Biological responses of T cells encapsulated with polyelectrolyte-coated gold nanorods and their cellular activities in a co-culture system

Wattanakull, Porntida; Killingsworth, Murray C.; Pissuwan, Dakrong

doi:10.1007/s13204-017-0605-8

Cited by 6 publications

(2 citation statements)

References 38 publications

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“…This cell line has been widely used in studying immune responses, signaling pathways, and nutrient and drug transport while cells are in the monocyte state as well as in the macrophage state . Encapsulation of immune cells, such as THP-1 cells, has often been done in immunotherapy and related experimental applications. − Such cellular encapsulation can shield immune cells from the host’s immune response, which can reduce the risk of rejection and improve the longevity of the transplanted cells particularly in cases where the immune cells are modified or engineered for therapeutic purposes. Additionally, encapsulation can provide a controlled environment that can help to maintain the function and phenotype of the encapsulated immune cells.…”

Section: Discussionmentioning

confidence: 99%

“…Their ease of culture and maintenance highlight their suitability for various experimental designs. − These factors collectively prompted us to choose THP-1 cells as our model for the nanocoating study. Encapsulation of immune cells is often utilized in immunotherapy and related experimental applications. − Such encapsulation can shield immune cells from the host’s immune response, particularly in cases where the immune cells are modified or engineered for therapeutic purposes. This protection can be crucial for the survival and functional efficacy of the transplanted or modified cells in experimental and clinical settings.…”

Section: Introductionmentioning

confidence: 99%

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Impact of Silk-Ionomer Encapsulation on Immune Cell Mechanical Properties and Viability

Kumarasinghe,

Hasturk,

Wang

et al. 2024

ACS Biomater. Sci. Eng.

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Encapsulation of single cells is a powerful technique used in various fields, such as regenerative medicine, drug delivery, tissue regeneration, cell-based therapies, and biotechnology. It offers a method to protect cells by providing cytocompatible coatings to strengthen cells against mechanical and environmental perturbations. Silk fibroin, derived from the silkworm Bombyx mori, is a promising protein biomaterial for cell encapsulation due to the cytocompatibility and capacity to maintain cell functionality. Here, THP-1 cells, a human leukemia monocytic cell line, were encapsulated with chemically modified silk polyelectrolytes through electrostatic layer-by-layer deposition. The effectiveness of the silk nanocoating was assessed using scanning electron microscopy (SEM) and confocal microscopy and on cell viability and proliferation by Alamar Blue assay and live/dead staining. An analysis of the mechanical properties of the encapsulated cells was conducted using atomic force microscopy nanoindentation to measure elasticity maps and cellular stiffness. After the cells were encapsulated in silk, an increase in their stiffness was observed. Based on this observation, we developed a mechanical predictive model to estimate the variations in stiffness in relation to the thickness of the coating. By tuning the cellular assembly and biomechanics, these encapsulations promote systems that protect cells during biomaterial deposition or processing in general.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Impact of Silk-Ionomer Encapsulation on Immune Cell Mechanical Properties and Viability

Kumarasinghe,

Hasturk,

Wang

et al. 2024

ACS Biomater. Sci. Eng.

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Temporary Nanoencapsulation of Human Intestinal Organoids Using Silk Ionomers

Wang,

Hasturk,

Kumarasinghe

et al. 2024

Adv Healthcare Materials

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Human intestinal organoids (HIOs) are vital for modeling intestinal development, disease, and therapeutic tissue regeneration. However, their susceptibility to stress, immunological attack, and environmental fluctuations limits their utility in research and therapeutic applications. This study evaluated the effectiveness of temporary silk protein‐based layer‐by‐layer (LbL) nanoencapsulation technique to enhance the viability and functions of HIOs against common biomedical stressors, without compromising their native functions. Cell viability and differentiation capacity are assessed, finding that nanoencapsulation significantly improved HIO survival under the various environmental perturbations studied without compromising cellular functionality. Post‐stress exposures, the encapsulated HIOs still successfully differentiated into essential intestinal cell types such as enterocytes, goblet cells, enteroendocrine cells, and Paneth cells. Moreover, the silk nanocoatings effectively protected against environmental stressors such as ultraviolet (UV) light exposure, protease degradation, antibody binding, and cytokine‐induced inflammation. This nanoencapsulation technique shows promise for advancing HIO applications in disease modeling, drug testing, and potential transplantation therapies.

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Breast Cancer Cell Destruction Using Individually Encapsulated Cytotoxic T Lymphocytes in Polyelectrolyte Layer Coated with Dual Nanoparticles in Combination with Magnetic Exposure and Laser Irradiation

Wattanakull,

Pissuwan

2024

Nano Select

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Cell encapsulation can protect cells from the host immune system. It is well‐known that cytotoxic T lymphocytes (CTLs) can destroy cancer cells. In this study, we individually encapsulated CTLs in a cationic polyelectrolyte in combination with two types of nanomaterials: magnetic nanoparticles (MNPs) and gold nanorods (GNRs) conjugated to anti‐human epidermal growth factor receptor‐2 (HER2) antibodies referred to as CTLs/mMNPs/PAH/GNRs@HER2. When MCF‐7 breast cancer cells were co‐cultured with CTLs/mMNPs/PAH/GNRs@HER2 and subjected to a magnetic field and laser irradiation, the viability of MCF‐7 cells was significantly decreased to 42.98 ± 0.57% in comparison to MCF‐7 cells without any treatment or MCF‐7 cells co‐cultured with CTLs (51.52 ± 0.57%). This can be a novel strategy for cancer treatment using encapsulated CTLs in polyelectrolytes and dual nanomaterials in combination with magnetic exposure and laser irradiation.

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Biological responses of T cells encapsulated with polyelectrolyte-coated gold nanorods and their cellular activities in a co-culture system

Abstract: Currently, human T cell therapy is of considerable scientific interest. In addition, cell encapsulation has become an attractive approach in biomedical applications.

Cited by 6 publications

References 38 publications

Impact of Silk-Ionomer Encapsulation on Immune Cell Mechanical Properties and Viability

Impact of Silk-Ionomer Encapsulation on Immune Cell Mechanical Properties and Viability

Temporary Nanoencapsulation of Human Intestinal Organoids Using Silk Ionomers

Breast Cancer Cell Destruction Using Individually Encapsulated Cytotoxic T Lymphocytes in Polyelectrolyte Layer Coated with Dual Nanoparticles in Combination with Magnetic Exposure and Laser Irradiation

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