in the surrounding skin muscle, at the border zone of the implant, and in the center of the implant. The functional vessel density (FVD; mm/mm²), as the length of perfused microvessels per observation area, was measured by computer-assisted analysis. The FVD served as the parameter of neovascularization. At the end of the protocol, histological observation of hematoxylin/eosin-standard-stained sections was performed by light microscopy. The FVD in the center of the implant on day 8 was only observed in gas-plasma-coated (8.8 ± 10.2 mm/mm²) and Ca-P-coated implants (0.8 ± 2.0 mm/mm²). None of the other groups showed perfused microvessels in the center of the implant on day 8 (p < 0.05). The FVD values in the center of the gas-plasma-coated and the Ca-P-coated implants were 20.7 ± 8.2 and 19.2 ± 15.5 mm/mm² as compared with 7.1 ± 17.4 and 7.7 ± 5.9 mm/mm² for collagen-coated and untreated implants on day 16. The histological examination confirmed the profound microvascular ingrowth into the matrix pores of the gas-plasma-treated and the Ca-P-coated copolymer matrices in the center of the implants. The study showed that the ingrowth of microvessels into PEGT/PBT matrices can be accelerated by Ca-P coating and gas plasma treatment in the dorsal skinfold chamber in mice.

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Biological molecule-impregnated polyester: an in vivo angiogenesis study

Cited by 39 publications

References 21 publications

Novel Polymeric Scaffolds Using Protein Microbubbles as Porogen and Growth Factor Carriers

Novel Polymeric Scaffolds Using Protein Microbubbles as Porogen and Growth Factor Carriers

Vascularization strategies of engineered tissues and their application in cardiac regeneration

Improved Neovascularization of PEGT/PBT Copolymer Matrices in Response to Surface Modification by Biomimetic Coating

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