Biological, pathological and physical characterization of a possible variant of a murine sarcoma virus (Moloney)

Chirigos, Michael A.; Scott, David; Turner, W.; Perk, K.

doi:10.1002/ijc.2910030207

Cited by 50 publications

(20 citation statements)

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“…To this purpose we used a variant of the Moloney murine sarcoma virus, the myeloproliferative sarcoma virus (MPSV), which was isolated by Chirigos et al (8) and characterized by Pragnell et al (29). This virus was chosen because of its wide cellular host range and the availability of a temperature-sensitive mutant for transformation (23,25,26).…”

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confidence: 99%

A mos oncogene-containing retrovirus, myeloproliferative sarcoma virus, transforms rat thyroid epithelial cells and irreversibly blocks their differentiation pattern

Portella¹,

Fiore

Berlingieri³

et al. 1985

J Virol

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Differentiated, cloned rat thyroid epithelial cells (424 cells) were infected with a wild-type and a temperature-sensitive strain of the myeloproliferative variant of the Moloney murine sarcoma virus. The thyroid cells were productively infected and transformed by both virus strains and displayed some of the typical properties of malignant cells, such as morphological changes, growth in soft agar, and in vivo tumorigenicity. The transformation of differentiated cells has provided, in the last few years, a model for studying the correlation between transformed and differentiated phenotypes (1). Most of these studies have been performed on cell systems of mesenchymal origin transformed by acute retroviruses. Very few systems are available of epithelial cells having well-defined biochemical markers of differentiation. A very suitable system for this purpose is represented by the rat thyroid epithelial differentiated cell line (FRT-L cell line). This line consists of epithelial cells which continue to express in vitro the most typical markers of thyroid differentiation (thyroglobulin synthesis and secretion, iodide uptake, and growth dependence on six growth factors including thyrotropin, the physiological thyroid stimulator; 2, 3).We have recently demonstrated that the Kirsten murine sarcoma virus (KiMSV) can transform in vitro undifferentiated as well as differentiated rat thyroid cells of a definite epithelial origin (14, 15). The transformation of these cells is evidenced by morphological criteria, growth in semisolid medium, and tumorigenicity after injection into syngeneic animals (14, 15). Transformation of the differentiated cells is associated with the loss of the markerE of epithelial thyroid differentiation (15). The infection of FRT-L cells with a KiMSV mutant temperature sensitive for transformation is followed by the appearance of the transformation markers and the loss of differentiation at the permissive temperature (33°C); the switch from the permissive to the nonpermissive temperature (39°C) does not cause the reappearance of the differentiated functions, while the transformation markers disappear (10, 37).To verify whether the ability to transform rat thyroid epithelial cells is peculiar to KiMSV or is common to other * Corresponding author. acute retroviruses, we infected thyroid cells with a different murine acute retrovirus. To this purpose we used a variant of the Moloney murine sarcoma virus, the myeloproliferative sarcoma virus (MPSV), which was isolated by Chirigos et al. The results presented here indicate clearly that the MPSV has the abilities to transform epithelial thyroid cells of rat origin in vitro and to inhibit the expression of the differentiated phenotype after transformation. The loss of the differentiated markers is irreversible, since FRT-L cells transformed with an MPSV mutant temperature sensitive for transformation irreversibly lose the differentiated phenotype at both permissive and nonpermissive temperatures. Since the infection of FRT-L cells with this mutant at the non...

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confidence: 99%

A mos oncogene-containing retrovirus, myeloproliferative sarcoma virus, transforms rat thyroid epithelial cells and irreversibly blocks their differentiation pattern

Portella¹,

Fiore

Berlingieri³

et al. 1985

J Virol

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“…It is nevertheless difficult to compare the terminal phase of the 78A1 virusinduced syndrome to that of the MPSV-induced disease, since mice infected with 78A1 are afflicted relatively rapidly by a paralysis which kills them within a few days. Such hind leg paralysis has previously been observed in MPSV-infected mice (Chirigos et al, 1968; M. C. Le Bousse-Kerdiles, unpublished results). In our study, we show that this paralysis is due to the cloned 78A1 virus and not to the associated helper .…”

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confidence: 67%

Study of the 78A1 Isolate of Moloney Murine Sarcoma Virus. II. Haematopoietic Tropism and Tumourigenicity

Bousse-Kerdilès

Duménil

Smadja-Joffe

et al. 1985

Journal of General Virology

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“…MPSV is a member of the Moloney murine sarcoma virus (Mo-MuSV) family containing the mos oncogene within a defective Mo-MuLV genome (21,22). It is unique to other described Mo-MuSV variants in that it not only induces sarcomas but also a myeloproliferative disorder, including spleen focus formation in adult mice (22).…”

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Retroviral mutants efficiently expressed in embryonal carcinoma cells.

Franz¹,

Hilberg²,

Seliger³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

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The myeloproliferative sarcoma virus (MPSV) is a unique member of the Moloney murine sarcoma virus family. Due to mutations in the U3 region of its long terminal repeat, MPSV has an expanded host range that includes cells of the hematopoietic compartment. Using a MPSV recombinant containing the gene for neomycin-resistance (NeoR-MPSV), we demonstrate that the host range of MPSV also includes undifferentiated F9 embryonal carcinoma cells. Transfer of G418-resistance with NeORMPSV to F9 cells is almost as efficient as G418-resistance transfer to fibroblasts, in contrast to G418-resistance transfer to PCC4 embryonal carcinoma cells, which is at least 3 orders of magnitude lower.To isolate NeoRMPSV mutants that are efficiently expressed in PCC4 cells, G418-resistant PCC4 cell lines were induced to differentiate, and the provirus was rescued by superinfection with murine leukemia'virus. Viral isolates (PCMV-5 and -6; PCMV = PCC4 cell-passaged Neo".MPSV) were obtained and assayed for expression in embryonal carcinoma cells. The efficiency of NeoR transfer was equally as high in both F9 and PCC4 as in fibroblasts. mos oncogene expression was unaltered as judged by transformation capability. No gross alteration in the coding region and in the long terminal repeat was detectable by restriction enzyme analysis. NeoR-MPSV and its mutants PCMV-5 and -6 can thus be utilized as vectors for the efficient transduction of genes into embryonic cells.

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Biological, pathological and physical characterization of a possible variant of a murine sarcoma virus (Moloney)

Abstract: The biological characteristics of a sarcoma virus and the pathogenesis of the disease induced by it are described. The present agent was derived from a transplantable rhabdomyosarcoma originally induced by the muriiie sarcoma virus ( Moloney) .

Cited by 50 publications

References 4 publications

A mos oncogene-containing retrovirus, myeloproliferative sarcoma virus, transforms rat thyroid epithelial cells and irreversibly blocks their differentiation pattern

A mos oncogene-containing retrovirus, myeloproliferative sarcoma virus, transforms rat thyroid epithelial cells and irreversibly blocks their differentiation pattern

Study of the 78A1 Isolate of Moloney Murine Sarcoma Virus. II. Haematopoietic Tropism and Tumourigenicity

Retroviral mutants efficiently expressed in embryonal carcinoma cells.

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