This paper presents results obtained from a performance study on the biotreatment of 1,3-butadiene in an air stream using a reactor that consisted of a two-stage, inseries biotrickling filter connected with a three-stage, inseries biofilter. Slags and pig manure-based media were used as packing materials for the biotrickling filter and the biofilter, respectively. Experimental results indicated that, for the biotrickling filter portion, the butadiene elimination capacities were below 5 g/m /hr. The elimination rates of butadiene in both the biotrickling filter and biofilter were mass-transfer controlled for influent butadiene concentrations below about 600 ppm for superficial gas velocities of 29-142 m/hr. The elimination capacity was significantly higher in the biofilter than in the biotrickling filter. This discrepancy may be attributed to the higher IMPLICATIONS 1,3-butadiene is frequently used for manufacturing synthetic rubber and resin and can be released from processing equipment and ducts. It is one of the 189 hazardous air pollutants listed in the 1990 Clean Air Act Amendments. For treating such volatile organic compounds, biofilters and biotrickling filters are efficient. However, to our knowledge, the use of either approach for the biological treatment of 1,3-butadiene has not been investigated. This work analyzes the performance of biofilters and biotrickling filters for treating this target waste compound. The paper also provides data on the approximate elimination capacities and appropriate operating conditions for the biotreatment of olefins with low molecular weight.mass-transfer coefficient and gas-solid interfacial area offered for transferring the gaseous butadiene in the biofilter.