Biological Significance of Fc Receptor‐Bearing Cells Among Activated T Lymphocytes

Rubin, Bent; Hertel-Wulff, B

doi:10.1111/j.1365-3083.1975.tb02650.x

Cited by 37 publications

(12 citation statements)

References 26 publications

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“…Inbred mice were maintained in our breeding colony at the Immunobiology Laboratory, Statens Seruminstitut Copenhagen, Denmark (21,22) Center, Uppsala University, Uppsala, Sweden. Strain B65a mice were produced by backcrossing (B6 X CBA)F1 mice to B6 mice.…”

Section: Methodsmentioning

confidence: 99%

“…The medium consisted of 5% fetal calf serum (FCS) in a 1:1 mixture of Eagle's high amino acid medium (28) and R medium (ER); the culture time was 4-5 d. Cells were harvested and further purified using FIP (26). Such activated T cells were 30-95% blasts, >98% Thy-l.2, < 1% Ig +, <0.5% Fc-receptor-positive, and without antibody-dependent cell-mediated cytotoxic (ADCC) potential (22,23,26,27). They were tested for the percentage of Id + cells by the methods outlined above, and for specific cytotoxic T-cell (CTL) activity against SlCrlabeled target cells (L cells: C3H fibroblast cell line, H-2k; RBL-5: Rauscher virus-induced lymphoma in B6 mice, H-2b; L1210:DBA/2 leukemia, H-2d).…”

Section: Preparation and Test Of Lymphocytementioning

confidence: 99%

“…Purified FcR-T cells from different strains of mice were activated in MLC against H-2 k alloantigens. The choice and care used in this method of in vitro activation was based on extensive analysis of the resulting cell populations (22,23,26,27), which could be shown to >98% Thy-l.2 +, <0.5% FcR-, <1% surface Ig +, and without ADCC activity. These characteristics are of special importance in the evaluation of anti-Id-antibody-binding studies.…”

Section: Reactivity Of Anti-h-2 ~ Ig Preparations With Rabbit 5936 Amentioning

confidence: 99%

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Alloantigen-specific idiotype-bearing receptors on mouse T lymphocytes. I. Specificity characterization and genetic association with the heavy-chain IgG allotype.

Rubin¹,

Hertel-Wulff²,

Kimura³

1979

The Journal of Experimental Medicine

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The present study describes the qualitative reactions of a xenogeneic anti-idiotype (Id) antiserum produced in a mouse-gamma-globulin-tolerant rabbit (5,936) against B6 anti-CBA IgG antibodies. The results showed that such an anti-Id antiserum reacts specifically against anti-H-2k antibodies and against H-2k alloantigen-activated T cells from the following pairs of congenic mice: B10 (H-2b) and B10.D2 (H-2d); and A.BY (H-2b) and A.SW (H-2s), but not against C3H.SW (H-2b) and C3H.OH (H-2o); and BALB/b (H-2b) and BALB/c (H-2d). CB 20 (BALB/c mice with the Ig-1b allotype) anti-CBA T blasts also express idiotypic determinants that react with rabbit 5,936 antiserum. Thus, positive reactions are obtained between rabbit 5,936 anti-Id antiserum and anti-H-2k IgG preparations and T blasts from mice carrying the Ig-1b or Ig-1e allotype, but not from mice carrying the Ig-1a allotype. These reactions are qualitatively independent of the H-2 genotype of the Id-producing mice. Such a finding strongly suggests that the Id-bearing receptor molecules on mouse T cells are coded for by genes that are associated with the Ig heavy-chain-linkage group and not to the mouse histocompatibility complex. Furthermore, the anti-Id antibodies studied react preferentially against anti-H-2k antibodies or T cells with specificity toward the IAk-region-associated serological specificities. Thus, genes associated with the Ig heavy-chain-linkage group seem to be structural genes for at least T-cell receptors with specificity for IA-region-coded membrane antigens.

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Section: Methodsmentioning

confidence: 99%

Section: Preparation and Test Of Lymphocytementioning

confidence: 99%

Section: Reactivity Of Anti-h-2 ~ Ig Preparations With Rabbit 5936 Amentioning

confidence: 99%

See 1 more Smart Citation

Alloantigen-specific idiotype-bearing receptors on mouse T lymphocytes. I. Specificity characterization and genetic association with the heavy-chain IgG allotype.

Rubin¹,

Hertel-Wulff²,

Kimura³

1979

The Journal of Experimental Medicine

Self Cite

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“…On the basis of the results from Section 3.1. we tried to separate T from K lymphocytes (and other cells) by means of fractionation on HSA-anti-HSA columns (known t o retain adherent cells and cells with high-avidity F c receptors [9]), and on Ig-anti-Ig columns (known to retain most cells except T lymphocytes, [ l , 7-9, 23, 27, 331). Tables 5-6 and Fig.…”

Section: Effect Of Column Fractionation On In Vitro Responses Ofmentioning

confidence: 99%

Lymphocyte proliferation in vitro induced by soluble protein antigens. II. Cellular requirements

Hertel-Wulff

Rubin

1976

Eur J Immunol

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Immune guinea pig lymph node cells were fractionated on Ig anti-Ig or HSA anti-HSA affinity columns or on plastic surface in medium containing carbonyl iron. These techniques selectively removed B lymphocytes, K lymphocytes or adherent cells. The residual cells (Fc receptor-negative T lymphocytes) responded to soluble antigen in vitro in the same way or even better compared with nonfractionated cells. In addition, there was no indication that antigen-antibody complexes were superior to antigen in triggering lymph node cells or purified lymph code T lymphocytes into DNA synthesis. The results obtained suggested that memory T lymphocytes can be stimulated by antigen autonomously.

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“…suppressive B cell factor (SBF) [17]. This is also the case in T cells; T cells bearing FcR (FcRy+ T) are known to sup press the proliferation of B cells activated by pokeweed mitogen in human [18], while FcRy" T cells act as either antigen-specific or nonspecific helpers [16,20,22,27]. Kil ler cell induction in mixed lymphocyte reac tion is also suppressed by the supplement of FcRy+ T cells in mixed lymphocyte reaction culture [14], Thus, one of the roles of FcRy in immune responses seems to be that of a receptor for regulatory signals from immune complexes, therefore, FcRy+ B cells per haps play a role of immune regulators.…”

Section: Introductionmentioning

confidence: 99%

Immunological Properties of Fc Receptor on Lymphocytes

Miyama-Inaba¹,

Masuda²,

Inada³

et al. 1981

Int Arch Allergy Immunol

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The present study was undertaken to clarify the function of FcRγ⁺ and FcRγ^–– lymphocytes in anti-DNP IgE antibody responses. The helper activity was observed predominantly in FcRγ^–– T cells, but poorly in FcRγ⁺ T cells, as in the case of IgG antibody responses, indicating that the absence of FcRγ on T cell surface is a common surface characteristic to helper T cells, and that there is no close relationship between the determination of class specificity of Ig and T cell subsets divided by the presence or absence of FcRγ. Furthermore, IgE antibody responses were more dependent upon helper T cells than IgG antibody responses. The data presented in this paper also demonstrated that the precursors of anti-DNP IgE antibody-forming cells were equally contained in both FcRγ⁺ and FcRγ^–– B cells, when they were transferred into recipients with helper T cells. This is a striking contrast to IgM/IgG antibody responses, in which only FcRγ^–– B cells could differentiate into antibody-forming cells. Moreover, the precursor activity of FcRγ⁺ and FcRγ^–– B cells for IgE antibody-forming cells was not affected by the treatment of cells with suppressive B cell factor released from FcRγ⁺ B cells after binding of antigen-antibody (IgG) complexes and known to suppress the proliferation of FcRγ^–– IgM/IgG antibody-forming cell precursors. Thus, IgE B cells seem to be more heterogeneous than IgM/IgG B cells with respect to the FcRγ marker and insensitive to the regulatory effect of FcRγ⁺ B cells. IgE B cells are regulated differently from IgG B cells in B-B cell interactions.

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Biological Significance of Fc Receptor‐Bearing Cells Among Activated T Lymphocytes

Cited by 37 publications

References 26 publications

Alloantigen-specific idiotype-bearing receptors on mouse T lymphocytes. I. Specificity characterization and genetic association with the heavy-chain IgG allotype.

Alloantigen-specific idiotype-bearing receptors on mouse T lymphocytes. I. Specificity characterization and genetic association with the heavy-chain IgG allotype.

Lymphocyte proliferation in vitro induced by soluble protein antigens. II. Cellular requirements

Immunological Properties of Fc Receptor on Lymphocytes

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