Biological properties of viral haemorrhagic septicaemia (VHS) virus: Adsorption and replication

Steps of the Replication Cycle of the Viral Haemorrhagic Septicaemia Virus (VHSV) Affecting Its Virulence on Fish

“…Only in two cases (two low virulent strains, DC1412 [L] in EPC and DK1p8 [L] in RTG-2) EOA values were around 90%; it would be interesting to study if any change in the glycoprotein in these strains could affect the conformational processes leading to internalization and making the adsorption step irreversible, but it was not the aim of our study. In addition, in their report, Frost et al [37] obtained the highest viral titers at 18 h p.i., which does not coincide with what we have observed, probably because we have not worked with single-cycle replication assays.…”

“…Based on the results of Frost et al [37], a 1-h adsorption time was enough to reach a maximum 90% of adsorption to the cells. Those levels of adsorption have been reported in VHSV by other authors [38].…”

“…Few studies on the kinetics of VHSV replication in cell culture have been reported, and many of them with time intervals of 24 h, good enough to study the kinetics of viral replication, but not to analyze the synthesis phase, which is very important to understand the effectiveness of the viral cycle and the effect of any intrinsic or external factor on the virus. This is because the virus must need less than 18 h to complete a replication cycle, based on a very early report by Frost et al [37], which set the stage for understanding the replication process of VHSV in cell lines (RTG-2 and FHM), from the viral adsorption to synthesis kinetics and progeny production. Inspired by this work, we have designed our experiments in order to assess the efficiency of VHSV replication at all the steps involved in this process, to identify the mechanisms possibly involved in VHSV virulence.…”