Biological Properties of the Growth Hormonelike Factor from the Plerocercoid of Spirometra mansonoides

Steelman, Sanford L.; Glitzer, Monroe S.; Ostlind, D. A.; Mueller, Justus F.

doi:10.1016/b978-0-12-571127-2.50027-4

Cited by 26 publications

(22 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both cells and crystals were similar in morphology and staining properties to The adenomatous hyperplasia of the fundic portion of the glandular stomach described in the present study in Han:NMRI mice is morphologically similar to that of I strain mice32s41 and differs from lesions in other mouse strains termed chronic gastritis,*O gastric hyperplasia, 18,29, 43 or giant hypertrophic g a~t r i t i s .~~,~~ The latter developed after thymectomy in (C3H/HeMs x 129/F)F, mice due to autoantibodies against parietal cells.24 As a result, the lack of inhibiting factors led to a growth hormone release12 that stimulated the proliferation of gastrin cells,44 and the chronic trophic effect of gastrin on the fundus cells eventually caused an increase in mucosal height.2J2 Several studies have suggested that some of the pathologic changes induced by enteric parasites could be caused by gastrointestinal hormones, particularly g a~t r i n ,~,~~~ mediated possibly by parasite products such as a growth hormone-like factor. 40 In the present study, mice were free of parasites and serum autoantibodies against gastric cells, and parietal cells contributed to the hyperplasia. Gastrin cell counts indicate that an increased chronic gastrin action on the fundus could play a role in the hyperplasia seen in the Han:NMRI mice.…”

Section: Discussionmentioning

confidence: 92%

Spontaneous Nonneoplastic Gastric Lesions in Female Han: NMRI Mice, and Influence of Food Restriction Throughout Life

1987

View full text Add to dashboard Cite

Abstract.A life span study of 900 female virgin Han:NMRI mice (300 outbred controls, 300 of a fat subline, and 300 of a lean subline) was done to determine the occurrence of spontaneous disease with ad libitum or restricted feeding. Major nonneoplastic lesions in the glandular stomach were erosions, ulcers and adenomatous hyperplasia of the fundus. Ulcers, ranging in incidence with ad libitum feeding from 5 to 9% and with food restriction from 13 to 34%, either occurred secondary to other diseases or were due to stress associated with food restriction, causing death mainly in the lean subline. Adenomatous hyperplasia was most common in the control group (4 l0/o); the frequency increased with age and was significantly reduced by food restriction. The etiology of adenomatous gastric hyperplasia is yet unknown. However, a lack of antiparietal cell antibodies, as determined by an immunofluorescent technique, indicates that an autoimmune disorder is not involved and an increase in antral gastrin cells, as determined by avidin-biotin peroxidase immunocytochemistry, suggests a hormonal involvement in pathogenesis.Neoplastic and selected nonneoplastic lesions have been reported on a life span study of food restriction of outbred Han:NMRI mice and two sub line^.^^-^^ Nonneoplastic lesions of the stomach were mainly ulcers of the glandular portion partly due to food r e~t r i c t i o n '~.~~~~~ or in association with physical stress similar to acute stress ulcers in humans, e.g., during prolonged illness.3o Reports of spontaneous gastric ulcers in rats and mice are infrequent, and ulcers are often limited to the f o r e~t o r n a c h .~J~,~~ The predominant gastric lesion of Han:NMRI mice was, however, an adenomatous hyperplasia of the fundus. Of unknown origin, it is comparable to the lesion seen in I strain mice.32g41 Apart from assessment of incidence and influence of food restriction on gastric hyperplasia, the present investigation is concerned with a histomorphologic description of this lesion and with immunohistologic studies that were carried out to discern possible autoimmunez4 and hormonal*J 1~1 2 , 4 4 influences on development. Materials and MethodsA life span study on spontaneous mortality and diseases was camed out on a total of 900 female virgin Han:NMRI mice that were composed of three groups: 300 mice of a fat subline (originally selected for a high body weight at an age of 4 weeks), 300 mice of a lean subline (selected for low body fat content of the parents), and 300 outbred control animals (nonselected). The sublines (1 2th and 13th generation of selection) and controls were obtained from a selection experiment from the Department of Population Genetics and Data Processing of the Central Institute for Laboratory Animal Breeding (Annual Reports of the Central Institute for Laboratory Animals 1975-1 980, Hermann-Ehlers-AIlee 57, D-3000 Hannover, FRG). Animals entered the experiment at an age of 8 weeks. They were kept in one room of a bamertype animal house with 22 & 1 C room temperature, 55 i 5% relativ...

show abstract

Section: Discussionmentioning

confidence: 92%

Spontaneous Nonneoplastic Gastric Lesions in Female Han: NMRI Mice, and Influence of Food Restriction Throughout Life

1987

View full text Add to dashboard Cite

show abstract

“…The pierocercoid larvae of the tapeworm mansonoides release a material, PGF, that induces GH-like effects in adult hosts and suppresses endogenous GH secretion [20]. We have previously shown that infection with S. manso noides in the prepubertal rat is also associated with sup pressed endogenous GH and with a delay in vaginal open ing and ovulation (despite maintenance of normal growth rates) [18].…”

Section: Discussionmentioning

confidence: 99%

“…Unless otherwise indicated, half of the animals in each experi ment were infected at 22 days of age by a single subcutaneous injec tion of 10 plerocercoids suspended in 1 ml of 0.9% saline per rat, thus establishing a chronic plerocercoid infection that stimulates growth in rats [20) and delays puberty onset [18]. In the initial study, one group of controls received an equivalent volume of injection vehicle (I ml of 0.9% saline).…”

Section: Animalsmentioning

confidence: 99%

“…produce a growth factor called plerocercoid growth factor (PGF), that has been shown to stimulate the growth of laboratory rodents and to suppress the synthesis and se cretion of endogenous growth hormone (GH) [20]. In a previous paper, we reported that implantation of S. manso noides at weaning age (22 days of age) results in a signifi cant delay in vaginal opening and first ovulation in the rat [18].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Regulation of Gonadotrophin Secretion in the Prepubertal Period

Ramaley

Phares

1982

Neuroendocrinology

View full text Add to dashboard Cite

The plerocercoid larvae of the tapeworm, Spirometra mansonoides, produce a growth factor, called plerocercoid growth factor (PGF) that suppresses endogenous growth hormone (GH). Treatment (infection) with 10 plerocercoids at weaning age (22 days of age) delayed puberty onset in the female rat. First ovulation was at 34.0 ± 0.6 days in controls and at 38.6 ± 0.9 days in worm-treated rats (p < 0.01). When basal serum hormone levels were measured at 08.00–09.00 h at several times during the prepubertal period, no changes were found in FSH, LH or prolactin. Growth hormone (GH) was below measurable levels in both groups before day 27. GH of infected rats remained significantly suppressed at least until the second metestrus. Thyroxine (T₄) was significantly lower in worm-treated animals beginning at 26 days of age and remained depressed until 33 days of age. Gonadotrophin regulation was assessed by measuring the rate of rise of serum FSH and LH during the first 48 h after castration at 25, 28 and 32 days of age and on the day of the second metestrus. The rate of increase in FSH fell with age in both groups but was significantly less at all prepubertal ages in worm-treated rats. The LH response was less in castrated postpubertal controls but there was not a clear-cut progressive fall with age. The LH response was less in worm-treated rats at all the prepubertal ages tested but not at second metestrus. To determine whether the lack of difference between the two metestrus groups was due to a diminution of the worm effect, worms were implanted at 30 days of age and the castration response at second metestrus was compared to that obtained in rats given worms at 22 days of age. The FSH and LH responses in both sets of worm-treated rats were comparable, indicating that the loss of suppression relates to age rather than a decreased secretion of PGF. To determine whether the acute suppression of the castration response would persist, measurements of serum FSH and LH were made 4, 7 and 14 days after castration at 25 days of age. By 4 days after castration, FSH and LH were comparable in the two groups. This occurred despite the continuation of T4 and GH deficiency at that age. When the castration response was suppressed by the daily administration of 0.05 or 0.5 μg estradiol benzoate (EB), worm-treated animals showed a greater sensitivity to both doses of EB. Delay in puberty onset in worm-treated animals is associated with both T4 and GH deficiency but the suppression of the response of FSH and LH to castration and the heightened sensitivity to estrogen are not attributable solely to these deficits.

show abstract

“…The latter species, in particular, has a growth-promoting effect on several species of rodents in a manner analogous to the action of growth hormone (GH) (Mueller, 1963(Mueller, , 1965(Mueller, , 1968Steelman, Glitzer, Ostlind & Mueller, 1971), by increasing the adipose tissue weight of its host. This lipogenic effect constitutes the only difference from the effect of GH.…”

Section: Introductionmentioning

confidence: 99%

Lipid metabolism in golden hamsters infected with plerocercoids ofSpirometra erinacei(Cestoda: Pseudophyllidea)

Tsuboi

Hirai

1986

Parasitology

View full text Add to dashboard Cite

Golden hamsters infected with Spirometra erinacei plerocercoids develop a hypertriglyceridaemia characterized by an increase in very low-density lipoprotein. Acyl-CoA synthetase activity, the rate of fatty acid synthesis in the liver and triglyceride production studies showed that the activation and synthesis of fatty acid and production of triglyceride in plerocercoid-infected hamsters were not significantly different from the controls. Lipase activity in post-heparin plasma was found to be suppressed in plerocercoid-infected golden hamsters. The presence of plerocercoids also resulted in a significant reduction in serum thyroxine, but the hypertriglyceridaemia associated with plerocercoid infection was not reversed by injecting the infected hamsters with 2 micrograms/day doses of L-thyroxine. The levels of serum immunoreactive insulin in plerocercoid-infected hamsters were not significantly different from the controls. We conclude that the hypertriglyceridaemia, associated with plerocercoid infection in hamsters, results predominantly from a suppression of triglyceride degradation, and that the suppression of lipase activity is probably not the result of hypothyroidism or the lack of insulin, but the result of secretion of growth hormone-like substances.

show abstract

Biological Properties of the Growth Hormonelike Factor from the Plerocercoid of Spirometra mansonoides

Cited by 26 publications

References 23 publications

Spontaneous Nonneoplastic Gastric Lesions in Female Han: NMRI Mice, and Influence of Food Restriction Throughout Life

Spontaneous Nonneoplastic Gastric Lesions in Female Han: NMRI Mice, and Influence of Food Restriction Throughout Life

Regulation of Gonadotrophin Secretion in the Prepubertal Period

Lipid metabolism in golden hamsters infected with plerocercoids ofSpirometra erinacei(Cestoda: Pseudophyllidea)

Contact Info

Product

Resources

About