“…Phosphorylation of serine/threonine kinase AKT and p44/42 mitogen-activated protein kinase (MAPK) was detected by protein immunoblotting using mouse monoclonal 44/42 phospho-specific MAPK antibody and rabbit phospho-specific polyclonal antibodies (all from New England Biolabs, Beverly, MA) for each of the remainder, with horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin (Ig)G or goat anti-rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA) as secondary antibody, as described. [33][34][35][36][37] Equal loading in the lanes was evaluated by stripping the blots and reprobing them with the appropriate monoclonal or polyclonal antibodies: p42/44 anti-MAPK antibody clone 9102 and anti-AKT antibody clone 9272 (Santa Cruz Biotechnology). The membranes were developed with an ECL reagent (Amersham Life Sciences, Little Chalfont, UK), dried and exposed to film (HyperFilm, Amersham).…”