Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers

Petković, Vladana; Keta, Otilija; Vidosavljevic, M.; Incerti, S.; Ristić-Fira, Aleksandra; Petrović, Ivana

doi:10.1080/09553002.2019.1549753

Cited by 14 publications

(18 citation statements)

References 32 publications

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“…They show that proton and carbon-ion irradiations reduce survival of MCF-7 and HTB177 cells, with carbon ions being more efficient. As found in previous studies, DMSO possesses good free radical scavenging features, which makes it an effective agent for reducing indirect effects of low and high LET irradiation (Hirayama et al, 2009(Hirayama et al, , 2014Petković et al, 2019;Zwicker et al, 2019). Moreover, pretreatment with DMSO decreases the effects of irradiation itself in both cell lines.…”

Section: Survival Of Mcf-7 and Htb177cells After Irradiations With Prmentioning

confidence: 60%

“…Although several types of cultured cells were used in preclinical radiobiological trials, yet there is a lack of data on the RBE variations in human tumors (Kagawa et al, 2002;Willers et al, 2018). According to our previous results, MCF-7 and HTB177 cells have similar response to γ-radiation with the SF2 values of 0.67 and 0.64, respectively, which positions them among the radioresistant human malignant cells (Fertil & Malaise, 1985;Matthews et al, 2011;Petković et al, 2019). In this work, we aimed to investigate effects of proton and carbon ions on these two cancer cell lines and to set up experimental conditions which would probably enable realistic comparison of direct effects of these ions and their numerical simulations using the Geant4-DNA toolkit (Bernal et al, 2015;Incerti et al, 2014).…”

Section: Discussionmentioning

confidence: 92%

“…For the analysis of changes in cell cycling as well as of protein expression, the irradiation dose of 3 Gy is chosen, considered as an intermediate dose level for both cell lines (Petković et al, 2019). After incubation for 24 and 48 h, irradiated cells were trypsinized, centrifuged, and washed with PBS.…”

Section: Cell Cycle Analysismentioning

confidence: 99%

“…Western blot analysis was performed as described previously (Petković et al, 2019). Briefly, cell lysates were collected at 0.5 h and 24 h following irradiations with 3 Gy, resolved by SDS-PAGE electrophoresis and then transferred to Immobilon-P PVDF membranes (Millipore, Bedford, USA).…”

Section: Analysis Of Protein Expressionmentioning

confidence: 99%

“…To examine the relative biological effectiveness of protons and carbon ions in the present study, comparison with the reference gamma irradiation data is made. Based on the fact that D10 values regarding γ-rays for MCF-7 and HTB177 cell lines are 5.82 Gy and 5.66 Gy, respectively (Petković et al, 2019), one can evaluate RBE D10 as given in Tables 1 and 2. For proton irradiated MCF-7 cells RBE D10 is 1.47, while for those irradiated with carbon ions it is 2.13.…”

Section: Survival Of Mcf-7 and Htb177cells After Irradiations With Prmentioning

confidence: 99%

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DNA damage assessment of human breast and lung carcinoma cells irradiated with protons and carbon ions

Ristić-Fira

Keta

Petković

et al. 2020

Journal of Radiation Research and Applied Sciences

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Differences in biological response of breast and lung cancer cells to 62 MeV/u therapeutic protons and carbon ions are investigated on MCF-7 and HTB177 cells. Hydroxyl radical scavenger, dimethyl sulfoxide (DMSO) is applied to reduce indirect effects of irradiation, while serum deprivation provided uniformity of cell population. Survival, immunocytochemical, and cell-cycle analysis, changes in protein expression involved in repair and apoptosis are followed. Radiobiological parameters, Bax/Bcl-2 ratio, and increased subG1 fraction show that carbon ions are more efficient in cellular killing than protons. No significant difference in the number of γH2AX foci are found between protons and carbon ions. According to SF2 values, MCF-7 cells are more radioresistant to both ion species compared to HTB177 cells. Cell-cycle analysis and expression of relevant protein markers further confirm somewhat higher radiosensitivity of HTB177 cells compared to MCF-7. DMSO leads to the rise of cell survival and decreases the number of γH2AX foci. Even though there are no significant changes in the number of γH2AX foci after used irradiation types, lesions induced by carbon ions are probably more complex than those produced by protons. DMSO minimizes indirect DNA damage to achieve experimental conditions needed for comparisons of obtained results with numerical simulations.

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Section: Survival Of Mcf-7 and Htb177cells After Irradiations With Prmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 92%

Section: Cell Cycle Analysismentioning

confidence: 99%

Section: Analysis Of Protein Expressionmentioning

confidence: 99%

Section: Survival Of Mcf-7 and Htb177cells After Irradiations With Prmentioning

confidence: 99%

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DNA damage assessment of human breast and lung carcinoma cells irradiated with protons and carbon ions

Ristić-Fira

Keta

Petković

et al. 2020

Journal of Radiation Research and Applied Sciences

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Simulation of DNA damage using Geant4‐DNA: an overview of the “molecularDNA” example application

Chatzipapas

Tran

Dordevic³

et al. 2023

Precision Radiation Oncology

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Purpose:The scientific community shows great interest in the study of DNA damage induction, DNA damage repair, and the biological effects on cells and cellular systems after exposure to ionizing radiation. Several in silico methods have been proposed so far to study these mechanisms using Monte Carlo simulations. This study outlines a Geant4-DNA example application, named "molecularDNA", publicly released in the 11.1 version of Geant4 (December 2022).Methods: It was developed for novice Geant4 users and requires only a basic understanding of scripting languages to get started. The example includes two different DNA-scale geometries of biological targets, namely "cylinders" and "human cell". This public version is based on a previous prototype and includes new features, such as: the adoption of a new approach for the modeling of the chemical stage, the use of the standard DNA damage format to describe radiation-induced DNA damage, and upgraded computational tools to estimate DNA damage response.Results: Simulation data in terms of single-strand break and double-strand break yields were produced using each of the available geometries. The results were compared with the literature, to validate the example, producing less than 5% difference in all cases.Conclusion: "molecularDNA" is a prototype tool that can be applied in a wide variety of radiobiology studies, providing the scientific community with an open-access base for DNA damage quantification calculations. New DNA and cell geometries for the "molecularDNA" example will be included in future versions of Geant4-DNA.

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Radiation sensitivity of biological systems, its modification by chemical modifiers and its quantitative evaluation

Múčka,

Čuba

2024

J Radioanal Nucl Chem

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Large number of sensitizers and protectors of cells, tissues, organs and organisms are being discussed in this review. Attention is paid to metals, nanoparticles or organic compounds as modifiers. They can modify the radiation sensitivity by 20–60%. In most cases, sensitization is caused by an increase in DNA damage or a decrease in the repair of radiation damage. The protection can be caused in many cases by repair of damaged DNA molecules, proteins or enzymes, or by reducing the ROS concentration. The most current research tasks in this area are formulated. A new quantitative and reproducible method of evaluating modifiers is discussed.

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Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers

Cited by 14 publications

References 32 publications

DNA damage assessment of human breast and lung carcinoma cells irradiated with protons and carbon ions

DNA damage assessment of human breast and lung carcinoma cells irradiated with protons and carbon ions

Simulation of DNA damage using Geant4‐DNA: an overview of the “molecularDNA” example application

Radiation sensitivity of biological systems, its modification by chemical modifiers and its quantitative evaluation

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