Biologically active interleukin 1 in human eccrine sweat: Site-dependent variations in α/β ratios and stress-induced increased excretion

Interleukin 1 receptor antagonist in human epidermis and cultured keratinocytes

“…of'IL-1 mRNA As previously described [5,8] a high level of IL-1 a and low amount of IL-l/? were observed in extracts of normal human epidermis and in sweat (Table I).…”

“…The epidermis was separated from the dermis by heating whole skin for I min at 56°C in 0.9% sodium chloride as described [6]. Human sweat was collected from patients with spontaneous hand hyperhidrosis as described [5]. Human keratinocytes from foreskin, obtained as described by Rheinwald and Green [13], were cultured in a mixture of Dulbecco-Vogt and Ham's F12 (3:1) medium supplemented with 10% fetal calf serum (FCS) (Seromed, Berlin, Germany), insulin (0.4 &ml), adenine (24 ,@ml), transferrin (6 pg/ml), triiodothyronine (2 ng/ml), and epidermal growth factor (10 ng/ml).…”

“…Initially described as a monocyte product, IL-l is synthetized by a wide variety of cells including keratinocytes [2,3]. High levels of biologically active IL-l were found in normal unstimulated human epidermis and human sweat [4,5]. The functional and physiological relevance of this IL-1 pool is still unclear.…”

The cutaneous non-neuronal cholinergic system and smoking related dermatoses: Studies of the psoriasis variant palmoplantar pustulosis

“…In a study of the antigenic profile of the acrosyringium, constitutive expression of HLA-DR, DP, and DQ was observed on luminal acrosyringeal cells (McGregor et al, 1991). It has also been shown by in situ hybridisation that mRNAs encoding the cytokines IL-1 alpha, IL-1 beta, and tumour necrosis factor-alpha are present in normal human eccrine sweat gland duct and secretory coil epithelium but not in epidermis (Boehm et al, 1994;Didierjean et al, 1990). Moreover, intense intracellular IL-8 immunoreactivity has been reported to occur in all portions of the dermal and epidermal parts of the eccrine sweat apparatus both in normal palmar skin and in involved PPP skin (Anttila et al, 1992).…”

Sweat-Related Dermatoses: Old Concept and New Scenario

“…It also participates in metabolic, hormonal and immunological functions [1|. Human sweat contains many enzymes [2,3], a renin-like substance at a concen tration 30 times that found in the serum [4], and secretory IgA [5] and high amounts of immunoreactive mature interleukin-1 a and (3, up to 100 ug/day, are also detected in the sweat [6],In terms of pathologic states of eccrine sweat glands, textbooks and reviews traditionally devote their discus sions to 'disorders of sweating' -hyperhidrosis and anhi drosis, neoplasms of sweat glands and some structural or morphologic alterations of the gland. The discussions may briefly mention some dermatoses causing anhidrosis, e.g.…”