Biological screening of Ajuga iva extracts obtained by supercritical carbon dioxide and pressurized liquid extraction

Bendıf, Hamdi; Lazali, Mohamed; Harir, Mohammed; Mıara, Mohamed Djamel; Boudjeniba, Messaoud; Venskutonis, Petras Rimantas

doi:10.25081/jmb.2017.v1.816

Cited by 9 publications

(6 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, from the obtained results for all extracts under study, the most suitable extractor solvents were water and ethanol. The obtained results are in accordance with those found by several studies (Bendif et al, 2017;El-lamey, 2022;Fettach et al, 2019;Makni et al, 2013;Salem et al, 2016;Senhaji et al, 2020). Bioactive compounds are synthesized in different structures and chemical natures which affect their extraction (Joana Gil-Chávez et al, 2013).…”

Section: Discussionsupporting

confidence: 92%

Impact of Geographical Conditions on Phenolic and Flavonoid Contents and Antioxidant Activity of Different Extracts of Ajuga iva

Lahrizi,

Errachidi,

Ghadraoui

2024

IJARe

View full text Add to dashboard Cite

In the present investigation, various cultivars of Ajuga iva were collected from different locations in Morocco in 2022. These cultivars were subjected to extraction using different solvents, namely water, ethanol and methanol, to obtain extracts from their aerial parts. The extracts were then analyzed to determine their total phenolic content, total flavonoid content, total sugar content, hydrolysable tannin content, condensed tannin content and antioxidant activity. The results obtained from the analysis revealed the following quantities for the different parameters: The total phenolic content ranged from 226.04±8.47 to 22.59±2.43 mg GAE/g dw, the total flavonoid content ranged from 22.27±0.11 to 3.35±0.006 mg QE/g dw, the total sugar content ranged from 38.78±2.56 to 2.88±0.18 mg/g dw, the reducing sugar content ranged from 7.17±0.45 to 0.41±0.007 mg/g dw, the hydrolysable tannin content ranged from 12.25±0.017 to 0.75±0.15 mg TAE/g dw, the condensed tannin content ranged from 25.49±0.53 to 3.35±1.85 mg/g dw and the total antioxidant capacity ranged from 0.18±0.012 to 0.010±0.004 mg AAE/g dw. Furthermore, a principal component analysis was conducted to assess the relationship between the different parameters. The analysis revealed a strong correlation between the total phenolic content, total flavonoid content and hydrolysable tannin content with the total antioxidant capacity. This suggests that these compounds contribute significantly to the antioxidant capacity of Ajuga iva. Overall, the findings of this study demonstrate that Ajuga iva contains substantial amounts of bioactive compounds and possesses a noteworthy antioxidant capacity. These results contribute to the understanding of the chemical composition and potential health benefits of Ajuga iva.

show abstract

Section: Discussionsupporting

confidence: 92%

Impact of Geographical Conditions on Phenolic and Flavonoid Contents and Antioxidant Activity of Different Extracts of Ajuga iva

Lahrizi,

Errachidi,

Ghadraoui

2024

IJARe

View full text Add to dashboard Cite

show abstract

“…These results agree with several similar studies, which showed that aqueous extracts showed higher yields than extracts obtained by organic solvents [ 81 , 82 , 83 ]. For example, extraction yields determined from extracts of A. iva (L.) obtained by acetone, ethanol, and water were respectively around 2.3%, 2.6%, and 24% [ 19 ]. Additionally, the extraction yield seems to be influenced by the degree of polarity of the solvent, the extraction method, the extraction time, as well as by the degree of polarity of the various components of the extract, such as the phenolic constituents [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

“…This plant species has a number of slang names, including Chendghoura and Toutoulba [ 18 ]. Some Ajuga species are regarded as weeds, yet many are employed in horticulture as ground or border cover and in rock gardens [ 19 ]. In Morocco, many ethno-medical uses have been reported for this plant.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Profile, Antioxidant, Antimicrobial, and Antidiabetic Activities of Ajuga iva (L.)

Saidi

Remok

Handaq

et al. 2023

Life

View full text Add to dashboard Cite

In Morocco, many applications in ethnomedicine on Ajuga iva (L.) have been recognized as able to treat various pathologies such as diabetes, stress, and microbial infections. The objective of this work is to carry out phytochemical, biological, and pharmacological investigations on the extracts of Ajuga iva leaves in order to confirm its therapeutic effects. The phytochemical screening carried out on the different extracts of Ajuga iva showed its richness in primary (lipids and proteins) and secondary metabolites (flavonoids, tannins, reducing compounds, oses, and holoside. The best contents of polyphenols, flavonoids, and tannins evaluated by spectrophotometric methods were found in the hydroethanolic extract (69.850 ± 2.783 mg EAG/g DE, 17.127 ± 0.474 mg EQ/g DE, 5.566 ± 0.000 mg EQC/g DE), respectively. Analysis of the chemical composition of the aqueous extract by LC/UV/MS revealed 32 polyphenolic compounds including ferulic acid (19.06%), quercetin (10.19%), coumaric acid (9.63%), and apigenin-7-(2-O-apiosylglucoside) (6.8%). The antioxidant activity of Ajuga iva extracts was evaluated by three methods (DPPH*, FRAP, CAT). The hydroethanolic extract recorded the strongest reducing power: DPPH* (IC50 = 59.92 ± 0.7 µg/mL), FRAP (EC50 = 196.85 ± 1.54 (µg/mL), and CAT (199.21 ± 0.37 mg EAG/gE). A strong correlation between phenolic compounds and antioxidant activities was confirmed by the determination of Pearson’s coefficient. The antimicrobial activity of Ajuga iva studied by the microtiter method revealed potent antifungal and antibacterial qualities against Candida parapsilosis and Staphylococcus aureus BLACT. An in vivo oral glucose tolerance test (OGTT) using normal rats revealed that the antihyperglycemic action of the aqueous extract significantly reduced postprandial hyperglycaemia at (30 min, p < 0.01) and area under the curve (AUC glucose), p < 0.01. Similarly, the aqueous extract, tested on pancreatic α-amylase enzyme activity in vitro and in vivo significantly inhibited pancreatic α-amylase activity with IC50 = 1.52 ± 0.03 mg/mL. In conclusion, the extract from Ajuga iva could be a good source of bioactive molecules, which exhibit potent antioxidant and antimicrobial activity, as well as strong antidiabetic activity, for applications in the pharmaceutical industry.

show abstract

“…Makni et al [ 9 ] observed that the extraction yield of A. iva differed between methanol, aqueous, hexane, and chloroform extractions. Bendif et al [ 10 ] observed different concentrations of total phenolics and free radical-scavenging activity with different extraction methods (acetone, ethanol, and water) of A. iva .…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition, Antioxidant, and Antimicrobial Activities of Leaves of Ajuga Iva

Ammar

Touihri²,

Kholif

et al. 2022

Molecules

View full text Add to dashboard Cite

The main objective of this research was to study the biological characteristics in terms of antioxidant and antimicrobial activities of Ajuga iva and determine the best analytical and extraction methods applicable to this specie and studied compounds. A short screening of its nutritional value in terms of chemical composition is also included. A. iva leaves were analyzed for crude protein (CP), cell wall [neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL)], minerals, fatty acids, essential oils, and phenolic compounds. Mature aerial parts of A. iva were randomly collected during the Spring season from Mograne-Zaghouan, Tunisia. Leaves of A. iva contained 13.4 ± 0.4% CP, 26.3 ± 0.35% NDF, 20.2 ± 0.42% ADF, and 5.13 ± 0.21% ADL. Mineral content (13.0 ± 0.45%) was mainly composed of potassium (4.5% g DM) and magnesium (4.25% DM). Leaves of A. iva had linolenic (26.29 ± 0.760%) and linoleic (37.66 ± 2.35%) acids as the main components of the acid profile. Thymol was found to be the most dominant (23.43%) essential oil, followed by 4-vinylguaiacol (14.27%) and linalool (13.66%). HPLC-PDA-ESI-MS/MS analysis pointed out the presence of phytoecdysteroids. Phenolic acids and flavonoids, such as glycosylated derivatives of naringenin, eriodyctiol, and apigenin, were detected in the methanol extract of A. iva leaves. Our results underline the importance of choosing proper extraction methods and solvents to extract and characterize the described compounds profile of A. iva leaves. Results also show A. iva leaves as a potential source of functional ingredients with beneficial health-promoting properties. Overall, leaves of A. iva have low biological activities (antioxidant and antimicrobial activities) with a chemical composition suitable as a feed for ruminants in rangeland pasture. It also has low-grade antibacterial or medicinal characteristics when fed to ruminants.

show abstract

Biological screening of Ajuga iva extracts obtained by supercritical carbon dioxide and pressurized liquid extraction

Cited by 9 publications

References 19 publications

Impact of Geographical Conditions on Phenolic and Flavonoid Contents and Antioxidant Activity of Different Extracts of Ajuga iva

Impact of Geographical Conditions on Phenolic and Flavonoid Contents and Antioxidant Activity of Different Extracts of Ajuga iva

Phytochemical Profile, Antioxidant, Antimicrobial, and Antidiabetic Activities of Ajuga iva (L.)

Chemical Composition, Antioxidant, and Antimicrobial Activities of Leaves of Ajuga Iva

Contact Info

Product

Resources

About