Biological toxicity of cellulose nanocrystals (CNCs) against the luxCDABE-based bioluminescent bioreporter Escherichia coli 652T7

Du, Lei; Arnholt, Kelly; Ripp, Steven; Sayler, Gary S.; Wang, Siqun; Liang, Cui; Wang, Jingkuan; Zhuang, Jie

doi:10.1007/s10646-015-1555-0

Cited by 28 publications

(26 citation statements)

References 17 publications

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“…Hosseinidoust et al 33 conrmed the biocompatibility of CNC by not detecting any damage or change in density of phagocytic and nonphagocytic cell lines during their interaction with CNC dispersions. Except for some studies 34,35 that showed some minor detrimental effects for highly elevated CNC concentrations (>350 mg l À1 ), CNC still remains a widely accepted versatile biomaterial for producing nature-friendly materials.…”

Section: Introductionmentioning

confidence: 99%

Safer-by-design hybrid nanostructures: an alternative to conventional titanium dioxide UV filters in skin care products

Shandilya

Capron

2017

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

Safer-by-design hybrid nanostructures: an alternative to conventional titanium dioxide UV filters in skin care products

Shandilya

Capron

2017

RSC Adv.

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“…Lower bioavailability of the Hg(II) ions would be connected not only with the lower bioluminescence induction, but possibly also with lower toxic effect of the mercury on the E. coli ARL1. The decrease of Hg 2+ -induced bioluminescent response of strain ARL1 in the presence of humic acids was ascribed to reduction of bioavailability of Hg 2+ via complexation with HA also by Du et al [46]. The comparison of the growth rates (Table 3) helped us to elucidate the humic acid action.…”

Section: Mercury Arl1 Bioassay and Its Interferencesmentioning

confidence: 59%

“…This assumption is supported by the lux-mer construction which uses the detoxification-elimination mechanism. Low toxicity of complexes with humic acids to E. coli was already shown by unchanged light emission of constitutive bioreporter E. coli 652T7 [46]. As a result, the positive effect of humic acid on the bacterial growth cannot be explained by protecting the cells from the toxicity of mercury by its binding.…”

Section: Mercury Arl1 Bioassay and Its Interferencesmentioning

confidence: 99%

Estimation of Hg(II) in Soil Samples by Bioluminescent Bacterial Bioreporter E. coli ARL1, and the Effect of Humic Acids and Metal Ions on the Biosensor Performance

Brányiková

Lucáková

Kuncová

et al. 2020

Sensors

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Mercury is a ubiquitous environmental pollutant of dominantly anthropogenic origin. A critical concern for human health is the introduction of mercury to the food chain; therefore, monitoring of mercury levels in agricultural soil is essential. Unfortunately, the total mercury content is not sufficiently informative as mercury can be present in different forms with variable bioavailability. Since 1990, the use of bioreporters has been investigated for assessment of the bioavailability of pollutants; however, real contaminated soils have rarely been used in these studies. In this work, a bioassay with whole-cell bacterial bioreporter Escherichia coli ARL1 was used for estimation of bioavailable concentration of mercury in 11 soil samples. The bioreporter emits bioluminescence in the presence of Hg(II). Four different pretreatments of soil samples prior to the bioassay were tested. Among them, laccase mediated extraction was found to be the most suitable over water extraction, alkaline extraction, and direct use of water-soil suspensions. Nevertheless, effect of the matrix on bioreporter signal was found to be severe and not possible to be completely eliminated by the method of standard addition. In order to elucidate the matrix role, influences of humic acid and selected metal ions present in soil on the bioreporter signal were tested separately in laboratory solutions. Humic acids were found to have a positive effect on the bioreporter growth, but a negative effect on the measured bioluminescence, likely due to shading and Hg binding resulting in decreased bioavailability. Each of the tested metal ions solutions affected the bioluminescence signal differently; cobalt (II) positively, iron (III) negatively, and the effects of iron (II) and nickel (II) were dependent on their concentrations. In conclusion, the information on bioavailable mercury estimated by bioreporter E. coli ARL1 is valuable, but the results must be interpreted with caution. The route to functional bioavailability bioassay remains long.

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“…In general, environmental conditions have a large potential for mediating the toxicity of engineered nanoparticles. Du et al (2015) evaluated the toxicity of cellulose nanocrystals (CNCs) by assessment of the response of constitutively bioluminescent luxCDABEbased bioreporter Escherichia coli 652T7. Results demonstrated that CNCs were not significantly toxic at concentrations at or below 250 mg/L, but the toxicity increased linearly as CNC concentrations increased from 300 to 2000 mg/L.…”

Section: Toxic Effect Of Engineered Nanoparticlesmentioning

confidence: 99%

Fate and toxic effects of environmental stressors: environmental control

Zhuang

Henry

et al. 2015

Ecotoxicology

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The potential for toxicants to harm organisms in the environment is influenced by the physicochemistry of the substances and their environmental behaviors and transformation within ecosystems. This special issue is composed of 20 papers that report on studies which have investigated the fate and toxicity of various toxicants including engineered nanoparticles, pharmaceuticals and personal care products, antibiotics, pathogens, heavy metals, and agricultural nutrients. The environmental transformations of these substances and how these processes affect their toxicity are emphasized. This paper highlights the important findings and perspectives of the selected papers in this special edition, with an aim of providing insights into full-scale evaluation on the toxicity of various contaminants that exist in ecosystems. General suggestions are provided for the future directions of toxicological research.

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Biological toxicity of cellulose nanocrystals (CNCs) against the luxCDABE-based bioluminescent bioreporter Escherichia coli 652T7

Cited by 28 publications

References 17 publications

Safer-by-design hybrid nanostructures: an alternative to conventional titanium dioxide UV filters in skin care products

Safer-by-design hybrid nanostructures: an alternative to conventional titanium dioxide UV filters in skin care products

Estimation of Hg(II) in Soil Samples by Bioluminescent Bacterial Bioreporter E. coli ARL1, and the Effect of Humic Acids and Metal Ions on the Biosensor Performance

Fate and toxic effects of environmental stressors: environmental control

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