Biological roles of angiotensin ii via its type 2 receptor during rat follicle atresia

Kotani, Eri; Kondo, Koichi; Saitoh, Masahiro; Usuki, Satoshi; Kubo, Takao; Song, Keifu; Miyazaki, Masao; Miyazaki, Hideto

doi:10.1016/s0020-7292(00)84681-0

Cited by 4 publications

(9 citation statements)

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“…Thereafter, the cells were stimulated for the indicated periods. FSH at 200 ng/ml significantly activated both ERK1/2 and p38, which was consistent with findings in rat and porcine granulosa cells (Babu et al 2000;Cameron et al 1996;Das et al , 1996: Gebauer et al , 1999Kotani et al , 1999;Maizels et al , 1998). Serum (10%) and H 2 O 2 at 100 µ M also increased ERK1/2 and p38 activities within two minutes.…”

Section: Activation Of Erk and P38 By Fsh Serum And H 2 Osupporting

confidence: 89%

“…These findings suggest that the determination of the survival and apoptosis of granulosa cells is also critically regulated by ERK and p38. However, there are few reports, including ours, indicating the activation of ERK and p38 in these cells (Babu et al , 2000;Cameron et al , 1996;Das et al , 1996: Gebauer et al , 1999Kotani et al , 1999;Maizels et al , 1998). Moreover, no direct evidence has confirmed the involvement of these two kinases in the processes of survival and apoptosis of primary cultured granulosa cells.…”

Section: Introductionmentioning

confidence: 63%

“…Several reports indicated that FSH elicits ERK activation in rat and porcine primary cultured granulosa cells (Cameron et al, 1996;Das et al, 1996;Kotani et al, 1999). However, none of them showed direct evidence for the involvement of ERK in regulating granulosa cell survival or apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…We converted the weight of NIDDK-oFSH-20 into that of NIDDK-oFSH-15, the potency of which is 20-fold greater than that of NIH-FSH-S1. NIDDK-oFSH-15 has been widely applied in many studies of the ovary including ours (Kotani et al , 1999). Fetal bovine serum, L-glutamine, and the antibiotics, penicillin and streptomycin, were purchased from Sigma Chemical Co. (St. Louis, MO, USA).…”

Section: Reagentsmentioning

confidence: 99%

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Correlation of Mitogen-Activated Protein Kinase Activities with Cell Survival and Apoptosis in Porcine Granulosa Cells

et al. 2003

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The regulation of granulosa cell survival and death is critical for determining the fate of ovarian follicles. Mitogen-activated protein kinases (MAPKs) play central roles in various cellular responses, but the relationship between MAPK activities and granulosa cell survival as well as death is poorly understood. The present study examines the roles of the extracellular signal-regulated kinase (ERK) and p38 MAPK activities in porcine granulosa cells in response to survival factors and oxidative stress. Cell survival and apoptosis were evaluated by Trypan blue staining, DNA fragmentation, and chromatin staining with Hoechst 33342. Cell survival induced by serum or by follicle-stimulating hormone (FSH) was inhibited when ERK activity was attenuated with PD98059, which led to the induction of apoptosis. The p38 inhibitor SB203580 significantly decreased the cell survival evoked by FSH, but not by serum. Even in the presence of 10% serum, H(2)O(2) caused apoptosis, indicating that H(2)O (2) may be an atretogenic factor or its mediator. Interestingly, this induction of apoptosis was also prevented by SB203580, suggesting that p38 is involved in an apoptotic pathway induced by H(2)O (2) as well as in a survival pathway evoked by FSH in granulosa cells. These results indicate that whereas ERK activity is critical to the survival of granulosa cells, p38 activity contributes to their survival or apoptosis depending on the stimulus.

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Section: Activation Of Erk and P38 By Fsh Serum And H 2 Osupporting

confidence: 89%

Section: Introductionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Reagentsmentioning

confidence: 99%

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Correlation of Mitogen-Activated Protein Kinase Activities with Cell Survival and Apoptosis in Porcine Granulosa Cells

et al. 2003

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“…However, the timing of AT2R expression during oocyte maturation is uncertain and varies between species. Indeed, a study using autoradiography and gene expression measurements reported the expression of AT2R in granulosa cells of rat atretic follicles while it is almost absent in healthy follicles [27]. In contrast, studies in bovine ovaries demonstrate that AT2R expression is increased during follicular growth and maturation [15].…”

Section: Ras and Ovary And Follicular Developmentmentioning

confidence: 99%

Role of the Renin-Angiotensin System in Healthy and Pathological Pregnancies

Pepin¹,

Dehboneh²,

Raguema³

et al. 2017

Renin-Angiotensin System - Past, Present and Future

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Introduction: Pregnancy is a physiological process that necessitates many cardiovascular and hemodynamic adaptations to ensure the survival of the foetus and well-being of the mother. The renin-angiotensin system (RAS) has been suggested as key player in many of these changes as it is critical for blood pressure control as well as fluid and salt homeostasis in the non-pregnant state. Body: Normal pregnancy is characterized by an increase in the circulating levels of prorenin, renin, angiotensinogen and angiotensin-II. However, this is coupled to a diminished endothelial sensitivity to angiotensin-II, which may explain the lack of increase in blood pressure in pregnancy. Conversely, an increase in circulating levels of aldosterone and anti-diuretic hormone during pregnancy can be observed and could contribute to the enhanced renal sodium and water reabsorption, respectively. Moreover, dysregulation of the RAS has been implicated in the development of gestational hypertensive disorders such as preeclampsia. Conclusion: The difference in the RAS effects observed during normal pregnancy may be attributable to local modifications of the RAS as well as to non-classic RAS such as the angiotensin-(1-7) axis. These adaptations may be dysregulated during preeclampsia and may contribute to the development of the disease.

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Immunocytochemical localization of angiotensin II receptor subtypes 1 and 2 in the porcine fetal, prepubertal and postpubertal ovary

et al. 2002

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There is considerable evidence for a mammalian ovarian renin-angiotensin system, which may influence ovulation, angiogenesis and steroidogenesis via the autocrine and/or paracrine actions of the biologically active product of the cascade, angiotensin II (AngII). There are two characterized AngII receptors -type 1, AT 1 and type 2, AT 2 .We report the localization of these receptor subtypes within porcine fetal, prepubertal and postpubertal ovaries. Positive staining for AT 1 and AT 2 receptors was observed in egg nests in all fetal ovaries studied, as well as in a defined two-cell layer at the ovarian periphery. In prepubertal tissue, positive AT 1 and AT 2 staining was localized to granulosa cells adjacent to the basement membrane of pre-antral and antral follicles, with no staining in the thecal layer. There was immunostaining for both receptors in prepubertal oocytes and zona pellucida. In postpubertal tissue, positive AT 1 and AT 2 immunostaining was localized to areas of putative neovascularization, the zona pellucida and the oocyte. Further AT 1 staining was located to the postpubertal antral follicle granulosa cells. The results indicate that there are higher densities of AT 1 receptors than AT 2 receptors in the porcine fetal, prepubertal and postpubertal ovary, and this has profound implications for the role of AngII in ovarian development.

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Biological roles of angiotensin ii via its type 2 receptor during rat follicle atresia

Cited by 4 publications

References 0 publications

Correlation of Mitogen-Activated Protein Kinase Activities with Cell Survival and Apoptosis in Porcine Granulosa Cells

Correlation of Mitogen-Activated Protein Kinase Activities with Cell Survival and Apoptosis in Porcine Granulosa Cells

Role of the Renin-Angiotensin System in Healthy and Pathological Pregnancies

Immunocytochemical localization of angiotensin II receptor subtypes 1 and 2 in the porcine fetal, prepubertal and postpubertal ovary

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