2013
DOI: 10.1007/s12010-013-0187-1
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Biological Removal of Phosphate at Low Concentrations Using Recombinant Escherichia coli Expressing Phosphate-Binding Protein in Periplasmic Space

Abstract: During wastewater treatment, phosphate removal is an important and challenging process; thus, diverse technologies, including those derived from biological means, have been devised for efficient phosphate removal. Although conventional biological methods are effective in decreasing wastewater phosphate levels to ~1 mg/L, long periods of microbial adaptation are required for effective phosphate removal, and the removal efficiency of these methods is relatively poor at lower phosphate concentrations. In the pres… Show more

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Cited by 14 publications
(9 citation statements)
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“…15,16 Removal of P i to ultralow concentrations has been demonstrated using PBP expressed in bacterial cells' periplasmic space, expressed on the cells' surface, or immobilized on Sepharose beads. [10][11][12]14,17,18 Beyond P i removal, Venkiteshwaran et al (2018) 14 investigated pH and temperature conditions to induce controlled P i desorption from immobilized PBP. They demonstrated that high pH conditions (pH 12.5) released >90% of P i adsorbed on immobilized PBP.…”
Section: Introductionmentioning
confidence: 99%
“…15,16 Removal of P i to ultralow concentrations has been demonstrated using PBP expressed in bacterial cells' periplasmic space, expressed on the cells' surface, or immobilized on Sepharose beads. [10][11][12]14,17,18 Beyond P i removal, Venkiteshwaran et al (2018) 14 investigated pH and temperature conditions to induce controlled P i desorption from immobilized PBP. They demonstrated that high pH conditions (pH 12.5) released >90% of P i adsorbed on immobilized PBP.…”
Section: Introductionmentioning
confidence: 99%
“…Removal of P i to ultra-low concentrations has been demonstrated using PBP expressed in bacterial cells' periplasmic space, expressed on the cells' surface, or immobilized on Sepharose beads (Choi et al., 2013; Kuroda et al., 2000; Li et al., 2009; Yang et al., 2016). For example, Choi et al. (2013) showed that recombinant E. coli expressing PBP in the periplasmic space can remove ≥97% P i from water within 6 h (initial concentration of 0.2–0.5 mg-P i /L).…”
Section: Introductionmentioning
confidence: 99%
“…The suspensions were mixed on an orbital shaker at room temperature (22°C) for 3 h. Choi et al (2013) reported efficient removal of P using E. coli in 6-h batch-scale adsorption tests, indicating that cell integrity was maintained throughout the 3 h test used in this study. We also confirmed integrity of the cell using the Bradford assay, which indicated that the concentration of proteins released from the cells after 3 h was below the detection limit of 0.125 mg L −1 .…”
Section: Unexpressed Controls and P Analysismentioning
confidence: 79%