Biological potential and chemical composition of bioactive compounds from endophytic fungi associated with thai mangrove plants

Sopalun, Kathawut; Laosripaiboon, Wanpen; Wachirachaikarn, Anyalak; Iamtham, Siriluck

doi:10.1016/j.sajb.2021.04.031

Cited by 35 publications

(23 citation statements)

References 33 publications

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“…When 1% cetyl trimethyl ammonium bromide (CTAB) was added to the plate containing 1% pectin, a transparent halo appeared around the colony, which indicated pectinase activity. After the fungi were cultured on PDA plates containing 1% skim milk powder, a transparent hydrolytic halo appeared around the colony, which indicated protease activity ( Sopalun and Iamtham, 2020 ; Liu et al, 2020c ; Sopalun et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Regulating Root Fungal Community Using Mortierella alpina for Fusarium oxysporum Resistance in Panax ginseng

Wang

Suo

et al. 2022

Front. Microbiol.

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Plant-associated microbes play important roles in plant health and disease. Mortierella is often found in the plant rhizosphere, and its possible functions are not well known, especially in medical plants. Mortierella alpina isolated from ginseng soil was used to investigate its effects on plant disease. The promoting properties and interactions with rhizospheric microorganisms were investigated in a medium. Further, a pot experiment was conducted to explore its effects on ginseng root rot disease. Physicochemical properties, high-throughput sequencing, network co-occurrence, distance-based redundancy analysis (db-RDA), and correlation analysis were used to evaluate their effects on the root rot pathogen. The results showed that Mortierella alpina YW25 had a high indoleacetic acid production capacity, and the maximum yield was 141.37 mg/L at 4 days. The growth of M. alpina YW25 was inhibited by some probiotics (Bacillus, Streptomyces, Brevibacterium, Trichoderma, etc.) and potential pathogens (Cladosporium, Aspergillus, etc.), but it did not show sensitivity to the soil-borne pathogen Fusarium oxysporum. Pot experiments showed that M. alpina could significantly alleviate the diseases caused by F. oxysporum, and increased the available nitrogen and phosphorus content in rhizosphere soil. In addition, it enhanced the activities of soil sucrase and acid phosphatase. High-throughput results showed that the inoculation of M. alpina with F. oxysporum changed the microbial community structure of ginseng, stimulated the plant to recruit more plant growth-promoting bacteria, and constructed a more stable microbial network of ginseng root. In this study, we found and proved the potential of M. alpina as a biocontrol agent against F. oxysporum, providing a new idea for controlling soil-borne diseases of ginseng by regulating rhizosphere microorganisms.

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Section: Methodsmentioning

confidence: 99%

Regulating Root Fungal Community Using Mortierella alpina for Fusarium oxysporum Resistance in Panax ginseng

Wang

Suo

et al. 2022

Front. Microbiol.

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“…As mentioned earlier, Aspergillus species are an important resource of novel active phytochemicals with potential therapeutic values [48]. The biggest advantages of endophytic fungi are their fast renewal, relatively easy cultivation and economic and environmentally safety [49]. Numerous bioactive substances with a wide range of activities were found in endophytic fungi over the last 20 years [50].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic Potential of Bioactive Compounds From Aspergillus Flavus, an Endophytic Fungus Isolated From Cynodon dactylon: Experimental and Computational Approach

Kalimuthu¹,

Pavadai²,

Panneerselvam³

et al. 2022

Preprint

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Endophytic fungi are a diverse group of microorganisms that colonize the inter- or intracellular spaces of plants for mutual benefits. The interactions with a host plant and other microbiomes are multidimensional and play a crucial role in the production of secondary metabolites. We screened bioactive compounds present in the extracts of Aspergillus flavus, an endophytic fungus isolated from the roots of the medicinal grass Cynodon dactylon, for its anticancer potential. Ethyl acetate extract from isolated A. flavus showed significant cytostatic effects (IC50: 16.25 μg mL−1) against breast cancer cells (MCF-7). Morphology of cells and DAPI stained nuclei along with the results of flow cytometry annexin V/PI assay suggested apoptosis to be the main process leading to cells’ death. While investigating the mechanism that triggers apoptosis, we found that the extract of A. flavus increased ROS generation and caused loss of mitochondrial membrane potential of MCF-7 cells. To identify the metabolites that might be responsible for the anticancer effect, the extract was examined using gas chromatography-mass spectrometry (GC-MS). Interestingly, nine phytochemicals were found to have potential inhibitory effects of anti-apoptotic protein (Bcl-2) in the breast cancer cells. In the in silico molecular docking and molecular dynamics simulation studies revealed that two compounds: 2,4,7-Trinitrofluorenone and 3alpha, 5alpha-Cyclo-ergosta-7,9(11),22t-triene-6beta-ol exhibited significant binding affinities (-9.20, and -9.50 Kcal mol-1, respectively) against Bcl-2 along with binding stability and intermolecular interactions of its ligand-Bcl-2 complexes. Overall, the study found that the endophytic A. flavus from C. dactylon contains plant-like bioactive compounds that have a promising effect in breast cancer.

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“…64.4% of isolated endophytic fungi from three tropical plants with medicinal properties exhibited cellulase activity 22 . 30% of endophytic fungi isolated from Thai mangrove plants exhibited cellulase activity 24 . Cellulases produced by endophytic fungi not only help them in gaining nutrients (by hydrolyzing cellulosic substrates to glucose) but also assist the host in defending against pathogen attacks 25 .…”

Section: Screening For Extracellular Enzymesmentioning

confidence: 99%

Isolation and Characterization of L-Asparaginase Producing Endophytic Fungi from Medicinal Plants of Rutaceae Family

Udayan¹,

Gnanadoss²

2023

Biosci., Biotech. Res. Asia

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Endophytic fungi synthesize a diverse set of enzymes and biomolecules with wide array of bioactivities. Thirty-five distinct fungal endophytes have been isolated from medicinal plants of Rutaceae and screened for their ability to produce various industrially important enzymes. Primary enzyme screening revealed that 65% of isolates produced L-Asparaginase, 62% of isolates produced cellulase, 60% of isolates produced amylase, 48% of isolates produced both lipase and pectinase, 45% of isolates produced laccase and 42% of isolates produced protease. Among the different enzymes screened, L-Asparaginase producing isolates were dominant and subjected to quantification using the nesslerization method. The endophytic isolates LCJ315, LCJ324, LCJ326 and LCJ335 were capable of producing high L-Asparaginase activity and their enzyme activity ranged between 7.58 U/ml to 8.84 U/ml. The four isolates were further subjected to molecular identification using 18S rRNA sequencing. The results were then subjected to BLAST analysis. The phylogenetic tree was created by MEGA software. The isolated endophytic fungal strains were identified as LCJ315 (Aspergillus sp.), LCJ326 (Colletotrichum sp.), LCJ324 and LCJ335 (Fusarium sp.). The fungal source of L-Asparaginase is preferred over other microbial sources of L-Asparaginase due to reduced side effects. Given the fact that L-Asparaginase is a crucial anticancer enzyme that is used as the initial therapy for acute lymphoblastic leukemia. Hence, the present study reveals that these endophytic fungal strains isolated from Rutaceae members can be used as an alternate source for L-Asparaginase production.

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Biological potential and chemical composition of bioactive compounds from endophytic fungi associated with thai mangrove plants

Cited by 35 publications

References 33 publications

Regulating Root Fungal Community Using Mortierella alpina for Fusarium oxysporum Resistance in Panax ginseng

Regulating Root Fungal Community Using Mortierella alpina for Fusarium oxysporum Resistance in Panax ginseng

Cytotoxic Potential of Bioactive Compounds From Aspergillus Flavus, an Endophytic Fungus Isolated From Cynodon dactylon: Experimental and Computational Approach

Isolation and Characterization of L-Asparaginase Producing Endophytic Fungi from Medicinal Plants of Rutaceae Family

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