Biological, Serological and Molecular Diagnosis of Three Major Potato Viruses in Egypt

El-Araby, W.S.; Ibrahim, Imad; Hemeida, Alaa A.; Mahmoud, Amal; Soliman, A.M.; El-Attar, Ahmed K.; Mazyad, H. M.

doi:10.3923/ijv.2009.77.88

Cited by 21 publications

(10 citation statements)

References 21 publications

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“…White Burley plants and showed mosaic on leaves after one month of incubation period under greenhouse conditions. Similar results were also recorded by El-Araby et al (2009). The previously obtained PVX was mechanically transmitted to healthy potato plants ( Fig.…”

Section: Results and Discussion Isolation And Identification: As Showsupporting

confidence: 89%

Antiviral Activity of Lactoferrin against Potato virus x In vitro and In vivo

Taha¹,

Mokbel²,

Abdel-Hamid³

et al. 2015

International J. of Dairy Science

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Potato is one of the most important food crops after wheat and rice. The effectiveness of lactoferrin against Potato virus x (PVX) in vitro and in vivo have been evaluated. Four concentrations of lactoferrin 100, 250, 500 and 1000 mg LG 1 were examined either in vitro culture medium or in vivo (greenhouse). The presence of the virus was evaluated by ELISA technique. Data demonstrated that application of 1000 mg LG 1 lactoferrin by spraying or combined with tissue culture proved to be an effective method for PVX-inhibition as compared with other concentrations. Also, results of antiviral activity of lactoferrin at concentration 1000 mg LG 1 showed great potential as phytotherapeutic source to produce quality and health plantlets for rapid and large scale in vitro production. Lactoferrin seemed to be very successful and inexpensive in controlling viral infection through in vitro technique or by spraying the infected and/or healthy plant under greenhouse condition.

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Section: Results and Discussion Isolation And Identification: As Showsupporting

confidence: 89%

Antiviral Activity of Lactoferrin against Potato virus x In vitro and In vivo

Taha¹,

Mokbel²,

Abdel-Hamid³

et al. 2015

International J. of Dairy Science

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“…Many techniques have been developed and used for the diagnosis of potato viral diseases including PVX ( El-Araby et al, 2009 ; Khan et al, 2003 ). ELISA has been accepted as the most common and reliable detection method for viruses including PVX because it has long history of use and it is also rapid and inexpensive ( Clark and Adams, 1977 ; Makkouk and Kumari, 2006 ).…”

Section: Discussionmentioning

confidence: 99%

“…Several diagnostic methods have been developed for the diagnosis of viral diseases in potatoes ( El-Araby et al, 2009 ). Major potato viruses such as PVA, PVS, PVX, PVY, and PLRV can be detected at the same time by employing multiplex RT-PCR ( Wang et al, 2005 ).…”

mentioning

confidence: 99%

Development of a Rapid Detection Method for Potato virus X by Reverse Transcription Loop-Mediated Isothermal Amplification

Jeong

Cho

Lee

et al. 2015

The Plant Pathology Journal

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The primary step for efficient control of viral diseases is the development of simple, rapid, and sensitive virus detection. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been used to detect viral RNA molecules because of its simplicity and high sensitivity for a number of viruses. RT-LAMP for the detection of Potato virus X (PVX) was developed and compared with conventional reverse transcription polymerase chain reaction (RT-PCR) to demonstrate its advantages over RT-PCR. RT-LAMP reactions were conducted with or without a set of loop primers since one out of six primers showed PVX specificity. Based on real-time monitoring, RT-LAMP detected PVX around 30 min, compared to 120 min for RT-PCR. By adding a fluorescent reagent during the reaction, the extra step of visualization by gel electrophoresis was not necessary. RT-LAMP was conducted using simple inexpensive instruments and a regular incubator to evaluate whether RNA could be amplified at a constant temperature instead of using an expensive thermal cycler. This study shows the potential of RT-LAMP for the diagnosis of viral diseases and PVX epidemiology because of its simplicity and rapidness compared to RT-PCR.

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“…Absence of PVA and PVM in farm saved seed tubers after detection using DAS-ELISA is not conclusive enough to ascertain that the samples were free from these two viruses. Studies have suggested use of molecular techniques such as Polymerase Chain Reaction (PCR) alongside DAS-ELISA so as to detect viruses even under low concentrations [66].…”

Section: Physical Quality and Infection With Tuber-borne Disease Pathmentioning

confidence: 99%

Seed Potato Production Practices and Quality of Farm Saved Seed Potato in Kiambu and Nyandarua Counties in Kenya

Mumia¹,

Muthomi²,

Narla³

et al. 2018

WJAR

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Potato production in Kenya is mainly constrained by limited supply of quality seed potato tubers. The objective of this study was to determine seed potato handling practices and quality of farm saved seed potato. A survey involving 79 farmers was conducted in potato production areas in Kiambu and Nyandarua Counties to collect information on seed potato production practices including sources of seed tubers, seed selection, seed tuber storage, pests and diseases. Samples of seed potato were collected from farmers and evaluated for quality parameters such as tuber size, weight, stout sprout length and infection with diseases. Factors affecting potato production included limited quality seed supply, pests and diseases. Shangi was the main potato variety grown by majority (62.8%) of farmers and all the farmers used own farm saved seed. Most (45.5%) farmers recycled the seed for four seasons and 44.1% of farmers stored seed potato for three months. Freeness from seedborne diseases was the main seed quality preference by farmers. The main pest reported was cutworms (42.6% of farmers) while potato late blight and bacterial wilt were the major diseases reported by 87.1% and 86.7% of the farmers respectively. All farm saved seed potato samples were infected with Fusarium coeruleum, 51.2% with Rhizoctonia solani, 53.3% with Fusarium sambunicum, 33.3% with Fusarium solani and 48.3% with Aspergillus niger. Over 69% of the farm saved seed samples were infected with Ralstonia solanacearum while 40.1% were infected with Potato Virus S, the most dominant. Due to poor post-harvest handling practices, farmers incurred seed quality and quantity losses in storage. Farm saved seed is contaminated with multiple seed borne diseases. Farmers should be sensitized on appropriate seed potato handling practices and there should be increased supply of certified seed potato.

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Biological, Serological and Molecular Diagnosis of Three Major Potato Viruses in Egypt

Cited by 21 publications

References 21 publications

Antiviral Activity of Lactoferrin against Potato virus x In vitro and In vivo

Antiviral Activity of Lactoferrin against Potato virus x In vitro and In vivo

Development of a Rapid Detection Method for Potato virus X by Reverse Transcription Loop-Mediated Isothermal Amplification

Seed Potato Production Practices and Quality of Farm Saved Seed Potato in Kiambu and Nyandarua Counties in Kenya

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