Biological surface engineering: a simple system for cell pattern formation

Zhang, Shuguang; Yan, Lin; Altman, Michael D.; Lässle, Michael; Nugent, Helen M.; Frankel, Felice; Lauffenburger, Douglas A.; Whitesides, George M.; Rich, Alexander

doi:10.1016/s0142-9612(99)00014-9

Cited by 329 publications

(236 citation statements)

References 40 publications

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“…We show that use of this biological nanofiber scaffold (24,25,36) is an effective approach to facilitate the reconstruction of a continuous tissue substrate after CNS injury.…”

Section: Self-assembling Peptide Nanofiber Scaffold (Sapns)mentioning

confidence: 97%

Nano neuro knitting: Peptide nanofiber scaffold for brain repair and axon regeneration with functional return of vision

Ellis-Behnke

Liang

You

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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Nanotechnology is often associated with materials fabrication, microelectronics, and microfluidics. Until now, the use of nanotechnology and molecular self assembly in biomedicine to repair injured brain structures has not been explored. To achieve axonal regeneration after injury in the CNS, several formidable barriers must be overcome, such as scar tissue formation after tissue injury, gaps in nervous tissue formed during phagocytosis of dying cells after injury, and the failure of many adult neurons to initiate axonal extension. Using the mammalian visual system as a model, we report that a designed self-assembling peptide nanofiber scaffold creates a permissive environment for axons not only to regenerate through the site of an acute injury but also to knit the brain tissue together. In experiments using a severed optic tract in the hamster, we show that regenerated axons reconnect to target tissues with sufficient density to promote functional return of vision, as evidenced by visually elicited orienting behavior. The peptide nanofiber scaffold not only represents a previously undiscovered nanobiomedical technology for tissue repair and restoration but also raises the possibility of effective treatment of CNS and other tissue or organ trauma.

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“…We show that use of this biological nanofiber scaffold (24,25,36) is an effective approach to facilitate the reconstruction of a continuous tissue substrate after CNS injury.…”

Section: Self-assembling Peptide Nanofiber Scaffold (Sapns)mentioning

confidence: 97%

Nano neuro knitting: Peptide nanofiber scaffold for brain repair and axon regeneration with functional return of vision

Ellis-Behnke

Liang

You

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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761

490

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“…Surfaces have also been modified with chemistries that allow the covalent attachment of proteins, circumventing possible denaturation events caused by adsorption processes [76]. Self-assembled mono-layers (SAMs), which are molecular films formed by the spontaneous organization of molecules at interfaces, have also been used extensively to modify surfaces [77].…”

Section: Chemical Surface Patterning Followed By Protein Adsorptionmentioning

confidence: 99%

“…The patterning techniques including protein film UVor laser ablation [140,141], nitrocellulose paper strip protein transfer [142], microfluidic networks [112,113,143], and microcontact printing [76] have been utilized to produce such substrates. Of interest are studies in which these techniques have been used to generate concentration gradients and protein field boundaries to discover how bound factor density and spatial distrubition affect cell behavior.…”

Section: The Effects Of Cspg Surface Concentrationmentioning

confidence: 99%

The effects of proteoglycan surface patterning on neuronal pathfinding

Hlady

Hodgkinson

2007

Materialwissenschaft Werkst

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Protein micropatterning techniques are increasingly applied in cell choice assays to investigate fundamental biological phenomena that contribute to the host response to implanted biomaterials, and to explore the effects of protein stability and biological activity on cell behavior for in vitro cell studies. In the area of neuronal regeneration the protein micropatterning and cell choice assays are used to improve our understanding of the mechanisms directing nervous system during development and regenerative failure in the central nervous system (CNS) wound healing environment. In these cell assays, protein micropatterns need to be characterized for protein stability, bioactivity, and spatial distribution and then correlated with observed mammalian cell behavior using appropriate model system for CNS development and repair. This review provides the background on protein micropatterning for cell choice assays and describes some novel patterns that were developed to interrogate neuronal adaptation to inhibitory signals encountered in CNS injuries. Rationale for studying the role of proteoglycans in CNS injuriesNeurons from the central nervous system (CNS) possess a limited capacity to regenerate beyond scar tissue formation barriers in injuries even in the presence of minimal trauma to tissue organization [1,2]. A major culprit in CNS neuronal regenerative failure are the inihibitory proteoglycans (PGs), which are upregulated at the site of CNS injuries. PGs are composed of a core protein with varying numbers of attached glycosaminoglycan (GAG) side chains [3,4]. Proteoglycans are first translated intracellularly into proteins in the endoplasmic reticulum, and then GAG chains are later attached in the Golgi apparatus before secretion into the extracellular space [5][6][7][8]. The study of PGs has particularly been pronounced in the field of neurobiology and development of the nervous system. Numerous studies have concluded that PGs act as barriers that direct the migration of neural crest cells and the outgrowth direction of pathfinding neurons during development [9][10][11][12][13][14][15][16][17]. A number of these studies have additionally shown that cell guidance by PGs is based on an inhibitory signal located within the chondroitin sulfate GAG chains [9,18,19], with the result that the artificial addition of chondroitin sulfate (CS) sugars to the developing nervous system disrupts normal pathfinding [20] as well as does the removal of chondroitin sulfate chains through enzymatic treatment [9,19]. Davies et al. found that chondroitin sulfate proteoglycans (CSPG) are a major constituent of the glial scar and that dorsal root ganglion (DRG) outgrowth termination coincided with an increase in CSPG expression density [21]. Other sources have also shown that CSPGs are upregulated after CNS injuries [22][23][24][25].Interestingly, native adult CNS neurons actually posses the intrinsic ability to regenerate when the wound healing environment is prepared by eliminating inhibitory factors [23,26,27]. In additi...

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“…It has prompted numerous studies of peptides such as lego-type peptides, peptide surfactants and peptide ink. [1][2][3] 'Peptide Lego' forms well-ordered nanofiber scaffolds for 3D cell culture and for regenerative medicine. 'Peptide surfactants' are used for drug, protein and gene delivery as well as for solubilizing and stabilizing membrane proteins.…”

Section: Introductionmentioning

confidence: 99%

Investigation of Self-assembly Structure and Properties of a Novel Designed Lego-type Peptide with Double Amphiphilic Surfaces

Wang¹,

Zhao²

2010

Bulletin of the Korean Chemical Society

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A typically designed 'Peptide Lego' has two distinct surfaces: a hydrophilic side that contains the complete charge distribution and a hydrophobic side. In this article, we describe the fabrication of a unique lego-type peptide with the AEAEYAKAK sequence. The novel peptide with double amphiphilic surfaces is different from typical peptides due to special arrangement of the residues. The results of CD, FT-IR, AFM and DLS demonstrate that the peptide with the random coil characteristic was able to form stable nanostructures that were mediated by non-covalent interactions in an aqueous solution. The data further indicated that despite its different structure, the peptide was able to undergo self-assembly similar to a typical peptide. In addition, the use of hydrophobic pyrene as a model allowed the peptide to provide a new type of potential nanomaterial for drug delivery. These efforts collectively open up a new direction in the fabrication of nanomaterials that are more perfect and versatile.

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Biological surface engineering: a simple system for cell pattern formation

Cited by 329 publications

References 40 publications

Nano neuro knitting: Peptide nanofiber scaffold for brain repair and axon regeneration with functional return of vision

Nano neuro knitting: Peptide nanofiber scaffold for brain repair and axon regeneration with functional return of vision

The effects of proteoglycan surface patterning on neuronal pathfinding

Investigation of Self-assembly Structure and Properties of a Novel Designed Lego-type Peptide with Double Amphiphilic Surfaces

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