Biological Properties of Bacteria Clostridium Perfringens Isolated in the Middle Volga Region From Calves With Anaerobic Enterotoxemia

Spiridonov, A.G.; Makhmutov, A.F.; Spiridonov, Gennady N.; Khuramshina, M.T.; Dupleva, L.Sh.; Nasertdinov, D.D.

doi:10.33632/1998-698x.2021-1-41-46

Cited by 2 publications

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“…have been studied for IBK: Moraxella bovis (Irby and Angelos 2018), Moraxella bovoculi (Karthik et al 2022), and Moraxella ovis. M. ovis is rare and not often found in cattle eye cultures, previous information about it referred to M. bovoculi (Spiridonov et al 2017). M. bovoculi, a recently described species, has been isolated and characterized in association with infectious cattle keratoconjunctivitis (Loy et al 2014(Loy et al , 2021Hille et al 2020;Karthik et al 2022).…”

Section: Introductionmentioning

confidence: 99%

“…IBK, according to many researchers, occupies a leading place in the infectious pathology of cattle (Abdullah et al 2015;Wynn et al 2022;Zhanabayev et al 2022). It causes great economic damage to animal husbandry (Kuibagarov et al 2018) due to a decrease in milk yield by up to 50%, body weight gain by 31-37%, death of animals, as well as the high costs of carrying out therapeutic and preventive measures (Spiridonov et al 2017;Wynn et al 2022).…”

Section: Introductionmentioning

confidence: 99%

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Metagenomic Analysis and Identification of Epizootic Strains of the Causative Agent of Infectious Bovine Keratoconjunctivitis in Kazakhstan

2023

Int J Vet Sci

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This research aimed to identify the causative agent of infectious bovine keratoconjunctivitis (IBK) Moraxella strains and differentiate them using 16S rRNA sequencing and PCR. The 16S ribosomal RNA (16S rRNA) gene sequencing method was used to determine the microbiome. This is due to the high commonness of this gene in the genomes of all prokaryotes, which allows selecting universal primers to strengthen areas of interest and reduce the cost and time of research. PCR-RT was used for the identification of Moraxella strains. In this study, we identified the causative agents Moraxella bovis and Moraxella bovoculi based on the metagenomics analysis of infected cattle in the Republic of Kazakhstan. We visualized the obtained data using Krona's diagram and EDGE bioinformatics software. We differentiated M. bovis and M. bovoculi from other cultures of Moraxella spp. using PCR-RT. For the first time in Kazakhstan, strains that cause IBK were identified using metagenomic analysis. This raises the diagnosis of IBK, a widespread infectious cattle disease, to a genetic level. Our research will serve as the foundation for the further scientific study of the accompanying microorganisms, followed by recommendations for herd welfare and the prevention of economic losses associated with the death and therapeutic manipulations of sick animals.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Metagenomic Analysis and Identification of Epizootic Strains of the Causative Agent of Infectious Bovine Keratoconjunctivitis in Kazakhstan

2023

Int J Vet Sci

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“…Идентификация микроорганизмов -это процесс, в ходе которого проводится определение видовой принадлежности микроба [12]. Для этого врачами-бактериологами или научными сотрудниками используются различные тесты, основанные на изучении наиболее важных фенотипических признаков исследуемого возбудителя [1,3,4,7,10,11,14]. Установление таксономической принадлежности выделенного микроба проводится согласно Международному определителю бактерий Берджи по совокупности основных признаков, присущих данному виду бактерий [13].…”

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Application of Amoxicillin in the pearl necklace test in the identification of the Anthraxic cause

Rodionov

2023

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Identification of the causative agent of anthrax is an integral part of the diagnosis of the disease caused by it, which is registered annually on the territory of our country among animals and people. According to the guidelines for the diagnosis of the causative agent of anthrax, one of the identification tests is to study the sensitivity of the pathogen to penicillin with the registration of the phenomenon of the pearl necklace. Currently, penicillin salts are a hard-to-reach antibacterial drug. Based on this, we used amoxicillin, a penicillin-type antibiotic with a similar mechanism of action, which was the purpose of our study. A nutrient medium with an antibiotic was prepared by analogy with a medium with the addition of penicillin, according to MUC 4.2.2413-08. Amoxicillin in the form of amoxicillin trihydrate produced by Hemofarm (Serbia) was used as an antibacterial drug. To work, amoxicillin, by analogy with penicillin, was diluted 1,000,000 times with sterile Hottinger broth and introduced into a prepared nutrient medium. The loop of the grown daily culture of the strain K-STI-79 B. anthracis was seeded in 3 ml of the prepared medium and incubated for 3 hours at 37 ° C. During the incubation time, smears were prepared from the cells and stained according to the Rebiger. The smears were prepared after 15, 30 minutes and after 1, 2 and 3 hours. As a result of the work carried out, it was found that the use of amoxicillin in the identification of B. anthracis allows us to obtain the necessary results with a characteristic picture of a "pearl necklace". At the same time, the dynamics of changes in the morphology of cells subjected to incubation in a nutrient medium containing amoxicillin was studied. It was found that changes in the cell wall and cell shape can be observed after 15 minutes of incubation. After 1 hour, the cells underwent significant deformation with the appearance of a large number of separate spherical cell forms. After 2 hours of incubation, the chains of B. anthracis began to acquire the characteristic appearance of a pearl necklace, which was traced after 3 hours.

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Biological Properties of Bacteria Clostridium Perfringens Isolated in the Middle Volga Region From Calves With Anaerobic Enterotoxemia

Cited by 2 publications

References 0 publications

Metagenomic Analysis and Identification of Epizootic Strains of the Causative Agent of Infectious Bovine Keratoconjunctivitis in Kazakhstan

Metagenomic Analysis and Identification of Epizootic Strains of the Causative Agent of Infectious Bovine Keratoconjunctivitis in Kazakhstan

Application of Amoxicillin in the pearl necklace test in the identification of the Anthraxic cause

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