Biological potency and binding affinity of monoiodoinsulin with iodine in tyrosine A14 or tyrosine A19

Diabetes due to secretion of a structurally abnormal insulin (insulin Wakayama). Clinical and functional characteristics of [LeuA3] insulin.

“…insulin molecule (28)(29)(30)(31)(32). It is therefore likely that this neutral for neutral amino acid substitution produced subtle conformational changes.…”

A method for the iodination of insulin and its binding to dissociated mouse mammary cells.

“…Monoiodoinsulin labelled by the chloramine-T method is reported to have high bioactivity and high binding activity to insulin receptors (Freychet et al, 1971). Al4 tyrosine-monoiodoinsulin, which is the major product (75%) of lactow•roxidase iodination (Hamlin and Arquilla, 1974), is known to maintain its entire bioactivity whereas A19-or B-iodinated insulin has lower bioactivity (Gliemann et al, 1979). Linde and Hansen (1974) separated iodoinsulins into 5 bands (uniodinated insulin, A19-or Bmonoiodoinsulin, Al 4-monoiodoinsulin, diiodoinsulin and triiodoinsulin) on acrylamide gel electrophoresis using long columns.…”

“…Availability of iodoinsulin and mammary cells with intact biological activity seemed necessary for further investigation into insulin-receptor interaction in the mammary gland. Several methods have been developed to iodinate insulin (Freychet et al, 1971;Hamlin and Arquilla, 1974;Gliemann et al, 1979). We have been iodinating prolactin under mild conditions using lactoperoxidase (Sakai et al, 1975 Binding activity of iodinated insulin to mammary cell Two-to three-month-old pregnant and lactating KA mice were used.…”

Receptor-mediated degradation by rat adipocytes: Comparison of A-14 with A-19 125I-labelled insulin.

“…Japon. June 1984 shows only one half of ability of A-14 insulin (Gliemann et al, 1979;Linde et al, 1981), and that this decreased receptorbinding ability of A-19 insulin is not due to an increased dissociation rate but due to a decreased association rate in the study using cultured human lymphocytes (Watanabe et al, 1984). Since there has been no detailed report on their degradation in adipocytes in which receptor-mediated degradation is the major process for insulin degradation, we investigated these aspects of the labelled insulins using isolated rat adipocytes.…”