2001
DOI: 10.1046/j.1365-2141.2001.03058.x
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Biological significance of MAPK, AKT and JAK‐STAT protein activation by various erythropoietic factors in normal human early erythroid cells

Abstract: Summary. The aim of this study was to identify signal transduction pathways activated by erythropoietin (EpO) and erythropoietin co-stimulatory factors (kit ligand), insulin-like growth factor, thrombopoietin, interleukin 3 and granulocyte-macrophage colony-stimulating factor) in normal human bone marrow CD34 1 cells and d 11 erythroid burst forming unit derived glycophorin 1 cells. The activation of these signal transduction pathways was further correlated with various biological effects such as (i) cell prol… Show more

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Cited by 60 publications
(53 citation statements)
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“…Western blot analysis was performed on protein extracts from cells as described [33][34][35][36][37] after the lung cancer cells were stimulated with PMV or exosomes (30 g/mL) for 5 or 10 min at 37°C. Phosphorylation of serine/threonine kinase AKT and p44/42 mitogen-activated protein kinase (MAPK) was detected by protein immunoblotting using mouse monoclonal 44/42 phospho-specific MAPK antibody and rabbit phospho-specific polyclonal antibodies (all from New England Biolabs, Beverly, MA) for each of the remainder, with horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin (Ig)G or goat anti-rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA) as secondary antibody, as described.…”
Section: Phosphorylation Of Intracellular Pathway Proteinsmentioning
confidence: 99%
See 1 more Smart Citation
“…Western blot analysis was performed on protein extracts from cells as described [33][34][35][36][37] after the lung cancer cells were stimulated with PMV or exosomes (30 g/mL) for 5 or 10 min at 37°C. Phosphorylation of serine/threonine kinase AKT and p44/42 mitogen-activated protein kinase (MAPK) was detected by protein immunoblotting using mouse monoclonal 44/42 phospho-specific MAPK antibody and rabbit phospho-specific polyclonal antibodies (all from New England Biolabs, Beverly, MA) for each of the remainder, with horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin (Ig)G or goat anti-rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA) as secondary antibody, as described.…”
Section: Phosphorylation Of Intracellular Pathway Proteinsmentioning
confidence: 99%
“…Phosphorylation of serine/threonine kinase AKT and p44/42 mitogen-activated protein kinase (MAPK) was detected by protein immunoblotting using mouse monoclonal 44/42 phospho-specific MAPK antibody and rabbit phospho-specific polyclonal antibodies (all from New England Biolabs, Beverly, MA) for each of the remainder, with horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin (Ig)G or goat anti-rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA) as secondary antibody, as described. [33][34][35][36][37] Equal loading in the lanes was evaluated by stripping the blots and reprobing them with the appropriate monoclonal or polyclonal antibodies: p42/44 anti-MAPK antibody clone 9102 and anti-AKT antibody clone 9272 (Santa Cruz Biotechnology). The membranes were developed with an ECL reagent (Amersham Life Sciences, Little Chalfont, UK), dried and exposed to film (HyperFilm, Amersham).…”
Section: Phosphorylation Of Intracellular Pathway Proteinsmentioning
confidence: 99%
“…EPO is known to stimulate three common cell survival pathways, including PI3K/Akt, ERK1/2 MAPK, and Jak-STAT in hematopoietic (25)(26)(27) and endothelial (28) cell lines. ERK and Akt activation (via phosphorylation) were assessed in H9c2 cardiomyoblasts after EPO addition and quantified by Western blot analysis and densitometry at serial time points (1-60 minutes) to determine signaling intermediates (Figure 1, a and b).…”
Section: Epo Signaling and Proliferation In H9c2 Myoblastsmentioning
confidence: 99%
“…7 Erythropoietin (EPO) and other signaling factors have demonstrable effects on the survival, proliferation, and differentiation of erythroblasts. These effects may result due to interplay between intracellular signals 8,9 and specific transcriptional regulators. 10 EPO augments HbF after acute intravenous administration, but that effect is lost after chronic use.…”
Section: Introductionmentioning
confidence: 99%
“…SCF-and EPOmediated signals interact at various points along the Ras/Raf1/ MAPK and PI3K pathways to affect specific biologic responses. 8,9 Here we explore the hypothesis that growth-related signaling pathways may coincidentally regulate HbF synthesis during adult human erythropoiesis.…”
Section: Introductionmentioning
confidence: 99%